IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae.

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IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae. / Corbett, Mark; Xiong, Yulan; Boyne, James R. et al.
In: European Journal of Cell Biology, Vol. 85, No. 11, 03.11.2006, p. 1201-1285.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Corbett, M, Xiong, Y, Boyne, JR, Wright, DJ, Munro, E & Price, C 2006, 'IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae.', European Journal of Cell Biology, vol. 85, no. 11, pp. 1201-1285. https://doi.org/10.1016/j.ejcb.2006.08.001

APA

Corbett, M., Xiong, Y., Boyne, J. R., Wright, D. J., Munro, E., & Price, C. (2006). IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae. European Journal of Cell Biology, 85(11), 1201-1285. https://doi.org/10.1016/j.ejcb.2006.08.001

Vancouver

Corbett M, Xiong Y, Boyne JR, Wright DJ, Munro E, Price C. IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae. European Journal of Cell Biology. 2006 Nov 3;85(11):1201-1285. doi: 10.1016/j.ejcb.2006.08.001

Author

Corbett, Mark ; Xiong, Yulan ; Boyne, James R. et al. / IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae. In: European Journal of Cell Biology. 2006 ; Vol. 85, No. 11. pp. 1201-1285.

Bibtex

@article{c6b2e574bb4d43dca1754d6557d2769a,

title = "IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae.",

abstract = "In budding yeast the final stages of the cell division cycle, cytokinesis and cell separation, are distinct events that require to be coupled, both together and with mitotic exit. Here we demonstrate that mutations in genes of the mitotic exit network (MEN) prevent cell separation and are synthetically lethal in combination with both cytokinesis and septation defective mutations. Analysis of the synthetic lethal phenotypes reveals that Iqg1p functions in combination with the MEN components, Tem1p, Cdc15p Dbf20p and Dbf2p to govern the re-polarization of the actin cytoskeleton to either side of the bud neck. In addition phosphorylation of the conserved PCH protein, Hof1p, is dependent upon these activities and requires actin ring assembly. Recruitment of Dbf2p to the bud neck is dependent upon actin ring assembly and correlates with Hof1p phosphorylation. Failure to phosphorylate Hof1p results in the increased stability of the protein and its persistence at the bud neck. These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly.",

keywords = "Yeast, Cytokinesis, IQGAP, MEN, Actin",

author = "Mark Corbett and Yulan Xiong and Boyne, {James R.} and Wright, {Daniel J.} and Ewen Munro and Clive Price",

year = "2006",

month = nov,

day = "3",

doi = "10.1016/j.ejcb.2006.08.001",

language = "English",

volume = "85",

pages = "1201--1285",

journal = "European Journal of Cell Biology",

issn = "0171-9335",

publisher = "Urban und Fischer Verlag GmbH und Co. KG",

number = "11",

}

RIS

TY - JOUR

T1 - IQGAP and mitotic exit network proteins are required for cytokinesis and re-polarization of the actin cytoskeleton in the budding yeast, Saccharomyces cerevisiae.

AU - Corbett, Mark

AU - Xiong, Yulan

AU - Boyne, James R.

AU - Wright, Daniel J.

AU - Munro, Ewen

AU - Price, Clive

PY - 2006/11/3

Y1 - 2006/11/3

N2 - In budding yeast the final stages of the cell division cycle, cytokinesis and cell separation, are distinct events that require to be coupled, both together and with mitotic exit. Here we demonstrate that mutations in genes of the mitotic exit network (MEN) prevent cell separation and are synthetically lethal in combination with both cytokinesis and septation defective mutations. Analysis of the synthetic lethal phenotypes reveals that Iqg1p functions in combination with the MEN components, Tem1p, Cdc15p Dbf20p and Dbf2p to govern the re-polarization of the actin cytoskeleton to either side of the bud neck. In addition phosphorylation of the conserved PCH protein, Hof1p, is dependent upon these activities and requires actin ring assembly. Recruitment of Dbf2p to the bud neck is dependent upon actin ring assembly and correlates with Hof1p phosphorylation. Failure to phosphorylate Hof1p results in the increased stability of the protein and its persistence at the bud neck. These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly.

AB - In budding yeast the final stages of the cell division cycle, cytokinesis and cell separation, are distinct events that require to be coupled, both together and with mitotic exit. Here we demonstrate that mutations in genes of the mitotic exit network (MEN) prevent cell separation and are synthetically lethal in combination with both cytokinesis and septation defective mutations. Analysis of the synthetic lethal phenotypes reveals that Iqg1p functions in combination with the MEN components, Tem1p, Cdc15p Dbf20p and Dbf2p to govern the re-polarization of the actin cytoskeleton to either side of the bud neck. In addition phosphorylation of the conserved PCH protein, Hof1p, is dependent upon these activities and requires actin ring assembly. Recruitment of Dbf2p to the bud neck is dependent upon actin ring assembly and correlates with Hof1p phosphorylation. Failure to phosphorylate Hof1p results in the increased stability of the protein and its persistence at the bud neck. These data establish a mechanistic dependency of cell separation upon an intermediate step requiring actomyosin ring assembly.

KW - Yeast

KW - Cytokinesis

KW - IQGAP

KW - MEN

KW - Actin

U2 - 10.1016/j.ejcb.2006.08.001

DO - 10.1016/j.ejcb.2006.08.001

M3 - Journal article

VL - 85

SP - 1201

EP - 1285

JO - European Journal of Cell Biology

JF - European Journal of Cell Biology

SN - 0171-9335

IS - 11

ER -

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