Research output: Contribution to Journal/Magazine › Journal article › peer-review
| <mark>Journal publication date</mark> | 1/06/1999 |
|---|---|
| <mark>Journal</mark> | Biochemical Journal |
| Issue number | 2 |
| Volume | 340 |
| Number of pages | 7 |
| Pages (from-to) | 385-391 |
| Publication Status | Published |
| <mark>Original language</mark> | English |
A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants, This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N- glycosylated, The corresponding gene is unique in the carrot genome and induced by cold, The antifreeze protein appears to be localized within the apoplast.