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Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis. / DOUGHERTY, M. J.; HAMILTON, J. G.C.; Ward, R. D.
In: Medical and Veterinary Entomology, Vol. 8, No. 2, 04.1994, p. 119-124.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

DOUGHERTY, MJ, HAMILTON, JGC & Ward, RD 1994, 'Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis', Medical and Veterinary Entomology, vol. 8, no. 2, pp. 119-124. https://doi.org/10.1111/j.1365-2915.1994.tb00150.x

APA

DOUGHERTY, M. J., HAMILTON, J. G. C., & Ward, R. D. (1994). Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis. Medical and Veterinary Entomology, 8(2), 119-124. https://doi.org/10.1111/j.1365-2915.1994.tb00150.x

Vancouver

DOUGHERTY MJ, HAMILTON JGC, Ward RD. Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis. Medical and Veterinary Entomology. 1994 Apr;8(2):119-124. doi: 10.1111/j.1365-2915.1994.tb00150.x

Author

DOUGHERTY, M. J. ; HAMILTON, J. G.C. ; Ward, R. D. / Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis. In: Medical and Veterinary Entomology. 1994 ; Vol. 8, No. 2. pp. 119-124.

Bibtex

@article{98d10572a90244c4aa841bfbeb53ba46,
title = "Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis",
abstract = "Abstract. Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 ug of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight.",
keywords = "Diptera, Lutzomyia longipalpis, oviposition attractant, oviposition pheromone, Phlebotominae, sandfly eggs, stimulant.",
author = "DOUGHERTY, {M. J.} and HAMILTON, {J. G.C.} and Ward, {R. D.}",
year = "1994",
month = apr,
doi = "10.1111/j.1365-2915.1994.tb00150.x",
language = "English",
volume = "8",
pages = "119--124",
journal = "Medical and Veterinary Entomology",
issn = "0269-283X",
publisher = "Blackwell Publishing Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Isolation of oviposition pheromone from the eggs of the sandfly Lutzomyia longipalpis

AU - DOUGHERTY, M. J.

AU - HAMILTON, J. G.C.

AU - Ward, R. D.

PY - 1994/4

Y1 - 1994/4

N2 - Abstract. Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 ug of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight.

AB - Abstract. Semiochemical components of eggs of the sandfly Lutzomyia longipalpis (Diptera: Psychodidae) were separated by high performance liquid chromatography. HPLC fractions were examined quantitatively and qualitatively by gas chromatography (GC). A bioassay was used to determine the oviposition attraction of gravid L. longipalpis to each of the fractions separately and a peak responsible for the semiochemical activity was identified. Gravid flies were placed in individual oviposition tubes to determine if the peak of interest was an oviposition stimulant. The active semiochemical fraction attracted gravid flies for oviposition. Furthermore, egg laying was enhanced: gravid flies exposed to the pheromone oviposited earlier and laid more eggs than control flies. GC analysis indicated that 1200 eggs (2 days old) gave a yield of 12.75 ug of active pheromone. This fraction had similar HPLC and GC retention times to caryophyllene oxide, suggesting comparable polarity and molecular weight.

KW - Diptera

KW - Lutzomyia longipalpis

KW - oviposition attractant

KW - oviposition pheromone

KW - Phlebotominae

KW - sandfly eggs

KW - stimulant.

U2 - 10.1111/j.1365-2915.1994.tb00150.x

DO - 10.1111/j.1365-2915.1994.tb00150.x

M3 - Journal article

C2 - 8025318

AN - SCOPUS:0028407363

VL - 8

SP - 119

EP - 124

JO - Medical and Veterinary Entomology

JF - Medical and Veterinary Entomology

SN - 0269-283X

IS - 2

ER -