Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Isoprene synthesis protects transgenic tobacco plants from oxidative stress
AU - Vickers, Claudia E.
AU - Possell, Malcolm
AU - Cojocariu, Christian
AU - Velikova, Violeta B.
AU - Laothawornkitkul, Jullada
AU - Ryan, Annette
AU - Mullineaux, Philip M.
AU - Hewitt, C. N.
PY - 2009
Y1 - 2009
N2 - Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.
AB - Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.
KW - isoprene synthase
KW - oxidative damage
KW - ozone
KW - reactive oxygen species
KW - thermotolerance
KW - REACTION-MASS-SPECTROMETRY
KW - VOLATILE ORGANIC-COMPOUNDS
KW - PTR-MS
KW - INCREASES THERMOTOLERANCE
KW - NEGATIVE CONSEQUENCES
KW - LIPID-PEROXIDATION
KW - COMPOUND EMISSIONS
KW - HYDROGEN-PEROXIDE
KW - LEAVES
KW - OZONE
U2 - 10.1111/j.1365-3040.2009.01946.x
DO - 10.1111/j.1365-3040.2009.01946.x
M3 - Journal article
VL - 32
SP - 520
EP - 531
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 5
ER -