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Keratan sulphate in the trabecular meshwork and cornea

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Keratan sulphate in the trabecular meshwork and cornea. / Davis, Y ; Fullwood, N J ; Marcyniuk, B et al.
In: Current Eye Research, Vol. 16, No. 7, 07.1997, p. 677-686.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Davis, Y, Fullwood, NJ, Marcyniuk, B, Bonshek, R, Tullo, A & Nieduszynski, IA 1997, 'Keratan sulphate in the trabecular meshwork and cornea', Current Eye Research, vol. 16, no. 7, pp. 677-686. https://doi.org/10.1076/ceyr.16.7.677.5053

APA

Davis, Y., Fullwood, N. J., Marcyniuk, B., Bonshek, R., Tullo, A., & Nieduszynski, I. A. (1997). Keratan sulphate in the trabecular meshwork and cornea. Current Eye Research, 16(7), 677-686. https://doi.org/10.1076/ceyr.16.7.677.5053

Vancouver

Davis Y, Fullwood NJ, Marcyniuk B, Bonshek R, Tullo A, Nieduszynski IA. Keratan sulphate in the trabecular meshwork and cornea. Current Eye Research. 1997 Jul;16(7):677-686. doi: 10.1076/ceyr.16.7.677.5053

Author

Davis, Y ; Fullwood, N J ; Marcyniuk, B et al. / Keratan sulphate in the trabecular meshwork and cornea. In: Current Eye Research. 1997 ; Vol. 16, No. 7. pp. 677-686.

Bibtex

@article{c85b5fe6f3dc409d81420d454813c1b3,
title = "Keratan sulphate in the trabecular meshwork and cornea",
abstract = "Purpose. A study was made of the distribution of keratan sulphate in the human anterior chamber.Methods. The monoclonal antibody, 5-D-4, was used in immuno-electron microscopy to visualise keratan sulphate distribution in the anterior chamber of 16 normal eyes, 7 Fuchs' dystrophy corneas, and a macular dystrophy cornea.Results. Keratan sulphate was detected in normal human aqueous humour and also on the surface of trabecular cells in the uveal meshwork. Normal corneal stroma showed an increase in keratan sulphate labelling from anterior to posterior, with marked labelling in the posterior region of Descemet's membrane. The apical surface of the corneal endothelium labelled positively, but showed considerable variation in the level of labelling from cell to cell. The macular dystrophy cornea had the classic histopathological features of a type I case, including a highly abnormal Descemet's membrane. No keratan sulphate was detected in the macular dystrophy patient's corneal stroma or serum. The Fuchs' endothelial dystrophy corneas showed a normal distribution of keratan sulphate labelling in the stroma. The Fuchs' endothelial cells labelled for keratan sulphate but were highly abnormal in appearance, often exhibiting long filopodia and appearing to be actively migrating.Conclusions. This work has shown that keratan sulphate has a much wider distribution than was previously believed. The detection of keratan sulphate on the trabecular and endothelial cell surfaces also suggests a possible role for this molecule in cell adhesion and/or migration.",
keywords = "cornea, glycosaminoglycan, electron microscopy, keratan sulphate, trabecular meshwork, human, SULFATE PROTEOGLYCAN, DYSTROPHY, COLLAGEN, GLYCOSAMINOGLYCANS, OLIGOSACCHARIDES, TISSUE",
author = "Y Davis and Fullwood, {N J} and B Marcyniuk and R Bonshek and A Tullo and Nieduszynski, {I A}",
year = "1997",
month = jul,
doi = "10.1076/ceyr.16.7.677.5053",
language = "English",
volume = "16",
pages = "677--686",
journal = "Current Eye Research",
issn = "1460-2202",
publisher = "Informa Healthcare",
number = "7",

}

RIS

TY - JOUR

T1 - Keratan sulphate in the trabecular meshwork and cornea

AU - Davis, Y

AU - Fullwood, N J

AU - Marcyniuk, B

AU - Bonshek, R

AU - Tullo, A

AU - Nieduszynski, I A

PY - 1997/7

Y1 - 1997/7

N2 - Purpose. A study was made of the distribution of keratan sulphate in the human anterior chamber.Methods. The monoclonal antibody, 5-D-4, was used in immuno-electron microscopy to visualise keratan sulphate distribution in the anterior chamber of 16 normal eyes, 7 Fuchs' dystrophy corneas, and a macular dystrophy cornea.Results. Keratan sulphate was detected in normal human aqueous humour and also on the surface of trabecular cells in the uveal meshwork. Normal corneal stroma showed an increase in keratan sulphate labelling from anterior to posterior, with marked labelling in the posterior region of Descemet's membrane. The apical surface of the corneal endothelium labelled positively, but showed considerable variation in the level of labelling from cell to cell. The macular dystrophy cornea had the classic histopathological features of a type I case, including a highly abnormal Descemet's membrane. No keratan sulphate was detected in the macular dystrophy patient's corneal stroma or serum. The Fuchs' endothelial dystrophy corneas showed a normal distribution of keratan sulphate labelling in the stroma. The Fuchs' endothelial cells labelled for keratan sulphate but were highly abnormal in appearance, often exhibiting long filopodia and appearing to be actively migrating.Conclusions. This work has shown that keratan sulphate has a much wider distribution than was previously believed. The detection of keratan sulphate on the trabecular and endothelial cell surfaces also suggests a possible role for this molecule in cell adhesion and/or migration.

AB - Purpose. A study was made of the distribution of keratan sulphate in the human anterior chamber.Methods. The monoclonal antibody, 5-D-4, was used in immuno-electron microscopy to visualise keratan sulphate distribution in the anterior chamber of 16 normal eyes, 7 Fuchs' dystrophy corneas, and a macular dystrophy cornea.Results. Keratan sulphate was detected in normal human aqueous humour and also on the surface of trabecular cells in the uveal meshwork. Normal corneal stroma showed an increase in keratan sulphate labelling from anterior to posterior, with marked labelling in the posterior region of Descemet's membrane. The apical surface of the corneal endothelium labelled positively, but showed considerable variation in the level of labelling from cell to cell. The macular dystrophy cornea had the classic histopathological features of a type I case, including a highly abnormal Descemet's membrane. No keratan sulphate was detected in the macular dystrophy patient's corneal stroma or serum. The Fuchs' endothelial dystrophy corneas showed a normal distribution of keratan sulphate labelling in the stroma. The Fuchs' endothelial cells labelled for keratan sulphate but were highly abnormal in appearance, often exhibiting long filopodia and appearing to be actively migrating.Conclusions. This work has shown that keratan sulphate has a much wider distribution than was previously believed. The detection of keratan sulphate on the trabecular and endothelial cell surfaces also suggests a possible role for this molecule in cell adhesion and/or migration.

KW - cornea

KW - glycosaminoglycan

KW - electron microscopy

KW - keratan sulphate

KW - trabecular meshwork

KW - human

KW - SULFATE PROTEOGLYCAN

KW - DYSTROPHY

KW - COLLAGEN

KW - GLYCOSAMINOGLYCANS

KW - OLIGOSACCHARIDES

KW - TISSUE

U2 - 10.1076/ceyr.16.7.677.5053

DO - 10.1076/ceyr.16.7.677.5053

M3 - Journal article

VL - 16

SP - 677

EP - 686

JO - Current Eye Research

JF - Current Eye Research

SN - 1460-2202

IS - 7

ER -