Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Keratan sulphate in the trabecular meshwork and cornea
AU - Davis, Y
AU - Fullwood, N J
AU - Marcyniuk, B
AU - Bonshek, R
AU - Tullo, A
AU - Nieduszynski, I A
PY - 1997/7
Y1 - 1997/7
N2 - Purpose. A study was made of the distribution of keratan sulphate in the human anterior chamber.Methods. The monoclonal antibody, 5-D-4, was used in immuno-electron microscopy to visualise keratan sulphate distribution in the anterior chamber of 16 normal eyes, 7 Fuchs' dystrophy corneas, and a macular dystrophy cornea.Results. Keratan sulphate was detected in normal human aqueous humour and also on the surface of trabecular cells in the uveal meshwork. Normal corneal stroma showed an increase in keratan sulphate labelling from anterior to posterior, with marked labelling in the posterior region of Descemet's membrane. The apical surface of the corneal endothelium labelled positively, but showed considerable variation in the level of labelling from cell to cell. The macular dystrophy cornea had the classic histopathological features of a type I case, including a highly abnormal Descemet's membrane. No keratan sulphate was detected in the macular dystrophy patient's corneal stroma or serum. The Fuchs' endothelial dystrophy corneas showed a normal distribution of keratan sulphate labelling in the stroma. The Fuchs' endothelial cells labelled for keratan sulphate but were highly abnormal in appearance, often exhibiting long filopodia and appearing to be actively migrating.Conclusions. This work has shown that keratan sulphate has a much wider distribution than was previously believed. The detection of keratan sulphate on the trabecular and endothelial cell surfaces also suggests a possible role for this molecule in cell adhesion and/or migration.
AB - Purpose. A study was made of the distribution of keratan sulphate in the human anterior chamber.Methods. The monoclonal antibody, 5-D-4, was used in immuno-electron microscopy to visualise keratan sulphate distribution in the anterior chamber of 16 normal eyes, 7 Fuchs' dystrophy corneas, and a macular dystrophy cornea.Results. Keratan sulphate was detected in normal human aqueous humour and also on the surface of trabecular cells in the uveal meshwork. Normal corneal stroma showed an increase in keratan sulphate labelling from anterior to posterior, with marked labelling in the posterior region of Descemet's membrane. The apical surface of the corneal endothelium labelled positively, but showed considerable variation in the level of labelling from cell to cell. The macular dystrophy cornea had the classic histopathological features of a type I case, including a highly abnormal Descemet's membrane. No keratan sulphate was detected in the macular dystrophy patient's corneal stroma or serum. The Fuchs' endothelial dystrophy corneas showed a normal distribution of keratan sulphate labelling in the stroma. The Fuchs' endothelial cells labelled for keratan sulphate but were highly abnormal in appearance, often exhibiting long filopodia and appearing to be actively migrating.Conclusions. This work has shown that keratan sulphate has a much wider distribution than was previously believed. The detection of keratan sulphate on the trabecular and endothelial cell surfaces also suggests a possible role for this molecule in cell adhesion and/or migration.
KW - cornea
KW - glycosaminoglycan
KW - electron microscopy
KW - keratan sulphate
KW - trabecular meshwork
KW - human
KW - SULFATE PROTEOGLYCAN
KW - DYSTROPHY
KW - COLLAGEN
KW - GLYCOSAMINOGLYCANS
KW - OLIGOSACCHARIDES
KW - TISSUE
U2 - 10.1076/ceyr.16.7.677.5053
DO - 10.1076/ceyr.16.7.677.5053
M3 - Journal article
VL - 16
SP - 677
EP - 686
JO - Current Eye Research
JF - Current Eye Research
SN - 1460-2202
IS - 7
ER -