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Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel. / Roberts, Stephen K.; Dixon, Graham K.; Dunbar, Stuart J. et al.
In: New Phytologist, Vol. 137, No. 4, 12.1997, p. 579-585.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Roberts, SK, Dixon, GK, Dunbar, SJ & Sanders, D 1997, 'Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel', New Phytologist, vol. 137, no. 4, pp. 579-585. https://doi.org/10.1046/j.1469-8137.1997.00862.x

APA

Roberts, S. K., Dixon, G. K., Dunbar, S. J., & Sanders, D. (1997). Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel. New Phytologist, 137(4), 579-585. https://doi.org/10.1046/j.1469-8137.1997.00862.x

Vancouver

Roberts SK, Dixon GK, Dunbar SJ, Sanders D. Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel. New Phytologist. 1997 Dec;137(4):579-585. doi: 10.1046/j.1469-8137.1997.00862.x

Author

Roberts, Stephen K. ; Dixon, Graham K. ; Dunbar, Stuart J. et al. / Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus : characterization of an anion-selective efflux channel. In: New Phytologist. 1997 ; Vol. 137, No. 4. pp. 579-585.

Bibtex

@article{136e29d9e8904c3ba597d08e46a97e67,
title = "Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus: characterization of an anion-selective efflux channel",
abstract = "We have used a pulsed u.v. laser to ablate the cell wall of the filamentous fungus Aspergillus niger van Tiegh., and expose the plasma membrane at defined points along the hypha. Using the patch clamp technique, giga ohm seals (up to 20 G Omega) and recordings of plasma membrane ion channels were obtained. We describe, for the first time in fungi, a 43 pS plasma membrane anion efflux channel. Its potential role in cell signalling and pH homeostasis is discussed.",
keywords = "laser ablation, anion channel, Aspergillus, MECHANOSENSITIVE ION CHANNEL, OOMYCETE SAPROLEGNIA-FERAX, HYPHAL TIP GROWTH, GUARD-CELLS, SIGNAL-TRANSDUCTION, VOLTAGE, MICROSURGERY, CALCIUM, SACCHAROMYCES, PLANTS",
author = "Roberts, {Stephen K.} and Dixon, {Graham K.} and Dunbar, {Stuart J.} and Dale Sanders",
year = "1997",
month = dec,
doi = "10.1046/j.1469-8137.1997.00862.x",
language = "English",
volume = "137",
pages = "579--585",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "4",

}

RIS

TY - JOUR

T1 - Laser ablation of the cell wall and localized patch clamping of the plasma membrane in the filamentous fungus Aspergillus

T2 - characterization of an anion-selective efflux channel

AU - Roberts, Stephen K.

AU - Dixon, Graham K.

AU - Dunbar, Stuart J.

AU - Sanders, Dale

PY - 1997/12

Y1 - 1997/12

N2 - We have used a pulsed u.v. laser to ablate the cell wall of the filamentous fungus Aspergillus niger van Tiegh., and expose the plasma membrane at defined points along the hypha. Using the patch clamp technique, giga ohm seals (up to 20 G Omega) and recordings of plasma membrane ion channels were obtained. We describe, for the first time in fungi, a 43 pS plasma membrane anion efflux channel. Its potential role in cell signalling and pH homeostasis is discussed.

AB - We have used a pulsed u.v. laser to ablate the cell wall of the filamentous fungus Aspergillus niger van Tiegh., and expose the plasma membrane at defined points along the hypha. Using the patch clamp technique, giga ohm seals (up to 20 G Omega) and recordings of plasma membrane ion channels were obtained. We describe, for the first time in fungi, a 43 pS plasma membrane anion efflux channel. Its potential role in cell signalling and pH homeostasis is discussed.

KW - laser ablation

KW - anion channel

KW - Aspergillus

KW - MECHANOSENSITIVE ION CHANNEL

KW - OOMYCETE SAPROLEGNIA-FERAX

KW - HYPHAL TIP GROWTH

KW - GUARD-CELLS

KW - SIGNAL-TRANSDUCTION

KW - VOLTAGE

KW - MICROSURGERY

KW - CALCIUM

KW - SACCHAROMYCES

KW - PLANTS

U2 - 10.1046/j.1469-8137.1997.00862.x

DO - 10.1046/j.1469-8137.1997.00862.x

M3 - Journal article

VL - 137

SP - 579

EP - 585

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 4

ER -