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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - LC-ICP-MS analysis of inositol phosphate isomers in soil offers improved sensitivity and fine-scale mapping of inositol phosphate distribution
AU - Carroll, Joseph J.
AU - Sprigg, Colleen
AU - Chilvers, Graham
AU - Delso, Ignacio
AU - Barker, Megan
AU - Cox, Filipa
AU - Johnson, David
AU - Brearley, Charles A.
N1 - Publisher Copyright: © 2024 The Authors. Methods in Ecology and Evolution published by John Wiley & Sons Ltd on behalf of British Ecological Society.
PY - 2024/3/31
Y1 - 2024/3/31
N2 - Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.
AB - Organic forms of phosphorus (P) prevail in soils and their quantification is vital to better understand global biogeochemical cycles. P speciation in soil is commonly assessed by 31P NMR spectroscopy of sodium hydroxide-EDTA (NaOH-EDTA) extracts. A liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) method that employs NaOH-EDTA is described. Comparison with 31P NMR shows that LC-ICP-MS is up to three orders of magnitude more sensitive. It allows measurement in samples as small as 1 mg. We reveal variation of inositol phosphate distribution in Swedish boreal forest soil and identify myo- and scyllo-inositol hexakisphosphates and other isomers including scyllo-inositol pentakisphosphate. Speciation of the major inositol phosphates was not altered by long-term nitrogen fertilization.
KW - inositol phosphate isomers
KW - LC-ICP-MS
KW - nitrogen fertilization
KW - phosphorus speciation
KW - phytate
KW - soil 31P NMR
U2 - 10.1111/2041-210X.14292
DO - 10.1111/2041-210X.14292
M3 - Journal article
VL - 15
SP - 530
EP - 543
JO - Methods in Ecology and Evolution
JF - Methods in Ecology and Evolution
SN - 2041-210X
IS - 3
ER -