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Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice. / Rogers, Matthew E; Hajmová, Martina; Joshi, Manju B et al.
In: Cellular Microbiology, Vol. 10, No. 6, 06.2008, p. 1363-72.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rogers, ME, Hajmová, M, Joshi, MB, Sadlova, J, Dwyer, DM, Volf, P & Bates, PA 2008, 'Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice', Cellular Microbiology, vol. 10, no. 6, pp. 1363-72. https://doi.org/10.1111/j.1462-5822.2008.01132.x

APA

Rogers, M. E., Hajmová, M., Joshi, M. B., Sadlova, J., Dwyer, D. M., Volf, P., & Bates, P. A. (2008). Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice. Cellular Microbiology, 10(6), 1363-72. https://doi.org/10.1111/j.1462-5822.2008.01132.x

Vancouver

Rogers ME, Hajmová M, Joshi MB, Sadlova J, Dwyer DM, Volf P et al. Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice. Cellular Microbiology. 2008 Jun;10(6):1363-72. doi: 10.1111/j.1462-5822.2008.01132.x

Author

Rogers, Matthew E ; Hajmová, Martina ; Joshi, Manju B et al. / Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice. In: Cellular Microbiology. 2008 ; Vol. 10, No. 6. pp. 1363-72.

Bibtex

@article{ba7fcbe2d969461a93114fb1002afc51,
title = "Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice",
abstract = "Chitinases of trypanosomatid parasites have been proposed to fulfil various roles in their blood-feeding arthropod vectors but so far none have been directly tested using a molecular approach. We characterized the ability of Leishmania mexicana episomally transfected with LmexCht1 (the L. mexicana chitinase gene) to survive and grow within the permissive sand fly vector, Lutzomyia longipalpis. Compared with control plasmid transfectants, the overexpression of chitinase was found to increase the average number of parasites per sand fly and accelerate the escape of parasites from the peritrophic matrix-enclosed blood meal as revealed by earlier arrival at the stomodeal valve. Such flies also exhibited increased damage to the structure of the stomodeal valve, which may facilitate transmission by regurgitation. When exposed individually to BALB/c mice, those flies with chitinase-overexpressing parasites spent on average 2.4-2.5 times longer in contact with their host during feeding, compared with flies with control infections. Furthermore, the lesions that resulted from these single fly bite infections were both significantly larger and with higher final parasite burdens than controls. These data show that chitinase is a multifunctional virulence factor for L. mexicana which assists its survival in Lu. longipalpis. Specifically, this enzyme enables the parasites to colonize the anterior midgut of the sand fly more quickly, modify the sand fly stomodeal valve and affect its blood feeding, all of which combine to enhance transmission.",
keywords = "Animals, Chitinase, Host-Parasite Interactions, Insect Vectors, Leishmania mexicana, Leishmaniasis, Cutaneous, Mice, Mice, Inbred BALB C, Psychodidae, Virulence, Virulence Factors",
author = "Rogers, {Matthew E} and Martina Hajmov{\'a} and Joshi, {Manju B} and Jovana Sadlova and Dwyer, {Dennis M} and Petr Volf and Bates, {Paul A}",
year = "2008",
month = jun,
doi = "10.1111/j.1462-5822.2008.01132.x",
language = "English",
volume = "10",
pages = "1363--72",
journal = "Cellular Microbiology",
issn = "1462-5822",
publisher = "Wiley-Blackwell",
number = "6",

}

RIS

TY - JOUR

T1 - Leishmania chitinase facilitates colonization of sand fly vectors and enhances transmission to mice

AU - Rogers, Matthew E

AU - Hajmová, Martina

AU - Joshi, Manju B

AU - Sadlova, Jovana

AU - Dwyer, Dennis M

AU - Volf, Petr

AU - Bates, Paul A

PY - 2008/6

Y1 - 2008/6

N2 - Chitinases of trypanosomatid parasites have been proposed to fulfil various roles in their blood-feeding arthropod vectors but so far none have been directly tested using a molecular approach. We characterized the ability of Leishmania mexicana episomally transfected with LmexCht1 (the L. mexicana chitinase gene) to survive and grow within the permissive sand fly vector, Lutzomyia longipalpis. Compared with control plasmid transfectants, the overexpression of chitinase was found to increase the average number of parasites per sand fly and accelerate the escape of parasites from the peritrophic matrix-enclosed blood meal as revealed by earlier arrival at the stomodeal valve. Such flies also exhibited increased damage to the structure of the stomodeal valve, which may facilitate transmission by regurgitation. When exposed individually to BALB/c mice, those flies with chitinase-overexpressing parasites spent on average 2.4-2.5 times longer in contact with their host during feeding, compared with flies with control infections. Furthermore, the lesions that resulted from these single fly bite infections were both significantly larger and with higher final parasite burdens than controls. These data show that chitinase is a multifunctional virulence factor for L. mexicana which assists its survival in Lu. longipalpis. Specifically, this enzyme enables the parasites to colonize the anterior midgut of the sand fly more quickly, modify the sand fly stomodeal valve and affect its blood feeding, all of which combine to enhance transmission.

AB - Chitinases of trypanosomatid parasites have been proposed to fulfil various roles in their blood-feeding arthropod vectors but so far none have been directly tested using a molecular approach. We characterized the ability of Leishmania mexicana episomally transfected with LmexCht1 (the L. mexicana chitinase gene) to survive and grow within the permissive sand fly vector, Lutzomyia longipalpis. Compared with control plasmid transfectants, the overexpression of chitinase was found to increase the average number of parasites per sand fly and accelerate the escape of parasites from the peritrophic matrix-enclosed blood meal as revealed by earlier arrival at the stomodeal valve. Such flies also exhibited increased damage to the structure of the stomodeal valve, which may facilitate transmission by regurgitation. When exposed individually to BALB/c mice, those flies with chitinase-overexpressing parasites spent on average 2.4-2.5 times longer in contact with their host during feeding, compared with flies with control infections. Furthermore, the lesions that resulted from these single fly bite infections were both significantly larger and with higher final parasite burdens than controls. These data show that chitinase is a multifunctional virulence factor for L. mexicana which assists its survival in Lu. longipalpis. Specifically, this enzyme enables the parasites to colonize the anterior midgut of the sand fly more quickly, modify the sand fly stomodeal valve and affect its blood feeding, all of which combine to enhance transmission.

KW - Animals

KW - Chitinase

KW - Host-Parasite Interactions

KW - Insect Vectors

KW - Leishmania mexicana

KW - Leishmaniasis, Cutaneous

KW - Mice

KW - Mice, Inbred BALB C

KW - Psychodidae

KW - Virulence

KW - Virulence Factors

UR - http://www.scopus.com/inward/record.url?scp=43449132687&partnerID=8YFLogxK

U2 - 10.1111/j.1462-5822.2008.01132.x

DO - 10.1111/j.1462-5822.2008.01132.x

M3 - Journal article

C2 - 18284631

VL - 10

SP - 1363

EP - 1372

JO - Cellular Microbiology

JF - Cellular Microbiology

SN - 1462-5822

IS - 6

ER -