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Leishmania donovani: immunochemical localization and secretory mechanism of soluble acid phosphatase

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Leishmania donovani: immunochemical localization and secretory mechanism of soluble acid phosphatase. / Bates, P A; Hermes, I; Dwyer, D M.
In: Experimental Parasitology, Vol. 68, No. 3, 04.1989, p. 335-346.

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Bates PA, Hermes I, Dwyer DM. Leishmania donovani: immunochemical localization and secretory mechanism of soluble acid phosphatase. Experimental Parasitology. 1989 Apr;68(3):335-346. doi: 10.1016/0014-4894(89)90115-X

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Bates, P A ; Hermes, I ; Dwyer, D M. / Leishmania donovani : immunochemical localization and secretory mechanism of soluble acid phosphatase. In: Experimental Parasitology. 1989 ; Vol. 68, No. 3. pp. 335-346.

Bibtex

@article{f766508891e84cadb3fe0f39a318ce43,
title = "Leishmania donovani: immunochemical localization and secretory mechanism of soluble acid phosphatase",
abstract = "Monoclonal antibodies specific for the soluble, secreted acid phosphatase (EC 3.1.3.2) of Leishmania donovani were used to investigate the localization of this enzyme in extracellular promastigotes and intracellular amastigotes. Indirect immunofluorescence showed a weak general staining in the promastigote cytoplasm, together with strong fluorescence in the flagellar reservoir. Immunofluorescence studies on U937 cells infected in vitro with L. donovani showed that the pathogenic amastigote stage also produced soluble acid phosphatase. Metabolic labeling experiments using promastigotes indicated that the intracellular enzyme was soluble prior to secretion and no evidence was found for the association of secretory acid phosphatase with cell membranes after protein synthesis. The rapid release of acid phosphatase from the flagellar reservoir was energy dependent and may be coupled to beating of the flagellum. The results demonstrated that acid phosphatase was secreted into the flagellar reservoir by Leishmania promastigotes using a conventional constitutive secretory mechanism, and subsequently released from the reservoir into the extracellular medium.",
keywords = "Leishmania donovani, Protozoa , parasitic , Hemoflagellate , Acid phosphatase (EC 3.1.3.2) , Flagellar reservoir , Secretion, Promastigote , Amastigote",
author = "Bates, {P A} and I Hermes and Dwyer, {D M}",
year = "1989",
month = apr,
doi = "10.1016/0014-4894(89)90115-X",
language = "English",
volume = "68",
pages = "335--346",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Leishmania donovani

T2 - immunochemical localization and secretory mechanism of soluble acid phosphatase

AU - Bates, P A

AU - Hermes, I

AU - Dwyer, D M

PY - 1989/4

Y1 - 1989/4

N2 - Monoclonal antibodies specific for the soluble, secreted acid phosphatase (EC 3.1.3.2) of Leishmania donovani were used to investigate the localization of this enzyme in extracellular promastigotes and intracellular amastigotes. Indirect immunofluorescence showed a weak general staining in the promastigote cytoplasm, together with strong fluorescence in the flagellar reservoir. Immunofluorescence studies on U937 cells infected in vitro with L. donovani showed that the pathogenic amastigote stage also produced soluble acid phosphatase. Metabolic labeling experiments using promastigotes indicated that the intracellular enzyme was soluble prior to secretion and no evidence was found for the association of secretory acid phosphatase with cell membranes after protein synthesis. The rapid release of acid phosphatase from the flagellar reservoir was energy dependent and may be coupled to beating of the flagellum. The results demonstrated that acid phosphatase was secreted into the flagellar reservoir by Leishmania promastigotes using a conventional constitutive secretory mechanism, and subsequently released from the reservoir into the extracellular medium.

AB - Monoclonal antibodies specific for the soluble, secreted acid phosphatase (EC 3.1.3.2) of Leishmania donovani were used to investigate the localization of this enzyme in extracellular promastigotes and intracellular amastigotes. Indirect immunofluorescence showed a weak general staining in the promastigote cytoplasm, together with strong fluorescence in the flagellar reservoir. Immunofluorescence studies on U937 cells infected in vitro with L. donovani showed that the pathogenic amastigote stage also produced soluble acid phosphatase. Metabolic labeling experiments using promastigotes indicated that the intracellular enzyme was soluble prior to secretion and no evidence was found for the association of secretory acid phosphatase with cell membranes after protein synthesis. The rapid release of acid phosphatase from the flagellar reservoir was energy dependent and may be coupled to beating of the flagellum. The results demonstrated that acid phosphatase was secreted into the flagellar reservoir by Leishmania promastigotes using a conventional constitutive secretory mechanism, and subsequently released from the reservoir into the extracellular medium.

KW - Leishmania donovani

KW - Protozoa

KW - parasitic

KW - Hemoflagellate

KW - Acid phosphatase (EC 3.1.3.2)

KW - Flagellar reservoir

KW - Secretion

KW - Promastigote

KW - Amastigote

U2 - 10.1016/0014-4894(89)90115-X

DO - 10.1016/0014-4894(89)90115-X

M3 - Journal article

C2 - 2649391

VL - 68

SP - 335

EP - 346

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 3

ER -