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Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs

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Leishmania species : detection and identification by nested PCR assay from skin samples of rodent reservoirs. / Akhavan, Amir Ahmad; Mirhendi, Hossein; Khamesipour, Ali; Alimohammadian, Mohammad Hossein; Rassi, Yavar; Bates, Paul; Kamhawi, Shaden; Valenzuela, Jesus G; Arandian, Mohammad Hossein; Abdoli, Hamid; Jalali-zand, Niloufar; Jafari, Reza; Shareghi, Niloufar; Ghanei, Maryam; Yaghoobi-Ershadi, Mohammad Reza.

In: Experimental Parasitology, Vol. 126, No. 4, 12.2010, p. 552-556.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Akhavan, AA, Mirhendi, H, Khamesipour, A, Alimohammadian, MH, Rassi, Y, Bates, P, Kamhawi, S, Valenzuela, JG, Arandian, MH, Abdoli, H, Jalali-zand, N, Jafari, R, Shareghi, N, Ghanei, M & Yaghoobi-Ershadi, MR 2010, 'Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs', Experimental Parasitology, vol. 126, no. 4, pp. 552-556. https://doi.org/10.1016/j.exppara.2010.06.003

APA

Akhavan, A. A., Mirhendi, H., Khamesipour, A., Alimohammadian, M. H., Rassi, Y., Bates, P., Kamhawi, S., Valenzuela, J. G., Arandian, M. H., Abdoli, H., Jalali-zand, N., Jafari, R., Shareghi, N., Ghanei, M., & Yaghoobi-Ershadi, M. R. (2010). Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs. Experimental Parasitology, 126(4), 552-556. https://doi.org/10.1016/j.exppara.2010.06.003

Vancouver

Akhavan AA, Mirhendi H, Khamesipour A, Alimohammadian MH, Rassi Y, Bates P et al. Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs. Experimental Parasitology. 2010 Dec;126(4):552-556. https://doi.org/10.1016/j.exppara.2010.06.003

Author

Akhavan, Amir Ahmad ; Mirhendi, Hossein ; Khamesipour, Ali ; Alimohammadian, Mohammad Hossein ; Rassi, Yavar ; Bates, Paul ; Kamhawi, Shaden ; Valenzuela, Jesus G ; Arandian, Mohammad Hossein ; Abdoli, Hamid ; Jalali-zand, Niloufar ; Jafari, Reza ; Shareghi, Niloufar ; Ghanei, Maryam ; Yaghoobi-Ershadi, Mohammad Reza. / Leishmania species : detection and identification by nested PCR assay from skin samples of rodent reservoirs. In: Experimental Parasitology. 2010 ; Vol. 126, No. 4. pp. 552-556.

Bibtex

@article{b15059a6cdaa43579363958441b50748,
title = "Leishmania species: detection and identification by nested PCR assay from skin samples of rodent reservoirs",
abstract = "Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L. gerbilli and L. turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L. turanica, 1.1% by L. major and L. gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations.",
keywords = "Animals, DNA Primers, DNA, Protozoan, Disease Reservoirs, Electrophoresis, Agar Gel, Gerbillinae, Iran, Leishmania, Leishmaniasis, Cutaneous, Polymerase Chain Reaction, Rodent Diseases, Skin",
author = "Akhavan, {Amir Ahmad} and Hossein Mirhendi and Ali Khamesipour and Alimohammadian, {Mohammad Hossein} and Yavar Rassi and Paul Bates and Shaden Kamhawi and Valenzuela, {Jesus G} and Arandian, {Mohammad Hossein} and Hamid Abdoli and Niloufar Jalali-zand and Reza Jafari and Niloufar Shareghi and Maryam Ghanei and Yaghoobi-Ershadi, {Mohammad Reza}",
year = "2010",
month = dec,
doi = "10.1016/j.exppara.2010.06.003",
language = "English",
volume = "126",
pages = "552--556",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Leishmania species

T2 - detection and identification by nested PCR assay from skin samples of rodent reservoirs

AU - Akhavan, Amir Ahmad

AU - Mirhendi, Hossein

AU - Khamesipour, Ali

AU - Alimohammadian, Mohammad Hossein

AU - Rassi, Yavar

AU - Bates, Paul

AU - Kamhawi, Shaden

AU - Valenzuela, Jesus G

AU - Arandian, Mohammad Hossein

AU - Abdoli, Hamid

AU - Jalali-zand, Niloufar

AU - Jafari, Reza

AU - Shareghi, Niloufar

AU - Ghanei, Maryam

AU - Yaghoobi-Ershadi, Mohammad Reza

PY - 2010/12

Y1 - 2010/12

N2 - Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L. gerbilli and L. turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L. turanica, 1.1% by L. major and L. gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations.

AB - Many rodent species act as reservoir hosts of zoonotic cutaneous leishmaniasis in endemic areas. In the present study a simple and reliable assay based on nested PCR was developed for the detection and identification of Leishmania parasites from rodent skin samples. We designed Leishmania-specific primers that successfully amplified ITS regions of Leishmania major, Leishmania gerbilli and Leishmania turanica using nested PCR. Out of 95 field collected Rhombomys opimus, 21 were positive by microscopic examination and 48 by nested PCR. The percentage of gerbils infected with L. major, L. gerbilli and L. turanica was 3.2%, 1.1% and 27.4%, respectively. In 15.8% of the rodents, we found mixed natural infections by L. major and L. turanica, 1.1% by L. major and L. gerbilli, and 2.1% by the three species. We concluded that this method is simple and reliable for detecting and identifying Leishmania species circulating in rodent populations.

KW - Animals

KW - DNA Primers

KW - DNA, Protozoan

KW - Disease Reservoirs

KW - Electrophoresis, Agar Gel

KW - Gerbillinae

KW - Iran

KW - Leishmania

KW - Leishmaniasis, Cutaneous

KW - Polymerase Chain Reaction

KW - Rodent Diseases

KW - Skin

U2 - 10.1016/j.exppara.2010.06.003

DO - 10.1016/j.exppara.2010.06.003

M3 - Journal article

C2 - 20566364

VL - 126

SP - 552

EP - 556

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 4

ER -