TY - JOUR

T1 - Leucocyte and endothelial cell adhesion molecule expression in porcine histiocytic leiomyofibrosarcoma

AU - Whyte, A

AU - Ockleford, C D

AU - Byrne, S

AU - Hubbard, A

AU - Woolley, S T

PY - 1996/11

Y1 - 1996/11

N2 - A histiocytic sarcoma was present at birth in a pig. On the basis of ultrastructure and structural-protein composition (presence of a-smooth-muscle actin but not keratin), the sarcoma component was identified as a leiomyofibrosarcoma. Lipid-laden macrophages (histiocytes), which permeated the tumour in an apparently random fashion, were somewhat atypical in that they were negative for some macrophage markers; they gave a reaction, however, for CDw14. Despite its aggressive metastatic capacity, this tumour occurred almost exclusively in the subcutis, dermis and skeletal muscle. The tumour was extensively vascularized with many small capillaries which did not express E-selectin (CD69E), MHC class II or the L-selectin (CD69L) ligand, markers characteristic of inflamed (activated) endothelial cells in pig skin. Significant numbers of the histiocytes were positive for the integrins CD18 and VLA-4 (CD49d), indicating involvement of integrin pathways in the spread or growth, or both, of the leiomyofibrosarcoma. Most of the fibrous sarcoma cells also had extensive reactivity with an antibody to the standard variant form of CD44 (CD44s). (C) 1996 W.B. Saunders Company Limited

AB - A histiocytic sarcoma was present at birth in a pig. On the basis of ultrastructure and structural-protein composition (presence of a-smooth-muscle actin but not keratin), the sarcoma component was identified as a leiomyofibrosarcoma. Lipid-laden macrophages (histiocytes), which permeated the tumour in an apparently random fashion, were somewhat atypical in that they were negative for some macrophage markers; they gave a reaction, however, for CDw14. Despite its aggressive metastatic capacity, this tumour occurred almost exclusively in the subcutis, dermis and skeletal muscle. The tumour was extensively vascularized with many small capillaries which did not express E-selectin (CD69E), MHC class II or the L-selectin (CD69L) ligand, markers characteristic of inflamed (activated) endothelial cells in pig skin. Significant numbers of the histiocytes were positive for the integrins CD18 and VLA-4 (CD49d), indicating involvement of integrin pathways in the spread or growth, or both, of the leiomyofibrosarcoma. Most of the fibrous sarcoma cells also had extensive reactivity with an antibody to the standard variant form of CD44 (CD44s). (C) 1996 W.B. Saunders Company Limited

KW - MONOCLONAL-ANTIBODIES

KW - E-SELECTIN

KW - LEIOMYOSARCOMA

KW - PROTEINS

KW - LIGANDS

KW - TISSUE

U2 - 10.1016/S0021-9975(96)80076-4

DO - 10.1016/S0021-9975(96)80076-4

M3 - Journal article

VL - 115

SP - 429

EP - 440

JO - Journal of Comparative Pathology

JF - Journal of Comparative Pathology

SN - 0021-9975

IS - 4

ER -