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Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation

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Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation. / Priestley, Megan; Hains, Anna ; Mulholland, Iashia et al.
bioRxiv, 2024.

Research output: Working paper › Preprint

Harvard

Priestley, M, Hains, A, Mulholland, I, Spijkers-Shaw, S, Zubkova, O, Dyer, D & Saunders, A 2024 'Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation' bioRxiv. https://doi.org/10.1101/2024.05.21.595098

APA

Priestley, M., Hains, A., Mulholland, I., Spijkers-Shaw, S., Zubkova, O., Dyer, D., & Saunders, A. (2024). Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation. bioRxiv. https://doi.org/10.1101/2024.05.21.595098

Vancouver

Priestley M, Hains A, Mulholland I, Spijkers-Shaw S, Zubkova O, Dyer D et al. Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation. bioRxiv. 2024 Jun 5. doi: 10.1101/2024.05.21.595098

Author

Priestley, Megan ; Hains, Anna ; Mulholland, Iashia et al. / Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation. bioRxiv, 2024.

Bibtex

@techreport{d35278641ca345f29c059b84c220d93f,
title = "Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation",
abstract = "The glycocalyx is a proteoglycan-rich layer present on the surface of all mammalian cells that is particularly prevalent on endothelial cells lining the vasculature. It has been hypothesized that the glycocalyx mediates leukocyte migration by masking adhesion molecules and reducing leukocyte adhesion to the endothelium. Leukocyte recruitment is a key driver of inflammatory diseases, including the chronic skin disease, psoriasis. Here, we show that leukocytes express heparan sulfate, an important glycocalyx component, on their cell surface which is lost in response to psoriasis-like skin inflammation, whilst endothelial heparan sulfate expression is not affected. Treatment with a heparan sulfate mimetic during psoriasis-like skin inflammation protected heparan sulfate from cleavage by heparanase and resulted in reduced leukocyte accumulation in skin, yet unexpectedly, led to increased clinical signs of inflammation due to reduced Treg numbers. These findings reshape our understanding of immune cell recruitment by revealing the presence and function of a heparan sulfate glycocalyx on immune cells and highlight the complex effects of heparanase inhibitors on the immune response in this context.",
author = "Megan Priestley and Anna Hains and Iashia Mulholland and Sam Spijkers-Shaw and Olga Zubkova and Douglas Dyer and Amy Saunders",
year = "2024",
month = jun,
day = "5",
doi = "10.1101/2024.05.21.595098",
language = "English",
publisher = "bioRxiv",
type = "WorkingPaper",
institution = "bioRxiv",

}

RIS

TY - UNPB

T1 - Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation

AU - Priestley, Megan

AU - Hains, Anna

AU - Mulholland, Iashia

AU - Spijkers-Shaw, Sam

AU - Zubkova, Olga

AU - Dyer, Douglas

AU - Saunders, Amy

PY - 2024/6/5

Y1 - 2024/6/5

N2 - The glycocalyx is a proteoglycan-rich layer present on the surface of all mammalian cells that is particularly prevalent on endothelial cells lining the vasculature. It has been hypothesized that the glycocalyx mediates leukocyte migration by masking adhesion molecules and reducing leukocyte adhesion to the endothelium. Leukocyte recruitment is a key driver of inflammatory diseases, including the chronic skin disease, psoriasis. Here, we show that leukocytes express heparan sulfate, an important glycocalyx component, on their cell surface which is lost in response to psoriasis-like skin inflammation, whilst endothelial heparan sulfate expression is not affected. Treatment with a heparan sulfate mimetic during psoriasis-like skin inflammation protected heparan sulfate from cleavage by heparanase and resulted in reduced leukocyte accumulation in skin, yet unexpectedly, led to increased clinical signs of inflammation due to reduced Treg numbers. These findings reshape our understanding of immune cell recruitment by revealing the presence and function of a heparan sulfate glycocalyx on immune cells and highlight the complex effects of heparanase inhibitors on the immune response in this context.

AB - The glycocalyx is a proteoglycan-rich layer present on the surface of all mammalian cells that is particularly prevalent on endothelial cells lining the vasculature. It has been hypothesized that the glycocalyx mediates leukocyte migration by masking adhesion molecules and reducing leukocyte adhesion to the endothelium. Leukocyte recruitment is a key driver of inflammatory diseases, including the chronic skin disease, psoriasis. Here, we show that leukocytes express heparan sulfate, an important glycocalyx component, on their cell surface which is lost in response to psoriasis-like skin inflammation, whilst endothelial heparan sulfate expression is not affected. Treatment with a heparan sulfate mimetic during psoriasis-like skin inflammation protected heparan sulfate from cleavage by heparanase and resulted in reduced leukocyte accumulation in skin, yet unexpectedly, led to increased clinical signs of inflammation due to reduced Treg numbers. These findings reshape our understanding of immune cell recruitment by revealing the presence and function of a heparan sulfate glycocalyx on immune cells and highlight the complex effects of heparanase inhibitors on the immune response in this context.

U2 - 10.1101/2024.05.21.595098

DO - 10.1101/2024.05.21.595098

M3 - Preprint

BT - Leukocytes have a heparan sulfate glycocalyx that regulates recruitment during inflammation

PB - bioRxiv

ER -