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LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

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LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. / Benedetto, Alex; Bambade, Timothee; Au, Catherine; Tullet, Jennifer; Dang, Hairuo; Cetnar, Kalina; Cabreiro, Filipe; Gems, David.

In: Biorxiv, 28.09.2017.

Research output: Contribution to journalJournal article

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APA

Benedetto, A., Bambade, T., Au, C., Tullet, J., Dang, H., Cetnar, K., Cabreiro, F., & Gems, D. (2017). LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. Biorxiv. https://www.biorxiv.org/content/early/2017/09/28/195347

Vancouver

Benedetto A, Bambade T, Au C, Tullet J, Dang H, Cetnar K et al. LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. Biorxiv. 2017 Sep 28.

Author

Benedetto, Alex ; Bambade, Timothee ; Au, Catherine ; Tullet, Jennifer ; Dang, Hairuo ; Cetnar, Kalina ; Cabreiro, Filipe ; Gems, David. / LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays. In: Biorxiv. 2017.

Bibtex

@article{574851b190a24966b5fe15958ff704e0,
title = "LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays",
abstract = "Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.",
keywords = "Nematodes, high-throughput, autophagy, oxidative stress, elegans, automated analysis, survival, heat-shock, infection, ageing",
author = "Alex Benedetto and Timothee Bambade and Catherine Au and Jennifer Tullet and Hairuo Dang and Kalina Cetnar and Filipe Cabreiro and David Gems",
year = "2017",
month = sep,
day = "28",
language = "English",
journal = "Biorxiv",
publisher = "Cold Spring Harbor Laboratory Press",

}

RIS

TY - JOUR

T1 - LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

AU - Benedetto, Alex

AU - Bambade, Timothee

AU - Au, Catherine

AU - Tullet, Jennifer

AU - Dang, Hairuo

AU - Cetnar, Kalina

AU - Cabreiro, Filipe

AU - Gems, David

PY - 2017/9/28

Y1 - 2017/9/28

N2 - Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.

AB - Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.

KW - Nematodes

KW - high-throughput

KW - autophagy

KW - oxidative stress

KW - elegans

KW - automated analysis

KW - survival

KW - heat-shock

KW - infection

KW - ageing

M3 - Journal article

JO - Biorxiv

JF - Biorxiv

ER -