Rights statement: This is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, 222, 2016 DOI: 10.1016/j.snb.2015.08.110
Accepted author manuscript, 1.96 MB, PDF document
Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Magnet bioreporter device for ecological toxicity assessment on heavy metal contamination of coal cinder sites
AU - Jia, Jianli
AU - Li, Hanbing
AU - Zong, Shuang
AU - Jiang, Bo
AU - Li, Guanghe
AU - Ejenavi, Odafe
AU - Zhu, Jingrong
AU - Zhang, Dayi
N1 - Date of Acceptance: 11/04/2015 This is the author’s version of a work that was accepted for publication in Sensors and Actuators B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators B: Chemical, 222, 2016 DOI: 10.1016/j.snb.2015.08.110
PY - 2016/1
Y1 - 2016/1
N2 - A novel magnet bioreporter device was developed in this research for soil toxicity assessment, via magnetic nanoparticles functionalized whole-cell bioreporters. The whole-cell bioreporter ADPWH-recA kept response capability to DNA damage after magnetic nanoparticles (MNPs) functionalization, and could be harvested from soil samples by permanent magnet to reduce the soil particle disturbance. Compared to conventional treatments applying bioreporter directly in soil-water mixture (SW-M treatment) or supernatant (SW-S treatment), MNPs functionalized bioreporter via the magnet device (MFB) treatment achieved high sensitivity to evaluate the toxicity and bioavailability of chromium contamination in soils from 10 mg/kg to 5000 mg/kg soil dry weight. The MNPs functionalized bioreporter also achieved high reproducibility with pH value from 5.0 to 9.0, salinity from 0% to 3% and temperature from 20 °C to 37 °C. A case study was carried out on the ecological toxicity assessment of heavy metal contamination at the coal cinder site via the magnet bioreporter device. The heavy metal toxicity declined with the increasing distance to the coal cinder point, and a significant accumulation of heavy metal toxicity was observed along the vertical distribution. No direct link was found between the pollution load index (PLI) and heavy metal toxicity, and the results suggested the bioreporter test monitored the toxicity of heavy metals in soils and was an important approach for ecological risk assessment. Magnet bioreporter device also offered the high throughput biological measurement and was feasible for in situ monitoring.
AB - A novel magnet bioreporter device was developed in this research for soil toxicity assessment, via magnetic nanoparticles functionalized whole-cell bioreporters. The whole-cell bioreporter ADPWH-recA kept response capability to DNA damage after magnetic nanoparticles (MNPs) functionalization, and could be harvested from soil samples by permanent magnet to reduce the soil particle disturbance. Compared to conventional treatments applying bioreporter directly in soil-water mixture (SW-M treatment) or supernatant (SW-S treatment), MNPs functionalized bioreporter via the magnet device (MFB) treatment achieved high sensitivity to evaluate the toxicity and bioavailability of chromium contamination in soils from 10 mg/kg to 5000 mg/kg soil dry weight. The MNPs functionalized bioreporter also achieved high reproducibility with pH value from 5.0 to 9.0, salinity from 0% to 3% and temperature from 20 °C to 37 °C. A case study was carried out on the ecological toxicity assessment of heavy metal contamination at the coal cinder site via the magnet bioreporter device. The heavy metal toxicity declined with the increasing distance to the coal cinder point, and a significant accumulation of heavy metal toxicity was observed along the vertical distribution. No direct link was found between the pollution load index (PLI) and heavy metal toxicity, and the results suggested the bioreporter test monitored the toxicity of heavy metals in soils and was an important approach for ecological risk assessment. Magnet bioreporter device also offered the high throughput biological measurement and was feasible for in situ monitoring.
KW - Heavy metal
KW - Magnet bioreporter device
KW - Magnetic nanoparticles (MNPs)
KW - Toxicity
KW - Whole-cell bioreporter
U2 - 10.1016/j.snb.2015.08.110
DO - 10.1016/j.snb.2015.08.110
M3 - Journal article
AN - SCOPUS:84940518833
VL - 222
SP - 290
EP - 299
JO - Sensors and Actuators B: Chemical
JF - Sensors and Actuators B: Chemical
SN - 0925-4005
M1 - 18965
ER -