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Male-biased mutation rate and divergence in autosomal, Z-linked and W-linked introns of chicken and turkey.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published
  • Erik Axelsson
  • Nick G. C. Smith
  • Hannah Sundström
  • Sofia Berlin
  • Hans Ellegren
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<mark>Journal publication date</mark>08/2004
<mark>Journal</mark>Molecular Biology and Evolution
Issue number8
Volume21
Number of pages10
Pages (from-to)1538-1547
Publication StatusPublished
<mark>Original language</mark>English

Abstract

To investigate mutation-rate variation between autosomes and sex chromosomes in the avian genome, we have analyzed divergence between chicken (Gallus gallus) and turkey (Meleagris galopavo) sequences from 33 autosomal, 28 Z-linked, and 14 W-linked introns with a total ungapped alignment length of approximately 43,000 bp. There are pronounced differences in the mean divergence among autosomes and sex chromosomes (autosomes [A] = 10.08%, Z chromosome = 10.99%, and W chromosome = 5.74%), and we use these data to estimate the male-to-female mutation-rate ratio (m) from Z/A, Z/W, and A/W comparisons at 1.71, 2.37, and 2.52, respectively. Because the m estimates of the three comparisons do not differ significantly, we find no statistical support for a specific reduction in the Z chromosome mutation rate (Z reduction estimated at 4.89%, P = 0.286). The idea of mutation-rate reduction in the sex chromosome hemizygous in one sex (i.e., X in mammals, Z in birds) has been suggested on the basis of theory on adaptive mutation-rate evolution. If it exists in birds, the effect would, thus, seem to be weak; a preliminary power analysis suggests that it is significantly less than 18%. Because divergence may vary within chromosomal classes as a result of variation in mutation and/or selection, we developed a novel double-bootstrapping method, bootstrapping both by introns and sites from concatenated alignments, to estimate confidence intervals for chromosomal class rates and for m. The narrowest interval for the m estimate is 1.88 to 2.97 from the Z/W comparison. We also estimated m using maximum likelihood on data from all three chromosome classes; this method yielded m = 2.47 and approximate 95% confidence intervals of 2.27 to 2.68. Our data are broadly consistent with the idea that mutation-rate differences between chromosomal classes can be explained by the male mutation bias alone.