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Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly

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Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly. / Norris, Karl; Mishukova, Oksana; Zykwinska, Agata; Colliec-Jouault, Sylvia; Sinquin, Corinne; Koptioug , Andrei; Cuenot, Stéphane; Kerns, Jemma Gillian; Surmeneva, Maria; Surmenev, Roman; Douglas, Timothy Edward Lim.

In: Micromachines, Vol. 10, No. 1, 68, 19.01.2019.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Norris, K, Mishukova, O, Zykwinska, A, Colliec-Jouault, S, Sinquin, C, Koptioug , A, Cuenot, S, Kerns, JG, Surmeneva, M, Surmenev, R & Douglas, TEL 2019, 'Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly', Micromachines, vol. 10, no. 1, 68. https://doi.org/10.3390/mi10010068

APA

Norris, K., Mishukova, O., Zykwinska, A., Colliec-Jouault, S., Sinquin, C., Koptioug , A., Cuenot, S., Kerns, J. G., Surmeneva, M., Surmenev, R., & Douglas, T. E. L. (2019). Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly. Micromachines, 10(1), [68]. https://doi.org/10.3390/mi10010068

Vancouver

Norris K, Mishukova O, Zykwinska A, Colliec-Jouault S, Sinquin C, Koptioug A et al. Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly. Micromachines. 2019 Jan 19;10(1). 68. https://doi.org/10.3390/mi10010068

Author

Norris, Karl ; Mishukova, Oksana ; Zykwinska, Agata ; Colliec-Jouault, Sylvia ; Sinquin, Corinne ; Koptioug , Andrei ; Cuenot, Stéphane ; Kerns, Jemma Gillian ; Surmeneva, Maria ; Surmenev, Roman ; Douglas, Timothy Edward Lim. / Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly. In: Micromachines. 2019 ; Vol. 10, No. 1.

Bibtex

@article{0f11b4d5dd8a4163a1ea3103b34d51e3,
title = "Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly",
abstract = "Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation.",
keywords = "marine exopolysaccharide, collagen, surface modification, Ti6Al4V",
author = "Karl Norris and Oksana Mishukova and Agata Zykwinska and Sylvia Colliec-Jouault and Corinne Sinquin and Andrei Koptioug and St{\'e}phane Cuenot and Kerns, {Jemma Gillian} and Maria Surmeneva and Roman Surmenev and Douglas, {Timothy Edward Lim}",
year = "2019",
month = jan,
day = "19",
doi = "10.3390/mi10010068",
language = "English",
volume = "10",
journal = "Micromachines",
number = "1",

}

RIS

TY - JOUR

T1 - Marine Polysaccharide-Collagen Coatings on Ti6Al4V Alloy Formed by Self-Assembly

AU - Norris, Karl

AU - Mishukova, Oksana

AU - Zykwinska, Agata

AU - Colliec-Jouault, Sylvia

AU - Sinquin, Corinne

AU - Koptioug , Andrei

AU - Cuenot, Stéphane

AU - Kerns, Jemma Gillian

AU - Surmeneva, Maria

AU - Surmenev, Roman

AU - Douglas, Timothy Edward Lim

PY - 2019/1/19

Y1 - 2019/1/19

N2 - Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation.

AB - Polysaccharides of marine origin are gaining interest as biomaterial components. Bacteria derived from deep-sea hydrothermal vents can produce sulfated exopolysaccharides (EPS), which can influence cell behavior. The use of such polysaccharides as components of organic, collagen fibril-based coatings on biomaterial surfaces remains unexplored. In this study, collagen fibril coatings enriched with HE800 and GY785 EPS derivatives were deposited on titanium alloy (Ti6Al4V) scaffolds produced by rapid prototyping and subjected to physicochemical and cell biological characterization. Coatings were formed by a self-assembly process whereby polysaccharides were added to acidic collagen molecule solution, followed by neutralization to induced self-assembly of collagen fibrils. Fibril formation resulted in collagen hydrogel formation. Hydrogels formed directly on Ti6Al4V surfaces, and fibrils adsorbed onto the surface. Scanning electron microscopy (SEM) analysis of collagen fibril coatings revealed association of polysaccharides with fibrils. Cell biological characterization revealed good cell adhesion and growth on bare Ti6Al4V surfaces, as well as coatings of collagen fibrils only and collagen fibrils enhanced with HE800 and GY785 EPS derivatives. Hence, the use of both EPS derivatives as coating components is feasible. Further work should focus on cell differentiation.

KW - marine exopolysaccharide

KW - collagen

KW - surface modification

KW - Ti6Al4V

U2 - 10.3390/mi10010068

DO - 10.3390/mi10010068

M3 - Journal article

VL - 10

JO - Micromachines

JF - Micromachines

IS - 1

M1 - 68

ER -