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Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1)

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Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1). / Taylor, Mark; Moore, Karen; Murray, Johanne et al.
In: DNA Repair, Vol. 10, No. 11, 10.11.2011, p. 1154-1163.

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Taylor M, Moore K, Murray J, Aves SJ, Price C. Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1). DNA Repair. 2011 Nov 10;10(11):1154-1163. doi: 10.1016/j.dnarep.2011.09.001

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Taylor, Mark ; Moore, Karen ; Murray, Johanne et al. / Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1). In: DNA Repair. 2011 ; Vol. 10, No. 11. pp. 1154-1163.

Bibtex

@article{fd2cfc9d0bef4f148bb067887a3932c3,
title = "Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1)",
abstract = "Initiation of DNA replication in eukaryotes is a highly conserved and ordered process involving the co-ordinated, stepwise association of distinct proteins at multiple origins of replication throughout the genome. Here, taking Schizosaccharomyces pombe as a model, the role of Rad4(TopBP1) in the assembly of the replication complex has been examined. Quantitative chromatin immunoprecipitation experiments confirm that Rad4(TopBP1) associates with origins of DNA replication and, in addition, demonstrate that the protein is not present within the active replisome. A direct interaction between Rad4(TopBP1) and Mcm10 is shown and this is reflected in the Rad4(TopBP1)-dependent origin association of Mcm10. Rad4(TopBP1) is also shown to interact with Sld2 and Sld3 and to be required for the stable origin association of these two proteins. Rad4(TopBP1) chromatin association at stalled replication forks was found to be dependent upon the checkpoint protein Rad9, which was not required for Rad4(TopBP1) origin association. Comparison of the levels of chromatin association at origins of replication and stalled replication forks and the differential requirement for Rad9 suggest functional differences for Rad4(TopBP1) at these distinct sites.",
keywords = " TopBP1, Eukaryotic DNA replication , Checkpoint control",
author = "Mark Taylor and Karen Moore and Johanne Murray and Aves, {Stephen J} and Clive Price",
note = "Copyright {\textcopyright} 2011 Elsevier B.V. All rights reserved.",
year = "2011",
month = nov,
day = "10",
doi = "10.1016/j.dnarep.2011.09.001",
language = "English",
volume = "10",
pages = "1154--1163",
journal = "DNA Repair",
publisher = "Elsevier",
number = "11",

}

RIS

TY - JOUR

T1 - Mcm10 interacts with Rad4/Cut5(TopBP1) and its association with origins of DNA replication is dependent on Rad4/Cut5(TopBP1)

AU - Taylor, Mark

AU - Moore, Karen

AU - Murray, Johanne

AU - Aves, Stephen J

AU - Price, Clive

N1 - Copyright © 2011 Elsevier B.V. All rights reserved.

PY - 2011/11/10

Y1 - 2011/11/10

N2 - Initiation of DNA replication in eukaryotes is a highly conserved and ordered process involving the co-ordinated, stepwise association of distinct proteins at multiple origins of replication throughout the genome. Here, taking Schizosaccharomyces pombe as a model, the role of Rad4(TopBP1) in the assembly of the replication complex has been examined. Quantitative chromatin immunoprecipitation experiments confirm that Rad4(TopBP1) associates with origins of DNA replication and, in addition, demonstrate that the protein is not present within the active replisome. A direct interaction between Rad4(TopBP1) and Mcm10 is shown and this is reflected in the Rad4(TopBP1)-dependent origin association of Mcm10. Rad4(TopBP1) is also shown to interact with Sld2 and Sld3 and to be required for the stable origin association of these two proteins. Rad4(TopBP1) chromatin association at stalled replication forks was found to be dependent upon the checkpoint protein Rad9, which was not required for Rad4(TopBP1) origin association. Comparison of the levels of chromatin association at origins of replication and stalled replication forks and the differential requirement for Rad9 suggest functional differences for Rad4(TopBP1) at these distinct sites.

AB - Initiation of DNA replication in eukaryotes is a highly conserved and ordered process involving the co-ordinated, stepwise association of distinct proteins at multiple origins of replication throughout the genome. Here, taking Schizosaccharomyces pombe as a model, the role of Rad4(TopBP1) in the assembly of the replication complex has been examined. Quantitative chromatin immunoprecipitation experiments confirm that Rad4(TopBP1) associates with origins of DNA replication and, in addition, demonstrate that the protein is not present within the active replisome. A direct interaction between Rad4(TopBP1) and Mcm10 is shown and this is reflected in the Rad4(TopBP1)-dependent origin association of Mcm10. Rad4(TopBP1) is also shown to interact with Sld2 and Sld3 and to be required for the stable origin association of these two proteins. Rad4(TopBP1) chromatin association at stalled replication forks was found to be dependent upon the checkpoint protein Rad9, which was not required for Rad4(TopBP1) origin association. Comparison of the levels of chromatin association at origins of replication and stalled replication forks and the differential requirement for Rad9 suggest functional differences for Rad4(TopBP1) at these distinct sites.

KW - TopBP1

KW - Eukaryotic DNA replication

KW - Checkpoint control

UR - http://www.scopus.com/inward/record.url?scp=80054757845&partnerID=8YFLogxK

U2 - 10.1016/j.dnarep.2011.09.001

DO - 10.1016/j.dnarep.2011.09.001

M3 - Journal article

C2 - 21945095

VL - 10

SP - 1154

EP - 1163

JO - DNA Repair

JF - DNA Repair

IS - 11

ER -