Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Mesenchymal stem cell (MSC) viability on PVA and PCL polymer coated hydroxyapatite scaffolds derived from cuttlefish
AU - Siddiqi, S.A.
AU - Manzoor, F.
AU - Jamal, A.
AU - Tariq, M.
AU - Ahmad, R.
AU - Kamran, M.
AU - Chaudhry, A.
AU - Rehman, I.U.
PY - 2016/4/1
Y1 - 2016/4/1
N2 - In the present study, cuttlefish bones are used to prepare highly porous hydroxyapatite (HA) scaffolds via hydrothermal treatment at 200 °C. Raw cuttlefish bones (CB) and the hydrothermal products have been analyzed and compared for their composition and microstructure, using X-ray powder diffraction (XRD), Optical Microscopy (OM), Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), porosity estimation and compressive strength measuring techniques. Characterization reveals that cuttlebone has high porosity approaching above 70%, and possesses the laminar structure of aragonite mixed with some organic materials. The compressive strength of the CB-HA is improved after coating with both polyvinyl alcohol (PVA) and polycaprolactone (PCL). Furthermore, our in vitro biocompatibility studies revealed that CB-HA and PVA coated CB-HA scaffolds are non-cytotoxic and support the adherence and proliferation of rMSCs, comparable to pure HA scaffolds. Altogether, our results suggest that naturally derived CB-HA, PVA and PCL coated CB-HA scaffolds are potential cheap candidates for bone tissue engineering applications, and also that PVA and PCL coatings provide better mechanical strength. © The Royal Society of Chemistry 2016.
AB - In the present study, cuttlefish bones are used to prepare highly porous hydroxyapatite (HA) scaffolds via hydrothermal treatment at 200 °C. Raw cuttlefish bones (CB) and the hydrothermal products have been analyzed and compared for their composition and microstructure, using X-ray powder diffraction (XRD), Optical Microscopy (OM), Scanning Electron Microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), porosity estimation and compressive strength measuring techniques. Characterization reveals that cuttlebone has high porosity approaching above 70%, and possesses the laminar structure of aragonite mixed with some organic materials. The compressive strength of the CB-HA is improved after coating with both polyvinyl alcohol (PVA) and polycaprolactone (PCL). Furthermore, our in vitro biocompatibility studies revealed that CB-HA and PVA coated CB-HA scaffolds are non-cytotoxic and support the adherence and proliferation of rMSCs, comparable to pure HA scaffolds. Altogether, our results suggest that naturally derived CB-HA, PVA and PCL coated CB-HA scaffolds are potential cheap candidates for bone tissue engineering applications, and also that PVA and PCL coatings provide better mechanical strength. © The Royal Society of Chemistry 2016.
KW - Biocompatibility
KW - Bone
KW - Cell culture
KW - Coatings
KW - Compressive strength
KW - Fourier transform infrared spectroscopy
KW - Hydroxyapatite
KW - Molluscs
KW - Polycaprolactone
KW - Porosity
KW - Scanning electron microscopy
KW - Shellfish
KW - Stem cells
KW - Tissue engineering
KW - X ray powder diffraction
KW - Bone tissue engineering
KW - Hydrothermal products
KW - Hydrothermal treatments
KW - Measuring technique
KW - Mesenchymal stem cell
KW - Poly (vinyl alcohol) (PVA)
KW - Porosity estimation
KW - Porous hydroxyapatite
KW - Scaffolds (biology)
U2 - 10.1039/c5ra22423c
DO - 10.1039/c5ra22423c
M3 - Journal article
VL - 6
SP - 32897
EP - 32904
JO - RSC Advances
JF - RSC Advances
SN - 2046-2069
IS - 39
ER -