Methane oxidation potential and preliminary analysis of methanotrophs in blanket bog peat using molecular ecology techniques

Biomedical and Life Sciences

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https://doi.org/10.1016/S0168-6496(96)00056-6
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Keywords

16S rRNA, methane oxidation potential, PCR, peat, phylogeny, soluble methane monooxygenase

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Methane oxidation potential and preliminary analysis of methanotrophs in blanket bog peat using molecular ecology techniques. / McDonald, Ian R.; Hall, Graham H.; Pickup, Roger W. et al.
In: FEMS Microbiology Ecology, Vol. 21, No. 3, 30.11.1996, p. 197-211.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{962c5222c9e04a7fa575a2e94e036f44,

title = "Methane oxidation potential and preliminary analysis of methanotrophs in blanket bog peat using molecular ecology techniques",

abstract = "The potential for methane oxidation was measured, and methanotroph gene sequences studied, in a peat core from the Moorhouse Nature Reserve, UK. Methane oxidation potential was observed in all depths of the peat core (down to 30 cm), and was inhibited by addition of acetylene, indicating the involvement of methane-oxidising bacteria. A peak of activity was shown in the 10-12 cm horizon, below which activity decreased with depth. Above this horizon, methane oxidation was relatively high and showed little change with depth. 16S rDNA libraries from several sections of the peat core were screened with methanotroph 16S rDNA probes designed to detect the genera Methylomonas, Methylococcus, Methylobacter and Methylosinus. Two clones, MHP14 and MHP17, hybridised strongly with the Methylosinus probe and upon complete sequencing and phylogenetic analysis were shown to group closely to the Methylosinus/Methylocystis genera of methanotrophs. However, the clones do form a distinct branch of their own, supported by BOOTSTRAP values, and may represent a novel group of acidophilic methanotrophs which have yet to be cultured.",

keywords = "16S rRNA, methane oxidation potential, PCR, peat, phylogeny, soluble methane monooxygenase",

author = "McDonald, {Ian R.} and Hall, {Graham H.} and Pickup, {Roger W.} and Murrell, {J. Colin}",

note = "Funding Information: We acknowledge the financial support provided by the Natural Environment Research Council through its Terrestrial Initiative in Global Environmental Researchp rogram (award GST/02/622).",

year = "1996",

month = nov,

day = "30",

doi = "10.1016/S0168-6496(96)00056-6",

language = "English",

volume = "21",

pages = "197--211",

journal = "FEMS Microbiology Ecology",

issn = "0168-6496",

publisher = "Wiley-Blackwell",

number = "3",

}

RIS

TY - JOUR

T1 - Methane oxidation potential and preliminary analysis of methanotrophs in blanket bog peat using molecular ecology techniques

AU - McDonald, Ian R.

AU - Hall, Graham H.

AU - Pickup, Roger W.

AU - Murrell, J. Colin

N1 - Funding Information: We acknowledge the financial support provided by the Natural Environment Research Council through its Terrestrial Initiative in Global Environmental Researchp rogram (award GST/02/622).

PY - 1996/11/30

Y1 - 1996/11/30

N2 - The potential for methane oxidation was measured, and methanotroph gene sequences studied, in a peat core from the Moorhouse Nature Reserve, UK. Methane oxidation potential was observed in all depths of the peat core (down to 30 cm), and was inhibited by addition of acetylene, indicating the involvement of methane-oxidising bacteria. A peak of activity was shown in the 10-12 cm horizon, below which activity decreased with depth. Above this horizon, methane oxidation was relatively high and showed little change with depth. 16S rDNA libraries from several sections of the peat core were screened with methanotroph 16S rDNA probes designed to detect the genera Methylomonas, Methylococcus, Methylobacter and Methylosinus. Two clones, MHP14 and MHP17, hybridised strongly with the Methylosinus probe and upon complete sequencing and phylogenetic analysis were shown to group closely to the Methylosinus/Methylocystis genera of methanotrophs. However, the clones do form a distinct branch of their own, supported by BOOTSTRAP values, and may represent a novel group of acidophilic methanotrophs which have yet to be cultured.

AB - The potential for methane oxidation was measured, and methanotroph gene sequences studied, in a peat core from the Moorhouse Nature Reserve, UK. Methane oxidation potential was observed in all depths of the peat core (down to 30 cm), and was inhibited by addition of acetylene, indicating the involvement of methane-oxidising bacteria. A peak of activity was shown in the 10-12 cm horizon, below which activity decreased with depth. Above this horizon, methane oxidation was relatively high and showed little change with depth. 16S rDNA libraries from several sections of the peat core were screened with methanotroph 16S rDNA probes designed to detect the genera Methylomonas, Methylococcus, Methylobacter and Methylosinus. Two clones, MHP14 and MHP17, hybridised strongly with the Methylosinus probe and upon complete sequencing and phylogenetic analysis were shown to group closely to the Methylosinus/Methylocystis genera of methanotrophs. However, the clones do form a distinct branch of their own, supported by BOOTSTRAP values, and may represent a novel group of acidophilic methanotrophs which have yet to be cultured.

KW - 16S rRNA

KW - methane oxidation potential

KW - PCR

KW - peat

KW - phylogeny

KW - soluble methane monooxygenase

U2 - 10.1016/S0168-6496(96)00056-6

DO - 10.1016/S0168-6496(96)00056-6

M3 - Journal article

AN - SCOPUS:0030298396

VL - 21

SP - 197

EP - 211

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 3

ER -

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