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Methods to assess subcellular compartments of muscle in C. elegans

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Methods to assess subcellular compartments of muscle in C. elegans. / Gaffney, Christopher J; Bass, Joseph J; Barratt, Thomas F et al.
In: Journal of Visualized Experiments, No. 93, 13.11.2014, p. e52043.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Gaffney, CJ, Bass, JJ, Barratt, TF & Szewczyk, NJ 2014, 'Methods to assess subcellular compartments of muscle in C. elegans', Journal of Visualized Experiments, no. 93, pp. e52043. https://doi.org/10.3791/52043

APA

Gaffney, C. J., Bass, J. J., Barratt, T. F., & Szewczyk, N. J. (2014). Methods to assess subcellular compartments of muscle in C. elegans. Journal of Visualized Experiments, (93), e52043. https://doi.org/10.3791/52043

Vancouver

Gaffney CJ, Bass JJ, Barratt TF, Szewczyk NJ. Methods to assess subcellular compartments of muscle in C. elegans. Journal of Visualized Experiments. 2014 Nov 13;(93):e52043. doi: 10.3791/52043

Author

Gaffney, Christopher J ; Bass, Joseph J ; Barratt, Thomas F et al. / Methods to assess subcellular compartments of muscle in C. elegans. In: Journal of Visualized Experiments. 2014 ; No. 93. pp. e52043.

Bibtex

@article{31df175a472b4f65867c473af22da040,
title = "Methods to assess subcellular compartments of muscle in C. elegans",
abstract = "Muscle is a dynamic tissue that responds to changes in nutrition, exercise, and disease state. The loss of muscle mass and function with disease and age are significant public health burdens. We currently understand little about the genetic regulation of muscle health with disease or age. The nematode C. elegans is an established model for understanding the genomic regulation of biological processes of interest. This worm's body wall muscles display a large degree of homology with the muscles of higher metazoan species. Since C. elegans is a transparent organism, the localization of GFP to mitochondria and sarcomeres allows visualization of these structures in vivo. Similarly, feeding animals cationic dyes, which accumulate based on the existence of a mitochondrial membrane potential, allows the assessment of mitochondrial function in vivo. These methods, as well as assessment of muscle protein homeostasis, are combined with assessment of whole animal muscle function, in the form of movement assays, to allow correlation of sub-cellular defects with functional measures of muscle performance. Thus, C. elegans provides a powerful platform with which to assess the impact of mutations, gene knockdown, and/or chemical compounds upon muscle structure and function. Lastly, as GFP, cationic dyes, and movement assays are assessed non-invasively, prospective studies of muscle structure and function can be conducted across the whole life course and this at present cannot be easily investigated in vivo in any other organism.",
keywords = "Animals, Caenorhabditis elegans, Green Fluorescent Proteins, Muscle, Skeletal, Structure-Activity Relationship, Subcellular Fractions, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Video-Audio Media",
author = "Gaffney, {Christopher J} and Bass, {Joseph J} and Barratt, {Thomas F} and Szewczyk, {Nathaniel J}",
year = "2014",
month = nov,
day = "13",
doi = "10.3791/52043",
language = "English",
pages = "e52043",
journal = "Journal of Visualized Experiments",
issn = "1940-087X",
publisher = "MYJoVE Corporation",
number = "93",

}

RIS

TY - JOUR

T1 - Methods to assess subcellular compartments of muscle in C. elegans

AU - Gaffney, Christopher J

AU - Bass, Joseph J

AU - Barratt, Thomas F

AU - Szewczyk, Nathaniel J

PY - 2014/11/13

Y1 - 2014/11/13

N2 - Muscle is a dynamic tissue that responds to changes in nutrition, exercise, and disease state. The loss of muscle mass and function with disease and age are significant public health burdens. We currently understand little about the genetic regulation of muscle health with disease or age. The nematode C. elegans is an established model for understanding the genomic regulation of biological processes of interest. This worm's body wall muscles display a large degree of homology with the muscles of higher metazoan species. Since C. elegans is a transparent organism, the localization of GFP to mitochondria and sarcomeres allows visualization of these structures in vivo. Similarly, feeding animals cationic dyes, which accumulate based on the existence of a mitochondrial membrane potential, allows the assessment of mitochondrial function in vivo. These methods, as well as assessment of muscle protein homeostasis, are combined with assessment of whole animal muscle function, in the form of movement assays, to allow correlation of sub-cellular defects with functional measures of muscle performance. Thus, C. elegans provides a powerful platform with which to assess the impact of mutations, gene knockdown, and/or chemical compounds upon muscle structure and function. Lastly, as GFP, cationic dyes, and movement assays are assessed non-invasively, prospective studies of muscle structure and function can be conducted across the whole life course and this at present cannot be easily investigated in vivo in any other organism.

AB - Muscle is a dynamic tissue that responds to changes in nutrition, exercise, and disease state. The loss of muscle mass and function with disease and age are significant public health burdens. We currently understand little about the genetic regulation of muscle health with disease or age. The nematode C. elegans is an established model for understanding the genomic regulation of biological processes of interest. This worm's body wall muscles display a large degree of homology with the muscles of higher metazoan species. Since C. elegans is a transparent organism, the localization of GFP to mitochondria and sarcomeres allows visualization of these structures in vivo. Similarly, feeding animals cationic dyes, which accumulate based on the existence of a mitochondrial membrane potential, allows the assessment of mitochondrial function in vivo. These methods, as well as assessment of muscle protein homeostasis, are combined with assessment of whole animal muscle function, in the form of movement assays, to allow correlation of sub-cellular defects with functional measures of muscle performance. Thus, C. elegans provides a powerful platform with which to assess the impact of mutations, gene knockdown, and/or chemical compounds upon muscle structure and function. Lastly, as GFP, cationic dyes, and movement assays are assessed non-invasively, prospective studies of muscle structure and function can be conducted across the whole life course and this at present cannot be easily investigated in vivo in any other organism.

KW - Animals

KW - Caenorhabditis elegans

KW - Green Fluorescent Proteins

KW - Muscle, Skeletal

KW - Structure-Activity Relationship

KW - Subcellular Fractions

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

KW - Video-Audio Media

U2 - 10.3791/52043

DO - 10.3791/52043

M3 - Journal article

C2 - 25489753

SP - e52043

JO - Journal of Visualized Experiments

JF - Journal of Visualized Experiments

SN - 1940-087X

IS - 93

ER -