Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Milling plant and soil material in plastic tubes over-estimates carbon and under-estimates nitrogen concentrations
AU - Smith, Stuart W.
AU - Robertson, A. H. Jean
AU - Meharg, Andrew A.
AU - Pakeman, Robin J.
AU - Johnson, David
AU - Woodin, Sarah J.
AU - van der Wal, Rene
PY - 2013/8/31
Y1 - 2013/8/31
N2 - Background and aimsMilling of plant and soil material in plastic tubes, such as microcentrifuge tubes, over-estimates carbon (C) and under-estimates nitrogen (N) concentrations due to the introduction of polypropylene into milled samples, as identified using Fourier-transform infra-red spectroscopy.Methods and resultsThis study compares C and N concentrations of roots and soil milled in microcentrifuge tubes versus stainless steel containers, demonstrating that a longer milling time, greater milling intensity, smaller sample size and inclusion of abrasive sample material all increase polypropylene contamination from plastic tubes leading to overestimation of C concentrations by up to 8 % (0.08 g g−1).ConclusionsErroneous estimations of C and N, and other analytes, must be assumed after milling in plastic tubes and milling methods should be adapted to minimise such error.
AB - Background and aimsMilling of plant and soil material in plastic tubes, such as microcentrifuge tubes, over-estimates carbon (C) and under-estimates nitrogen (N) concentrations due to the introduction of polypropylene into milled samples, as identified using Fourier-transform infra-red spectroscopy.Methods and resultsThis study compares C and N concentrations of roots and soil milled in microcentrifuge tubes versus stainless steel containers, demonstrating that a longer milling time, greater milling intensity, smaller sample size and inclusion of abrasive sample material all increase polypropylene contamination from plastic tubes leading to overestimation of C concentrations by up to 8 % (0.08 g g−1).ConclusionsErroneous estimations of C and N, and other analytes, must be assumed after milling in plastic tubes and milling methods should be adapted to minimise such error.
KW - Carbon
KW - Fourier-transform infrared spectroscopy
KW - Grinding
KW - Microcentrifuge tubes
KW - Milling
KW - Nitrogen
U2 - 10.1007/s11104-013-1599-y
DO - 10.1007/s11104-013-1599-y
M3 - Journal article
VL - 369
SP - 509
EP - 513
JO - Plant and Soil
JF - Plant and Soil
SN - 0032-079X
IS - 1-2
ER -