Rights statement: This is the author’s version of a work that was accepted for publication in Applied Soil Ecology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Applied Soil Ecology, 125, 2018 DOI: 10.1016/j.apsoil.2017.12.010
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Mineralisation of 14C-phenanthrene in PAH-diesel contaminated soil
T2 - Impact of Sorghum bicolor and Medicago sativa mono- or mixed culture
AU - Umeh, Anthony C.
AU - Vazquez Cuevas, Gabriela
AU - Semple, Kirk Taylor
N1 - This is the author’s version of a work that was accepted for publication in Applied Soil Ecology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Applied Soil Ecology, 125, 2018 DOI: 10.1016/j.apsoil.2017.12.010
PY - 2018/4
Y1 - 2018/4
N2 - Plant-assisted biodegradation can offer a cost-effective and sustainable approach for the bioremediation of PAHs in soil. As such, selecting the most appropriate plant species is important. The potential for plant-assisted biodegradation of complex PAH-diesel mixtures in soil by sorghum (Sorghum bicolor) and alfalfa (Medicago sativa) grown as monocultures and mixed cultures using 14C-contaminants has not been widely reported. The objective of this study was to assess 14C-phenanthrene mineralisation profiles in mixtures of PAH-diesel in soil in the presence of Sorghum bicolor and Medicago sativa. Soil was spiked with PAHs and diesel, after which M. sativa and S. bicolor were introduced and grown as mono- or mixed- cultures. The toxicity of the PAH-diesel oil mixture in the planted treatments, as well as its effect on the mineralisation of 14C-phenanthrene were evaluated. Monocultures of both plant species tolerated the complex PAH-diesel mixtures based on growth and survival, and increased rates and extents of 14C-phenanthrene mineralisation in soil. The influence of PAH concentration on 14C-phenanthrene mineralisation profiles varied in planted and unplanted treatments. The rates and extents of 14C-phenanthrene mineralisation tended to decrease in diesel amended soil, especially at low PAH concentrations. To the best of the authors’ knowledge, this is the first report of 14C-phenanthrene mineralisation patterns in complex PAH-diesel oil mixtures contaminated soil especially with respect to the specified plant species. The findings offer new insights on mono- and multi-species phytotoxicity as well as plant-assisted biodegradation of PAH mixtures in soil which may be useful in the risk assessment and remediation of contaminated sites.
AB - Plant-assisted biodegradation can offer a cost-effective and sustainable approach for the bioremediation of PAHs in soil. As such, selecting the most appropriate plant species is important. The potential for plant-assisted biodegradation of complex PAH-diesel mixtures in soil by sorghum (Sorghum bicolor) and alfalfa (Medicago sativa) grown as monocultures and mixed cultures using 14C-contaminants has not been widely reported. The objective of this study was to assess 14C-phenanthrene mineralisation profiles in mixtures of PAH-diesel in soil in the presence of Sorghum bicolor and Medicago sativa. Soil was spiked with PAHs and diesel, after which M. sativa and S. bicolor were introduced and grown as mono- or mixed- cultures. The toxicity of the PAH-diesel oil mixture in the planted treatments, as well as its effect on the mineralisation of 14C-phenanthrene were evaluated. Monocultures of both plant species tolerated the complex PAH-diesel mixtures based on growth and survival, and increased rates and extents of 14C-phenanthrene mineralisation in soil. The influence of PAH concentration on 14C-phenanthrene mineralisation profiles varied in planted and unplanted treatments. The rates and extents of 14C-phenanthrene mineralisation tended to decrease in diesel amended soil, especially at low PAH concentrations. To the best of the authors’ knowledge, this is the first report of 14C-phenanthrene mineralisation patterns in complex PAH-diesel oil mixtures contaminated soil especially with respect to the specified plant species. The findings offer new insights on mono- and multi-species phytotoxicity as well as plant-assisted biodegradation of PAH mixtures in soil which may be useful in the risk assessment and remediation of contaminated sites.
KW - PAH mixtures
KW - Diesel oil amendment
KW - Phytotoxicity
KW - Sorghum bicolor
KW - Medicago sativa
KW - 14C-phenanthrene mineralisation
U2 - 10.1016/j.apsoil.2017.12.010
DO - 10.1016/j.apsoil.2017.12.010
M3 - Journal article
VL - 125
SP - 46
EP - 55
JO - Applied Soil Ecology
JF - Applied Soil Ecology
SN - 0929-1393
ER -