TY - JOUR

T1 - Miniaturised free flow isotachophoresis of bacteria using an injection moulded separation device

AU - Prest, Jeff E.

AU - Baldock, Sara J.

AU - Fielden, Peter R.

AU - Goddard, Nicholas J.

AU - Goodacre, Royston

AU - O'Connor, Richard

AU - Brown, Bernard J. Treves

PY - 2012/8/15

Y1 - 2012/8/15

N2 - A new design of miniaturised free flow electrophoresis device has been produced. The design contains a separation chamber that is 45 mm long by 31.7 mm wide with a depth of 50 μm and has nine inlet and nine outlet holes to allow for fraction collection. The devices were formed of polystyrene with carbon fibre loaded polystyrene drive electrodes and produced using injection moulding. This means that the devices are low cost and can potentially be mass produced. The devices were used for free flow isotachophoresis (FFITP), a technique that can be used for focussing and concentrating analytes contained within complex sample matrices. The operation of the devices was demonstrated by performing separations of dyes and bacterial samples. Analysis of the output from FFITP separations of samples containing the bacterium Erwinia herbicola, a biological pathogen, by cell culturing and counting showed that fractionation of the output was achieved.

AB - A new design of miniaturised free flow electrophoresis device has been produced. The design contains a separation chamber that is 45 mm long by 31.7 mm wide with a depth of 50 μm and has nine inlet and nine outlet holes to allow for fraction collection. The devices were formed of polystyrene with carbon fibre loaded polystyrene drive electrodes and produced using injection moulding. This means that the devices are low cost and can potentially be mass produced. The devices were used for free flow isotachophoresis (FFITP), a technique that can be used for focussing and concentrating analytes contained within complex sample matrices. The operation of the devices was demonstrated by performing separations of dyes and bacterial samples. Analysis of the output from FFITP separations of samples containing the bacterium Erwinia herbicola, a biological pathogen, by cell culturing and counting showed that fractionation of the output was achieved.

KW - Free flow isotachophoresis

KW - Free flow electrophoresis

KW - Miniaturisation

KW - Sample preparation

KW - Bacteria

U2 - 10.1016/j.jchromb.2012.06.040

DO - 10.1016/j.jchromb.2012.06.040

M3 - Journal article

VL - 903

SP - 53

EP - 59

JO - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

ER -