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Miniaturised isotachophoresis of DNA

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Miniaturised isotachophoresis of DNA. / Prest, Jeff E.; Baldock, Sara J.; Day, Philip J. R.; Fielden, Peter R.; Goddard, Nicholas J.; Brown, Bernard J. Treves.

In: Journal of Chromatography A, Vol. 1156, No. 1-2, 13.07.2007, p. 154-159.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Prest, JE, Baldock, SJ, Day, PJR, Fielden, PR, Goddard, NJ & Brown, BJT 2007, 'Miniaturised isotachophoresis of DNA', Journal of Chromatography A, vol. 1156, no. 1-2, pp. 154-159. https://doi.org/10.1016/j.chroma.2007.01.026

APA

Prest, J. E., Baldock, S. J., Day, P. J. R., Fielden, P. R., Goddard, N. J., & Brown, B. J. T. (2007). Miniaturised isotachophoresis of DNA. Journal of Chromatography A, 1156(1-2), 154-159. https://doi.org/10.1016/j.chroma.2007.01.026

Vancouver

Prest JE, Baldock SJ, Day PJR, Fielden PR, Goddard NJ, Brown BJT. Miniaturised isotachophoresis of DNA. Journal of Chromatography A. 2007 Jul 13;1156(1-2):154-159. https://doi.org/10.1016/j.chroma.2007.01.026

Author

Prest, Jeff E. ; Baldock, Sara J. ; Day, Philip J. R. ; Fielden, Peter R. ; Goddard, Nicholas J. ; Brown, Bernard J. Treves. / Miniaturised isotachophoresis of DNA. In: Journal of Chromatography A. 2007 ; Vol. 1156, No. 1-2. pp. 154-159.

Bibtex

@article{3312d6c5deea4da280f9a33d406d8ff9,
title = "Miniaturised isotachophoresis of DNA",
abstract = "This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5 mM perchloric acid at pH 6.0 and a terminating electrolyte of 10 mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities.",
keywords = "DNA, Isotachophoresis, Sample preparation , Miniaturisation, Microdevice, Chip",
author = "Prest, {Jeff E.} and Baldock, {Sara J.} and Day, {Philip J. R.} and Fielden, {Peter R.} and Goddard, {Nicholas J.} and Brown, {Bernard J. Treves}",
year = "2007",
month = jul,
day = "13",
doi = "10.1016/j.chroma.2007.01.026",
language = "English",
volume = "1156",
pages = "154--159",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Miniaturised isotachophoresis of DNA

AU - Prest, Jeff E.

AU - Baldock, Sara J.

AU - Day, Philip J. R.

AU - Fielden, Peter R.

AU - Goddard, Nicholas J.

AU - Brown, Bernard J. Treves

PY - 2007/7/13

Y1 - 2007/7/13

N2 - This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5 mM perchloric acid at pH 6.0 and a terminating electrolyte of 10 mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities.

AB - This paper presents the findings of a feasibility study investigating the behaviour of DNA under conditions of miniaturised isotachophoresis. An electrolyte system comprising a leading electrolyte of 5 mM perchloric acid at pH 6.0 and a terminating electrolyte of 10 mM gallic acid was devised and used to perform isotachophoresis of DNA containing samples on a miniaturised poly(methyl methacrylate) device. Under such conditions it was found that no separation of DNA fragments was observed with the substance migrating instead as a single isotachophoretic zone. Whilst such a result shows the method is unsuitable for analysis DNA it offers significant potential as a means of sample preparation for subsequent analysis using another method. This is because the single zone of DNA formed is preconcentrated to a constant concentration governed by the leading ion and is separated from all species with different effective electrophoretic mobilities.

KW - DNA

KW - Isotachophoresis

KW - Sample preparation

KW - Miniaturisation

KW - Microdevice

KW - Chip

U2 - 10.1016/j.chroma.2007.01.026

DO - 10.1016/j.chroma.2007.01.026

M3 - Journal article

VL - 1156

SP - 154

EP - 159

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -