Home > Research > Publications & Outputs > Mitochondrial DNA profiling reveals two lineage...

Electronic data

  • sunbears_accepted

    Rights statement: This is a pre-copy-editing, author-produced PDF of an article accepted for publication inThe Journal of Heredity following peer review. The definitive publisher-authenticated version Wai-Ling Lai, Jactty Chew, Derek Gatherer, Dusit Ngoprasert, Sadequr Rahman, Qasim Ayub, Adrian Kannan, Eleanor Vaughan, Siew Te Wong, Noor Azleen Mohd Kulaimi, Shyamala Ratnayeke, Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia, Journal of Heredity, , esab004, https://doi.org/10.1093/jhered/esab004 is available online at: add url

    Accepted author manuscript, 0.99 MB, PDF document

    Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License

Text available via DOI:

View graph of relations

Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia. / Lai, Wai-Ling; Chew, Jactty; Gatherer, Derek; Ngoprasert, Dusit; Rahman, Sadequr; Ayub, Qasim; Kannan, Adrian; Vaughan, Eleanor; Wong, Siew Te; Kulaimi, Noor Azleen Mohd; Ratnayeke, Shyamala.

In: The Journal of heredity, Vol. 112, No. 2, 30.03.2021, p. 214–220.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Lai, W-L, Chew, J, Gatherer, D, Ngoprasert, D, Rahman, S, Ayub, Q, Kannan, A, Vaughan, E, Wong, ST, Kulaimi, NAM & Ratnayeke, S 2021, 'Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia', The Journal of heredity, vol. 112, no. 2, pp. 214–220. https://doi.org/10.1093/jhered/esab004

APA

Lai, W-L., Chew, J., Gatherer, D., Ngoprasert, D., Rahman, S., Ayub, Q., Kannan, A., Vaughan, E., Wong, S. T., Kulaimi, N. A. M., & Ratnayeke, S. (2021). Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia. The Journal of heredity, 112(2), 214–220. https://doi.org/10.1093/jhered/esab004

Vancouver

Lai W-L, Chew J, Gatherer D, Ngoprasert D, Rahman S, Ayub Q et al. Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia. The Journal of heredity. 2021 Mar 30;112(2):214–220. https://doi.org/10.1093/jhered/esab004

Author

Lai, Wai-Ling ; Chew, Jactty ; Gatherer, Derek ; Ngoprasert, Dusit ; Rahman, Sadequr ; Ayub, Qasim ; Kannan, Adrian ; Vaughan, Eleanor ; Wong, Siew Te ; Kulaimi, Noor Azleen Mohd ; Ratnayeke, Shyamala. / Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia. In: The Journal of heredity. 2021 ; Vol. 112, No. 2. pp. 214–220.

Bibtex

@article{1d5b901ef7d142bfad942548dffd4bca,
title = "Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia",
abstract = "Sun bear populations are fragmented and at risk from habitat loss and exploitation for body parts. These threats are made worse by significant gaps in knowledge of sun bear population genetic diversity, population connectivity, and taxonomically significant management units. Using a complete sun bear mitochondrial genome, we developed a set of mitochondrial markers to assess haplotype variation and the evolutionary history of sun bears from Peninsular (West) Malaysia, and Sabah (East Malaysia). Genetic samples from 28 sun bears from Peninsular Malaysia, 36 from Sabah, and 18 from Thailand were amplified with primers targeting a 1,800 bp region of the mitochondrial genome including the complete mitochondrial control region and adjacent genes. Sequences were analyzed using phylogenetic methods. We identified 51 mitochondrial haplotypes among 82 sun bears. Phylogenetic and network analyses provided strong support for a deep split between Malaysian sun bears and sun bears in East Thailand and Yunnan province in China. The Malaysian lineage was further subdivided into two clades: Peninsular Malaysian and Malaysian Borneo (Sabah). Sun bears from Thailand occurred in both Sabah and Peninsular Malaysian clades. Our study supports recent findings that sun bears from Sundaland form a distinct clade from those in China and Indochina with Thailand possessing lineages from the three clades. Importantly we demonstrate a more recent and clear genetic delineation between sun bears from the Malay Peninsula and Sabah indicating historical barriers to gene flow within the Sundaic region.",
keywords = "sunbear, Helarctos malayanus, phylogenetics, ursidae, mitochondria, phylogeography, phylodynamics",
author = "Wai-Ling Lai and Jactty Chew and Derek Gatherer and Dusit Ngoprasert and Sadequr Rahman and Qasim Ayub and Adrian Kannan and Eleanor Vaughan and Wong, {Siew Te} and Kulaimi, {Noor Azleen Mohd} and Shyamala Ratnayeke",
note = "This is a pre-copy-editing, author-produced PDF of an article accepted for publication inThe Journal of Heredity following peer review. The definitive publisher-authenticated version Wai-Ling Lai, Jactty Chew, Derek Gatherer, Dusit Ngoprasert, Sadequr Rahman, Qasim Ayub, Adrian Kannan, Eleanor Vaughan, Siew Te Wong, Noor Azleen Mohd Kulaimi, Shyamala Ratnayeke, Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia, Journal of Heredity, , esab004, https://doi.org/10.1093/jhered/esab004 is available online at: add url",
year = "2021",
month = mar,
day = "30",
doi = "10.1093/jhered/esab004",
language = "English",
volume = "112",
pages = "214–220",
journal = "The Journal of heredity",
issn = "0022-1503",
publisher = "Oxford University Press",
number = "2",

}

RIS

TY - JOUR

T1 - Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia

AU - Lai, Wai-Ling

AU - Chew, Jactty

AU - Gatherer, Derek

AU - Ngoprasert, Dusit

AU - Rahman, Sadequr

AU - Ayub, Qasim

AU - Kannan, Adrian

AU - Vaughan, Eleanor

AU - Wong, Siew Te

AU - Kulaimi, Noor Azleen Mohd

AU - Ratnayeke, Shyamala

N1 - This is a pre-copy-editing, author-produced PDF of an article accepted for publication inThe Journal of Heredity following peer review. The definitive publisher-authenticated version Wai-Ling Lai, Jactty Chew, Derek Gatherer, Dusit Ngoprasert, Sadequr Rahman, Qasim Ayub, Adrian Kannan, Eleanor Vaughan, Siew Te Wong, Noor Azleen Mohd Kulaimi, Shyamala Ratnayeke, Mitochondrial DNA profiling reveals two lineages of sun bears in East and West Malaysia, Journal of Heredity, , esab004, https://doi.org/10.1093/jhered/esab004 is available online at: add url

PY - 2021/3/30

Y1 - 2021/3/30

N2 - Sun bear populations are fragmented and at risk from habitat loss and exploitation for body parts. These threats are made worse by significant gaps in knowledge of sun bear population genetic diversity, population connectivity, and taxonomically significant management units. Using a complete sun bear mitochondrial genome, we developed a set of mitochondrial markers to assess haplotype variation and the evolutionary history of sun bears from Peninsular (West) Malaysia, and Sabah (East Malaysia). Genetic samples from 28 sun bears from Peninsular Malaysia, 36 from Sabah, and 18 from Thailand were amplified with primers targeting a 1,800 bp region of the mitochondrial genome including the complete mitochondrial control region and adjacent genes. Sequences were analyzed using phylogenetic methods. We identified 51 mitochondrial haplotypes among 82 sun bears. Phylogenetic and network analyses provided strong support for a deep split between Malaysian sun bears and sun bears in East Thailand and Yunnan province in China. The Malaysian lineage was further subdivided into two clades: Peninsular Malaysian and Malaysian Borneo (Sabah). Sun bears from Thailand occurred in both Sabah and Peninsular Malaysian clades. Our study supports recent findings that sun bears from Sundaland form a distinct clade from those in China and Indochina with Thailand possessing lineages from the three clades. Importantly we demonstrate a more recent and clear genetic delineation between sun bears from the Malay Peninsula and Sabah indicating historical barriers to gene flow within the Sundaic region.

AB - Sun bear populations are fragmented and at risk from habitat loss and exploitation for body parts. These threats are made worse by significant gaps in knowledge of sun bear population genetic diversity, population connectivity, and taxonomically significant management units. Using a complete sun bear mitochondrial genome, we developed a set of mitochondrial markers to assess haplotype variation and the evolutionary history of sun bears from Peninsular (West) Malaysia, and Sabah (East Malaysia). Genetic samples from 28 sun bears from Peninsular Malaysia, 36 from Sabah, and 18 from Thailand were amplified with primers targeting a 1,800 bp region of the mitochondrial genome including the complete mitochondrial control region and adjacent genes. Sequences were analyzed using phylogenetic methods. We identified 51 mitochondrial haplotypes among 82 sun bears. Phylogenetic and network analyses provided strong support for a deep split between Malaysian sun bears and sun bears in East Thailand and Yunnan province in China. The Malaysian lineage was further subdivided into two clades: Peninsular Malaysian and Malaysian Borneo (Sabah). Sun bears from Thailand occurred in both Sabah and Peninsular Malaysian clades. Our study supports recent findings that sun bears from Sundaland form a distinct clade from those in China and Indochina with Thailand possessing lineages from the three clades. Importantly we demonstrate a more recent and clear genetic delineation between sun bears from the Malay Peninsula and Sabah indicating historical barriers to gene flow within the Sundaic region.

KW - sunbear

KW - Helarctos malayanus

KW - phylogenetics

KW - ursidae

KW - mitochondria

KW - phylogeography

KW - phylodynamics

U2 - 10.1093/jhered/esab004

DO - 10.1093/jhered/esab004

M3 - Journal article

C2 - 33439997

VL - 112

SP - 214

EP - 220

JO - The Journal of heredity

JF - The Journal of heredity

SN - 0022-1503

IS - 2

ER -