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Licence: CC BY: Creative Commons Attribution 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Molecular basis for PrimPol recruitment to replication forks by RPA
AU - Guilliam, Thomas, A.
AU - Brissett, Nigel, C.
AU - Ehlinger, Aaron
AU - Keen, Benjamin, A.
AU - Kolesar, Peter
AU - Taylor, Elaine Moira
AU - Bailey, Laura
AU - Lindsay, Howard David
AU - Chazin, Walter J.
AU - Doherty, Aidan, J.
PY - 2017/5/23
Y1 - 2017/5/23
N2 - DNA damage and secondary structures can act as potent obstacles to the replication machinery. Persistent stalling events lead to genomic instability and therefore numerous cellular tolerance mechanisms exist to complete genome duplication in the presence of such impediments. In addition to translesion synthesis (TLS) polymerases, eukaryotic cells contain a multi-functionalreplicative enzyme called Primase-Polymerase (PrimPol) that is capable of directly bypassing DNA damage by TLS, as well as repriming replication restart downstream of lesions and secondary structures. Here, we report that PrimPol is recruited to re-prime stalled replication through its interaction with RPA. Using crystallographic and biophysical approaches, we identify that PrimPolpossesses two RPA-interacting motifs and identify the key residues required for these interactions. We demonstrate that one of these motifs is critical for PrimPol’s recruitment to stalled replication forks in vivo thus facilitating its role in re-priming DNA synthesis. In addition, biochemical analysis reveals that RPA serves to stimulate the primase activity of PrimPol. Together, these findingsprovide unprecedented molecular insights into PrimPol’s mode of recruitment to stalled forks that enables it to efficiently re-prime the restart of DNA replication.
AB - DNA damage and secondary structures can act as potent obstacles to the replication machinery. Persistent stalling events lead to genomic instability and therefore numerous cellular tolerance mechanisms exist to complete genome duplication in the presence of such impediments. In addition to translesion synthesis (TLS) polymerases, eukaryotic cells contain a multi-functionalreplicative enzyme called Primase-Polymerase (PrimPol) that is capable of directly bypassing DNA damage by TLS, as well as repriming replication restart downstream of lesions and secondary structures. Here, we report that PrimPol is recruited to re-prime stalled replication through its interaction with RPA. Using crystallographic and biophysical approaches, we identify that PrimPolpossesses two RPA-interacting motifs and identify the key residues required for these interactions. We demonstrate that one of these motifs is critical for PrimPol’s recruitment to stalled replication forks in vivo thus facilitating its role in re-priming DNA synthesis. In addition, biochemical analysis reveals that RPA serves to stimulate the primase activity of PrimPol. Together, these findingsprovide unprecedented molecular insights into PrimPol’s mode of recruitment to stalled forks that enables it to efficiently re-prime the restart of DNA replication.
U2 - 10.1038/NCOMMS15222
DO - 10.1038/NCOMMS15222
M3 - Journal article
VL - 8
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 15222
ER -