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Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape

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Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape. / Andre, Jane; King, R. Andrew; Sturzenbaum, Stephen R. et al.
In: Environmental Pollution, Vol. 158, No. 3, 03.2010, p. 883-890.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Andre, J, King, RA, Sturzenbaum, SR, Kille, P, Hodson, ME & Morgan, AJ 2010, 'Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape', Environmental Pollution, vol. 158, no. 3, pp. 883-890. https://doi.org/10.1016/j.envpol.2009.09.021

APA

Andre, J., King, R. A., Sturzenbaum, S. R., Kille, P., Hodson, M. E., & Morgan, A. J. (2010). Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape. Environmental Pollution, 158(3), 883-890. https://doi.org/10.1016/j.envpol.2009.09.021

Vancouver

Andre J, King RA, Sturzenbaum SR, Kille P, Hodson ME, Morgan AJ. Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape. Environmental Pollution. 2010 Mar;158(3):883-890. doi: 10.1016/j.envpol.2009.09.021

Author

Andre, Jane ; King, R. Andrew ; Sturzenbaum, Stephen R. et al. / Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape. In: Environmental Pollution. 2010 ; Vol. 158, No. 3. pp. 883-890.

Bibtex

@article{47400d32f57f43a18b511db057e33eb2,
title = "Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape",
abstract = "A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages ({\textquoteleft}A{\textquoteright} and {\textquoteleft}B{\textquoteright}), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central {\textquoteleft}ecological island{\textquoteright} site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies.",
keywords = "Ecotoxicology, Pb & Ca , Genotyping, FTIR , cDNA-libraries (SERCA)",
author = "Jane Andre and King, {R. Andrew} and Sturzenbaum, {Stephen R.} and Peter Kille and Hodson, {Mark E.} and Morgan, {A. John}",
year = "2010",
month = mar,
doi = "10.1016/j.envpol.2009.09.021",
language = "English",
volume = "158",
pages = "883--890",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "Elsevier Ltd",
number = "3",

}

RIS

TY - JOUR

T1 - Molecular genetic differentiation in earthworms inhabiting a heterogenous Pb-polluted landscape

AU - Andre, Jane

AU - King, R. Andrew

AU - Sturzenbaum, Stephen R.

AU - Kille, Peter

AU - Hodson, Mark E.

AU - Morgan, A. John

PY - 2010/3

Y1 - 2010/3

N2 - A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages (‘A’ and ‘B’), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central ‘ecological island’ site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies.

AB - A Pb-mine site situated on acidic soil, but comprising of Ca-enriched islands around derelict buildings was used to study the spatial pattern of genetic diversity in Lumbricus rubellus. Two distinct genetic lineages (‘A’ and ‘B’), differentiated at both the mitochondrial (mtDNA COII) and nuclear level (AFLPs) were revealed with a mean inter-lineage mtDNA sequence divergence of approximately 13%, indicative of a cryptic species complex. AFLP analysis indicates that lineage A individuals within one central ‘ecological island’ site are uniquely clustered, with little genetic overlap with lineage A individuals at the two peripheral sites. FTIR microspectroscopy of Pb-sequestering chloragocytes revealed different phosphate profiles in residents of adjacent acidic and calcareous islands. Bioinformatics found over-representation of Ca pathway genes in ESTPb libraries. Subsequent sequencing of a Ca-transport gene, SERCA, revealed mutations in the protein's cytosolic domain. We recommend the mandatory genotyping of all individuals prior to field-based ecotoxicological assays, particularly those using discriminating genomic technologies.

KW - Ecotoxicology

KW - Pb & Ca

KW - Genotyping

KW - FTIR

KW - cDNA-libraries (SERCA)

U2 - 10.1016/j.envpol.2009.09.021

DO - 10.1016/j.envpol.2009.09.021

M3 - Journal article

VL - 158

SP - 883

EP - 890

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

IS - 3

ER -