Molecular profiling of soil animal diversity in natural ecosystems : incongruence of molecular and morphological results.

Lancaster Environment Centre

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https://doi.org/10.1016/j.soilbio.2009.02.003
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Keywords

Biodiversity, Soil, DNA sequence, Community structure, Phylogenetic analysis, Morphological analysis

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Molecular profiling of soil animal diversity in natural ecosystems : incongruence of molecular and morphological results. / Wu, Tiehang H.; Ayres, Edward; Li, Grace et al.
In: Soil Biology and Biochemistry, Vol. 41, No. 4, 04.2009, p. 849-857.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Author

Wu, Tiehang H. ; Ayres, Edward ; Li, Grace et al. / Molecular profiling of soil animal diversity in natural ecosystems : incongruence of molecular and morphological results. In: Soil Biology and Biochemistry. 2009 ; Vol. 41, No. 4. pp. 849-857.

Bibtex

@article{8a64f197e4d140cfa4c9072d6ec38a0d,

title = "Molecular profiling of soil animal diversity in natural ecosystems : incongruence of molecular and morphological results.",

abstract = "A major problem facing ecologists is obtaining a complete picture of the highly complex soil community. While DNA-based methods are routinely used to assess prokaryote community structure and diversity in soil, approaches for measuring the total faunal community are not yet available. This is due to difficulties such as designing primers specific to a range of soil animals while excluding other eukaryotes. Instead, scientists use laborious and specialized taxonomic methods for extracting and identifying soil fauna. We examined this problem using DNA sequencing to profile soil animal diversity across two Alaskan ecosystems and compare the results with morphological analyses. Of 5267 sequences, representing 549 operational taxonomic units (OTU), only 18 OTUs were common to both sites. Representatives included 8 phyla, dominated by arthropods and nematodes. This is the most comprehensive molecular analysis of soil fauna to date, and provides a tool to rapidly assess a missing component of soil biodiversity.",

keywords = "Biodiversity, Soil, DNA sequence, Community structure, Phylogenetic analysis, Morphological analysis",

author = "Wu, {Tiehang H.} and Edward Ayres and Grace Li and Bardgett, {Richard D.} and Wall, {Diana H.} and Garey, {James R.}",

year = "2009",

month = apr,

doi = "10.1016/j.soilbio.2009.02.003",

language = "English",

volume = "41",

pages = "849--857",

journal = "Soil Biology and Biochemistry",

issn = "0038-0717",

publisher = "Elsevier Ltd",

number = "4",

}

RIS

TY - JOUR

T1 - Molecular profiling of soil animal diversity in natural ecosystems : incongruence of molecular and morphological results.

AU - Wu, Tiehang H.

AU - Ayres, Edward

AU - Li, Grace

AU - Bardgett, Richard D.

AU - Wall, Diana H.

AU - Garey, James R.

PY - 2009/4

Y1 - 2009/4

N2 - A major problem facing ecologists is obtaining a complete picture of the highly complex soil community. While DNA-based methods are routinely used to assess prokaryote community structure and diversity in soil, approaches for measuring the total faunal community are not yet available. This is due to difficulties such as designing primers specific to a range of soil animals while excluding other eukaryotes. Instead, scientists use laborious and specialized taxonomic methods for extracting and identifying soil fauna. We examined this problem using DNA sequencing to profile soil animal diversity across two Alaskan ecosystems and compare the results with morphological analyses. Of 5267 sequences, representing 549 operational taxonomic units (OTU), only 18 OTUs were common to both sites. Representatives included 8 phyla, dominated by arthropods and nematodes. This is the most comprehensive molecular analysis of soil fauna to date, and provides a tool to rapidly assess a missing component of soil biodiversity.

AB - A major problem facing ecologists is obtaining a complete picture of the highly complex soil community. While DNA-based methods are routinely used to assess prokaryote community structure and diversity in soil, approaches for measuring the total faunal community are not yet available. This is due to difficulties such as designing primers specific to a range of soil animals while excluding other eukaryotes. Instead, scientists use laborious and specialized taxonomic methods for extracting and identifying soil fauna. We examined this problem using DNA sequencing to profile soil animal diversity across two Alaskan ecosystems and compare the results with morphological analyses. Of 5267 sequences, representing 549 operational taxonomic units (OTU), only 18 OTUs were common to both sites. Representatives included 8 phyla, dominated by arthropods and nematodes. This is the most comprehensive molecular analysis of soil fauna to date, and provides a tool to rapidly assess a missing component of soil biodiversity.

KW - Biodiversity

KW - Soil

KW - DNA sequence

KW - Community structure

KW - Phylogenetic analysis

KW - Morphological analysis

U2 - 10.1016/j.soilbio.2009.02.003

DO - 10.1016/j.soilbio.2009.02.003

M3 - Journal article

VL - 41

SP - 849

EP - 857

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

IS - 4

ER -

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