TY - JOUR

T1 - Molecularly imprinted polymers for cholecystokinin C-terminal pentapeptide

AU - Papaioannou, Emmanouil

AU - Liakopoulou-Kyriakides, Maria

AU - Papi, Rigini M.

AU - Kyriakidis, Dimitrios A.

PY - 2007/12/18

Y1 - 2007/12/18

N2 - Molecularly imprinted polymers (MIPs) have been prepared from methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) as the functional monomer and crosslinker, respectively, for cholecystokinin C terminal pentapeptide (CCK-5), and screened for their rebinding characteristics. They were produced with alterations in the molar ratio of template/monomer/crosslinker and the percentage of net rebinding and the imprinting factor (IF) were used for the evaluation of their imprinting efficacy. The dissociation constant values determined in the case of the polymers with better characteristics, further confirmed the results above.Scanning electron microscopy (SEM) images of MIPs were also obtained in an attempt to correlate their characteristics with polymer’s morphology. Rebinding experiments with CCK-5 and CCK-8 peptides in the case of the polymer that presented higher performance, with net rebinding values 11.9 and 9%, respectively, indicated that this polymer may be a good candidate for the analysis of CCKrelated peptides.

AB - Molecularly imprinted polymers (MIPs) have been prepared from methacrylic acid (MAA) and ethylene glycol dimethacrylate (EGDMA) as the functional monomer and crosslinker, respectively, for cholecystokinin C terminal pentapeptide (CCK-5), and screened for their rebinding characteristics. They were produced with alterations in the molar ratio of template/monomer/crosslinker and the percentage of net rebinding and the imprinting factor (IF) were used for the evaluation of their imprinting efficacy. The dissociation constant values determined in the case of the polymers with better characteristics, further confirmed the results above.Scanning electron microscopy (SEM) images of MIPs were also obtained in an attempt to correlate their characteristics with polymer’s morphology. Rebinding experiments with CCK-5 and CCK-8 peptides in the case of the polymer that presented higher performance, with net rebinding values 11.9 and 9%, respectively, indicated that this polymer may be a good candidate for the analysis of CCKrelated peptides.

KW - cholecystokinin-C terminal pentapeptide

KW - copolymerization

KW - molecular imprinting

KW - morphology

KW - radical polymerization

U2 - 10.1002/macp.200700371

DO - 10.1002/macp.200700371

M3 - Journal article

AN - SCOPUS:37249071276

VL - 208

SP - 2621

EP - 2627

JO - Macromolecular Chemistry and Physics

JF - Macromolecular Chemistry and Physics

SN - 1022-1352

IS - 24

ER -