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Molecularly imprinted polymers for RGD selective recognition and separation

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Molecularly imprinted polymers for RGD selective recognition and separation. / Papaioannou, Emmanouil; Koutsas, Christos; Liakopoulou-Kyriakides, Maria.
In: Amino Acids, Vol. 36, No. 3, 03.2009, p. 563-569.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Papaioannou, E, Koutsas, C & Liakopoulou-Kyriakides, M 2009, 'Molecularly imprinted polymers for RGD selective recognition and separation', Amino Acids, vol. 36, no. 3, pp. 563-569. https://doi.org/10.1007/s00726-008-0118-6

APA

Papaioannou, E., Koutsas, C., & Liakopoulou-Kyriakides, M. (2009). Molecularly imprinted polymers for RGD selective recognition and separation. Amino Acids, 36(3), 563-569. https://doi.org/10.1007/s00726-008-0118-6

Vancouver

Papaioannou E, Koutsas C, Liakopoulou-Kyriakides M. Molecularly imprinted polymers for RGD selective recognition and separation. Amino Acids. 2009 Mar;36(3):563-569. Epub 2008 Jul 1. doi: 10.1007/s00726-008-0118-6

Author

Papaioannou, Emmanouil ; Koutsas, Christos ; Liakopoulou-Kyriakides, Maria. / Molecularly imprinted polymers for RGD selective recognition and separation. In: Amino Acids. 2009 ; Vol. 36, No. 3. pp. 563-569.

Bibtex

@article{aba0ffcc9f8640a6b347d08c8683ca87,
title = "Molecularly imprinted polymers for RGD selective recognition and separation",
abstract = "Molecularly imprinted polymers that could recognize the tripeptide Arg-Gly-Asp have been produced with the use of two functional monomers and three different cross-linkers, respectively. Methacrylic acid and acrylamide were used as functional monomers and the role of the ethylene glycol dimethacrylate, trimethylpropane trimethacrylate and N,N?-methylene-bisacrylamide as crosslinking monomers, was investigated on their recognition capability. The % net rebinding and the imprinting factor values were obtained, giving for the methacrylic acid-trimethylpropane trimethacrylate polymer the highest values 12.3% and 2.44, respectively. In addition, this polymer presented lower dissociation constant (K D) value and the higher B max% of theoretical total binding sites than all the other polymers. Rebinding experiments with Lys-Gly-Asp, an analogue of Arg-Gly-Asp, and other different peptides, such as cholecystokinin C-terminal tri- and pentapeptide and gramicidin, further indicated the selectivity of methacrylic acid-trimethylpropane trimethacrylate copolymer for Arg-Gly-Asp giving specific selectivity factor values 1.27, 1.98, 1.31 and 1.67, respectively.",
keywords = "Arg-Gly-Asp, Copolymerization, Molecular imprinting, Radical polymerization, RGD",
author = "Emmanouil Papaioannou and Christos Koutsas and Maria Liakopoulou-Kyriakides",
year = "2009",
month = mar,
doi = "10.1007/s00726-008-0118-6",
language = "English",
volume = "36",
pages = "563--569",
journal = "Amino Acids",
issn = "0939-4451",
publisher = "Springer Wien",
number = "3",

}

RIS

TY - JOUR

T1 - Molecularly imprinted polymers for RGD selective recognition and separation

AU - Papaioannou, Emmanouil

AU - Koutsas, Christos

AU - Liakopoulou-Kyriakides, Maria

PY - 2009/3

Y1 - 2009/3

N2 - Molecularly imprinted polymers that could recognize the tripeptide Arg-Gly-Asp have been produced with the use of two functional monomers and three different cross-linkers, respectively. Methacrylic acid and acrylamide were used as functional monomers and the role of the ethylene glycol dimethacrylate, trimethylpropane trimethacrylate and N,N?-methylene-bisacrylamide as crosslinking monomers, was investigated on their recognition capability. The % net rebinding and the imprinting factor values were obtained, giving for the methacrylic acid-trimethylpropane trimethacrylate polymer the highest values 12.3% and 2.44, respectively. In addition, this polymer presented lower dissociation constant (K D) value and the higher B max% of theoretical total binding sites than all the other polymers. Rebinding experiments with Lys-Gly-Asp, an analogue of Arg-Gly-Asp, and other different peptides, such as cholecystokinin C-terminal tri- and pentapeptide and gramicidin, further indicated the selectivity of methacrylic acid-trimethylpropane trimethacrylate copolymer for Arg-Gly-Asp giving specific selectivity factor values 1.27, 1.98, 1.31 and 1.67, respectively.

AB - Molecularly imprinted polymers that could recognize the tripeptide Arg-Gly-Asp have been produced with the use of two functional monomers and three different cross-linkers, respectively. Methacrylic acid and acrylamide were used as functional monomers and the role of the ethylene glycol dimethacrylate, trimethylpropane trimethacrylate and N,N?-methylene-bisacrylamide as crosslinking monomers, was investigated on their recognition capability. The % net rebinding and the imprinting factor values were obtained, giving for the methacrylic acid-trimethylpropane trimethacrylate polymer the highest values 12.3% and 2.44, respectively. In addition, this polymer presented lower dissociation constant (K D) value and the higher B max% of theoretical total binding sites than all the other polymers. Rebinding experiments with Lys-Gly-Asp, an analogue of Arg-Gly-Asp, and other different peptides, such as cholecystokinin C-terminal tri- and pentapeptide and gramicidin, further indicated the selectivity of methacrylic acid-trimethylpropane trimethacrylate copolymer for Arg-Gly-Asp giving specific selectivity factor values 1.27, 1.98, 1.31 and 1.67, respectively.

KW - Arg-Gly-Asp

KW - Copolymerization

KW - Molecular imprinting

KW - Radical polymerization

KW - RGD

U2 - 10.1007/s00726-008-0118-6

DO - 10.1007/s00726-008-0118-6

M3 - Journal article

C2 - 18592344

AN - SCOPUS:67349200922

VL - 36

SP - 563

EP - 569

JO - Amino Acids

JF - Amino Acids

SN - 0939-4451

IS - 3

ER -