Chemical defense in larvae of the plant bug Hotea gambiae has been investigated. Results of analyses (GC, GC-MS) on the secretions from the three dorsally situated larval abdominal defense (scent) glands are reported. The secretion from the first abdominal gland consists of a mixture of C₁₀ and C₁₅ isoprenoids: (C₁₀) α-pinene, β-pinene, limonene, β-phellandrene; (C₁₅) β-caryophyllene, caryophyllene oxide, α-humulene, and (the major component) humulene epoxide II. The secretions from the second and third abdominal glands are similar mixtures consisting of (E)-2-decenal, (E)-4-oxohex-2-enal, and n-tridecane together with lesser amounts of (E)-2-hexenal, n-dodecane, and other materials. Isoprenoid defense is now known from four species of plant bugs (Heteroptera) associated with Malvaceae.