Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - N-acetyl-cysteine causes a late re-specification of the anteroposterior axis in the Xenopus embryo
AU - Gatherer, Derek
AU - Woodland, Hugh R.
PY - 1996/4
Y1 - 1996/4
N2 - N-acetyl cysteine is an agent which has been shown to interrupt signal transduction processes linking a wide range of stimuli to the activation of NF-KB in mammalian cells. We have investigated its effect on the early development of Xenopus embryos by injecting it into blastulae, using concentrations comparable to those effective on cultured cells. High concentrations at the late blastula or early gastrula stage suppress posterior and enhance anterior development, yielding embryos with enlarged cement glands and otherwise consisting of little except head in extreme cases. Reducing the amount of N-acetyl cysteine injected leads to progressively more posterior structures developing. Injection into one- or two-cell embryos gives similar phenotypes, but of reduced severity and the cement gland is not so enlarged, Explants of animal cap cells taken several hours after injection develop to give large amounts of cement gland material. We have examined the expression of a number of genes in the anteriorised embryos. Posterior markers and Xsna are reduced. Noggin and Goosecoid mRNA are up-regulated through the gastrula and persist at these levels until at least the late neurula stage, whereas in controls Noggin is much lower and Goosecoid is absent at these stages. The most anteriorised phenotype may be a consequence of this changed expression. (C) 1996 Wiley-Liss, Inc.
AB - N-acetyl cysteine is an agent which has been shown to interrupt signal transduction processes linking a wide range of stimuli to the activation of NF-KB in mammalian cells. We have investigated its effect on the early development of Xenopus embryos by injecting it into blastulae, using concentrations comparable to those effective on cultured cells. High concentrations at the late blastula or early gastrula stage suppress posterior and enhance anterior development, yielding embryos with enlarged cement glands and otherwise consisting of little except head in extreme cases. Reducing the amount of N-acetyl cysteine injected leads to progressively more posterior structures developing. Injection into one- or two-cell embryos gives similar phenotypes, but of reduced severity and the cement gland is not so enlarged, Explants of animal cap cells taken several hours after injection develop to give large amounts of cement gland material. We have examined the expression of a number of genes in the anteriorised embryos. Posterior markers and Xsna are reduced. Noggin and Goosecoid mRNA are up-regulated through the gastrula and persist at these levels until at least the late neurula stage, whereas in controls Noggin is much lower and Goosecoid is absent at these stages. The most anteriorised phenotype may be a consequence of this changed expression. (C) 1996 Wiley-Liss, Inc.
KW - Xenopus development
KW - cement gland
KW - positional information
KW - axis formation
KW - macrocephaly
KW - N-acetyl cysteine
KW - NF-kappa B
KW - CENTRAL-NERVOUS-SYSTEM
KW - HOMEOBOX GENE XHOX3
KW - ANIMAL CAP CELLS
KW - FROG EMBRYOS
KW - KAPPA-B
KW - SPEMANN ORGANIZER
KW - MESODERM INDUCTION
KW - NEURAL INDUCTION
KW - LAEVIS EMBRYOS
KW - CEMENT-GLAND
U2 - 10.1002/(SICI)1097-0177(199604)205:4<395::AID-AJA4>3.0.CO;2-D
DO - 10.1002/(SICI)1097-0177(199604)205:4<395::AID-AJA4>3.0.CO;2-D
M3 - Journal article
VL - 205
SP - 395
EP - 409
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 4
ER -