Final published version
Licence: CC BY: Creative Commons Attribution 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Nasopharyngeal metagenomic deep sequencing data, Lancaster, UK, 2014-2015
AU - Atkinson, Kate V.
AU - Bishop, Lisa A.
AU - Rhodes, Glenn
AU - Salez, Nicolas
AU - McEwan, Neil R.
AU - Hegarty, Matthew J.
AU - Robey, Julie
AU - Harding, Nicola
AU - Wetherell, Simon
AU - Lauder, Robert M.
AU - Pickup, Roger W.
AU - Wilkinson, Mark
AU - Gatherer, Derek
PY - 2017/10/24
Y1 - 2017/10/24
N2 - Nasopharyngeal swabs were taken from volunteers attending a general medical practice and a general hospital in Lancaster, UK, and at Lancaster University, in the winter of 2014-2015. 51 swabs were selected based on high RNA yield and allocated to deep sequencing pools as follows: patients with chronic obstructive pulmonary disease; asthmatics; adults with no respiratory symptoms; adults with feverish respiratory symptoms; adults with respiratory symptoms and presence of antibodies against influenza C; paediatric patients with respiratory symptoms (2 pools); adults with influenza C infection (2 pools), giving a total of 9 pools. Illumina sequencing was performed, with data yields per pool in the range of 345.6 megabases to 14 gigabases after removal of reads aligning to the human genome. The data were deposited in the Sequence Read Archive at NCBI, and constitute a resource for study of the viral, bacterial and fungal metagenome of the human nasopharynx in healthy and diseased states and comparison with other metagenomic studies on the human respiratory tract.
AB - Nasopharyngeal swabs were taken from volunteers attending a general medical practice and a general hospital in Lancaster, UK, and at Lancaster University, in the winter of 2014-2015. 51 swabs were selected based on high RNA yield and allocated to deep sequencing pools as follows: patients with chronic obstructive pulmonary disease; asthmatics; adults with no respiratory symptoms; adults with feverish respiratory symptoms; adults with respiratory symptoms and presence of antibodies against influenza C; paediatric patients with respiratory symptoms (2 pools); adults with influenza C infection (2 pools), giving a total of 9 pools. Illumina sequencing was performed, with data yields per pool in the range of 345.6 megabases to 14 gigabases after removal of reads aligning to the human genome. The data were deposited in the Sequence Read Archive at NCBI, and constitute a resource for study of the viral, bacterial and fungal metagenome of the human nasopharynx in healthy and diseased states and comparison with other metagenomic studies on the human respiratory tract.
KW - nasopharyngeal metagenomes
KW - metagenomics
KW - respiratory metagenomics
KW - deep sequencing
KW - viruses
KW - bioinformatics
U2 - 10.1038/sdata.2017.161
DO - 10.1038/sdata.2017.161
M3 - Journal article
C2 - 29064471
VL - 4
JO - Scientific Data
JF - Scientific Data
SN - 2052-4463
M1 - 170161
ER -