Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Nickel elicits a fast antioxidant response in Coffea arabica cells.
AU - Gomes-Junior, R. A.
AU - Moldes, C. A.
AU - Gratão, P. L.
AU - Mazzafera, P.
AU - Lea, P. J.
AU - Azevedo, R. A.
PY - 2006
Y1 - 2006
N2 - The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to nickel (Ni) were investigated. Ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of NiCl2 concentration in the medium. At 0.05 mM NiCl2 growth was stimulated, but at 0.5 mM NiCl2, the growth rate was reduced. An indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mM NiCl2. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GOPX; EC 1.11.1.7) and superoxide dismutase (SOD; EC 1.15.1.1) activities were increased, particularly at earlier NiCl2 exposure times and the activities were higher at 0.5 mM NiCl2 for most of exposure times tested. Non-denaturing PAGE revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differentially affected by NiCl2 treatment and one GR isoenzyme was increased by NiCl2. NiCl2 at 0.05 mM did not induce lipid peroxidation and the main response appeared to be via the induction of SOD, CAT, GOPX and APX activities for the removal of the reactive oxygen species and through the induction of GR to ensure the availability of reduced glutathione.
AB - The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to nickel (Ni) were investigated. Ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of NiCl2 concentration in the medium. At 0.05 mM NiCl2 growth was stimulated, but at 0.5 mM NiCl2, the growth rate was reduced. An indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mM NiCl2. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GOPX; EC 1.11.1.7) and superoxide dismutase (SOD; EC 1.15.1.1) activities were increased, particularly at earlier NiCl2 exposure times and the activities were higher at 0.5 mM NiCl2 for most of exposure times tested. Non-denaturing PAGE revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differentially affected by NiCl2 treatment and one GR isoenzyme was increased by NiCl2. NiCl2 at 0.05 mM did not induce lipid peroxidation and the main response appeared to be via the induction of SOD, CAT, GOPX and APX activities for the removal of the reactive oxygen species and through the induction of GR to ensure the availability of reduced glutathione.
KW - Coffea arabica
KW - Oxidative stress
KW - Lipid peroxidation
KW - Nickel
KW - Catalase
KW - Glutathione reductase
KW - Superoxide dismutase
U2 - 10.1016/j.plaphy.2006.06.002
DO - 10.1016/j.plaphy.2006.06.002
M3 - Journal article
VL - 44
SP - 420
EP - 429
JO - Plant Physiology and Biochemistry
JF - Plant Physiology and Biochemistry
SN - 0981-9428
IS - 5-6
ER -