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Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks

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Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. / Johnson, D; Leake, JR; Read, DJ.
In: New Phytologist, Vol. 152, No. 3, 31.12.2001, p. 555-562.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Johnson, D, Leake, JR & Read, DJ 2001, 'Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks', New Phytologist, vol. 152, no. 3, pp. 555-562. https://doi.org/10.1046/j.0028-646X.2001.00273.x

APA

Johnson, D., Leake, JR., & Read, DJ. (2001). Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. New Phytologist, 152(3), 555-562. https://doi.org/10.1046/j.0028-646X.2001.00273.x

Vancouver

Johnson D, Leake JR, Read DJ. Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. New Phytologist. 2001 Dec 31;152(3):555-562. doi: 10.1046/j.0028-646X.2001.00273.x

Author

Johnson, D ; Leake, JR ; Read, DJ. / Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks. In: New Phytologist. 2001 ; Vol. 152, No. 3. pp. 555-562.

Bibtex

@article{2358eb71b8724dc990e43570ede8ddc6,
title = "Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks",
abstract = "A novel in-growth core system, enabling functional studies of natural communities of arbuscular mycorrhizal (AM) mycelia in soil is described and tested.The cores have windows covered with nylon mesh of 35 µm pore size that prevent in-growth of roots but permit penetration of AM hyphae. They were inserted into grassland turf and contained either sterilized sand and a {\textquoteleft}bait{\textquoteright} seedling of Trifolium repens or nonsterile natural soil without bait plants. The impacts of hyphal severance, achieved by periodic rotation of some of the cores, upon AM colonization of bait plants (experiment 1) and transfer of 33P from soil to plants outside the cores (experiment 2) were examined.Severance of AM hyphae reduced both AM colonization of bait plants and their shoot P concentrations. The shoot 33P concentrations of plants with mycelial access to 33PO4-labelled cores were 10-fold greater than those which had no mycelial access.It is concluded that this novel approach enables the functioning of mycorrhizal mycelial networks to be evaluated under conditions closely simulating those occurring in nature.",
keywords = "AM mycorrhizal colonization, Trifolium repens (white clover), mycelial networks, phosphorus uptake, P-33, hyphal exclusion",
author = "D Johnson and JR Leake and DJ Read",
year = "2001",
month = dec,
day = "31",
doi = "10.1046/j.0028-646X.2001.00273.x",
language = "English",
volume = "152",
pages = "555--562",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "3",

}

RIS

TY - JOUR

T1 - Novel in-growth core system enables functional studies of grassland mycorrhizal mycelial networks

AU - Johnson, D

AU - Leake, JR

AU - Read, DJ

PY - 2001/12/31

Y1 - 2001/12/31

N2 - A novel in-growth core system, enabling functional studies of natural communities of arbuscular mycorrhizal (AM) mycelia in soil is described and tested.The cores have windows covered with nylon mesh of 35 µm pore size that prevent in-growth of roots but permit penetration of AM hyphae. They were inserted into grassland turf and contained either sterilized sand and a ‘bait’ seedling of Trifolium repens or nonsterile natural soil without bait plants. The impacts of hyphal severance, achieved by periodic rotation of some of the cores, upon AM colonization of bait plants (experiment 1) and transfer of 33P from soil to plants outside the cores (experiment 2) were examined.Severance of AM hyphae reduced both AM colonization of bait plants and their shoot P concentrations. The shoot 33P concentrations of plants with mycelial access to 33PO4-labelled cores were 10-fold greater than those which had no mycelial access.It is concluded that this novel approach enables the functioning of mycorrhizal mycelial networks to be evaluated under conditions closely simulating those occurring in nature.

AB - A novel in-growth core system, enabling functional studies of natural communities of arbuscular mycorrhizal (AM) mycelia in soil is described and tested.The cores have windows covered with nylon mesh of 35 µm pore size that prevent in-growth of roots but permit penetration of AM hyphae. They were inserted into grassland turf and contained either sterilized sand and a ‘bait’ seedling of Trifolium repens or nonsterile natural soil without bait plants. The impacts of hyphal severance, achieved by periodic rotation of some of the cores, upon AM colonization of bait plants (experiment 1) and transfer of 33P from soil to plants outside the cores (experiment 2) were examined.Severance of AM hyphae reduced both AM colonization of bait plants and their shoot P concentrations. The shoot 33P concentrations of plants with mycelial access to 33PO4-labelled cores were 10-fold greater than those which had no mycelial access.It is concluded that this novel approach enables the functioning of mycorrhizal mycelial networks to be evaluated under conditions closely simulating those occurring in nature.

KW - AM mycorrhizal colonization

KW - Trifolium repens (white clover)

KW - mycelial networks

KW - phosphorus uptake

KW - P-33

KW - hyphal exclusion

U2 - 10.1046/j.0028-646X.2001.00273.x

DO - 10.1046/j.0028-646X.2001.00273.x

M3 - Journal article

VL - 152

SP - 555

EP - 562

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 3

ER -