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Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device

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Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device. / Baldock, S. J.; Fielden, P. R.; Goddard, N. J.; Kretschmer, H. R.; Prest, J. E.; Brown, B. J. T.

In: Journal of Chromatography A, Vol. 1042, No. 1-2, 09.07.2004, p. 181-188.

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Baldock, S. J. ; Fielden, P. R. ; Goddard, N. J. ; Kretschmer, H. R. ; Prest, J. E. ; Brown, B. J. T. / Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device. In: Journal of Chromatography A. 2004 ; Vol. 1042, No. 1-2. pp. 181-188.

Bibtex

@article{bf2f5183a33a463ab7299c5081eef713,
title = "Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device",
abstract = "A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.",
keywords = "Isotachphoresis, Miniaturization, Injection methods, Instrumentation, Chip technology",
author = "Baldock, {S. J.} and Fielden, {P. R.} and Goddard, {N. J.} and Kretschmer, {H. R.} and Prest, {J. E.} and Brown, {B. J. T.}",
year = "2004",
month = jul,
day = "9",
doi = "10.1016/j.chroma.2004.05.062",
language = "English",
volume = "1042",
pages = "181--188",
journal = "Journal of Chromatography A",
issn = "0021-9673",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device

AU - Baldock, S. J.

AU - Fielden, P. R.

AU - Goddard, N. J.

AU - Kretschmer, H. R.

AU - Prest, J. E.

AU - Brown, B. J. T.

PY - 2004/7/9

Y1 - 2004/7/9

N2 - A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.

AB - A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.

KW - Isotachphoresis

KW - Miniaturization

KW - Injection methods

KW - Instrumentation

KW - Chip technology

U2 - 10.1016/j.chroma.2004.05.062

DO - 10.1016/j.chroma.2004.05.062

M3 - Journal article

VL - 1042

SP - 181

EP - 188

JO - Journal of Chromatography A

JF - Journal of Chromatography A

SN - 0021-9673

IS - 1-2

ER -