Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device. / Baldock, S. J.; Fielden, P. R.; Goddard, N. J. et al.
In: Journal of Chromatography A, Vol. 1042, No. 1-2, 09.07.2004, p. 181-188.Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Novel variable volume injector for performing sample introduction in a miniaturised isotachophoresis device
AU - Baldock, S. J.
AU - Fielden, P. R.
AU - Goddard, N. J.
AU - Kretschmer, H. R.
AU - Prest, J. E.
AU - Brown, B. J. T.
PY - 2004/7/9
Y1 - 2004/7/9
N2 - A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.
AB - A microdevice design furnished with a novel sample injector, capable of delivering variable volume samples, for miniaturised isotachophoretic separations is presented. Micromachining by direct milling was used to realise two flow channel network designs on poly(methyl methacrylate) chips. Both designs comprised a wide bore sample channel interfaced, via a short connection channel, to a narrow bore separation channel. Superior injection performance was observed with a connection channel angled at 45° to the separation channel compared to a device using a channel angled at 90°. Automated delivery of electrolytes to the microdevice was demonstrated with both hydrostatic pumping and syringe pumps; both gave reproducible sample injection. A range of different sampling strategies were investigated. Isotachophoretic separations of model analytes (metal ions and an anionic dye) demonstrated the potential of the device. Separations of ten metal cations were achieved in under 475 s.
KW - Isotachphoresis
KW - Miniaturization
KW - Injection methods
KW - Instrumentation
KW - Chip technology
U2 - 10.1016/j.chroma.2004.05.062
DO - 10.1016/j.chroma.2004.05.062
M3 - Journal article
VL - 1042
SP - 181
EP - 188
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 1-2
ER -