Rights statement: This is an Accepted Manuscript of an article published by Taylor & Francis in Analytical Letters on 04/9/2019, available online: https://www.tandfonline.com/doi/full/10.1080/00032719.2019.1658199
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Observation of nutrient uptake at the adaxial surface of leaves of tomato (Solanum lycopersicum) using Raman spectroscopy
AU - Butler, Holly
AU - Martin, Frank
AU - Roberts, Mike
AU - Adams, Steven
AU - McAinsh, Martin
N1 - This is an Accepted Manuscript of an article published by Taylor & Francis in Analytical Letters on 04/9/2019, available online: https://www.tandfonline.com/doi/full/10.1080/00032719.2019.1658199
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Foliar application of nutrient fertilizers is standard practice in agricul- tural environments, and has been shown to increase crop yield and quality more efficiently and economically than soil-based fertilizers. The adsorption of macro- and micro-nutrients through the upper epidermis of leaves is largely species dependent; reliant upon pene- tration through the cuticle and stomata, and also upon the plant’s ability to translocate the nutrient. Herein we describe a method to observe nitrate (NO3) uptake at the adaxial leaf surface to deter- mine the efficacy of foliar fertilizers. We use Raman microspectro- scopy as a sensitive approach to monitor NO3 associated vibrational modes, complemented by ion probe measurements and measurements of leaf nutrient status using flame atomic absorption spectroscopy. The results show that NO3 uptake can be observed down to concentrations as low as 15 mM using Raman microspectro- scopy over a defined surface area, and that the rate of uptake can also be quantified using this approach. These observations could also infer information regarding the transport of other ions present in nitrate salts, such as calcium (Ca), via the indirect monitoring of NO3- specific bands. We believe that Raman microspectroscopy pro- vides a novel method for monitoring nutrient movement throughout plant tissue, and provides a potential tool for nutrient screening.
AB - Foliar application of nutrient fertilizers is standard practice in agricul- tural environments, and has been shown to increase crop yield and quality more efficiently and economically than soil-based fertilizers. The adsorption of macro- and micro-nutrients through the upper epidermis of leaves is largely species dependent; reliant upon pene- tration through the cuticle and stomata, and also upon the plant’s ability to translocate the nutrient. Herein we describe a method to observe nitrate (NO3) uptake at the adaxial leaf surface to deter- mine the efficacy of foliar fertilizers. We use Raman microspectro- scopy as a sensitive approach to monitor NO3 associated vibrational modes, complemented by ion probe measurements and measurements of leaf nutrient status using flame atomic absorption spectroscopy. The results show that NO3 uptake can be observed down to concentrations as low as 15 mM using Raman microspectro- scopy over a defined surface area, and that the rate of uptake can also be quantified using this approach. These observations could also infer information regarding the transport of other ions present in nitrate salts, such as calcium (Ca), via the indirect monitoring of NO3- specific bands. We believe that Raman microspectroscopy pro- vides a novel method for monitoring nutrient movement throughout plant tissue, and provides a potential tool for nutrient screening.
KW - Crop screening
KW - food security
KW - nutrient uptake
KW - Raman spectroscopy
U2 - 10.1080/00032719.2019.1658199
DO - 10.1080/00032719.2019.1658199
M3 - Journal article
VL - 53
SP - 536
EP - 562
JO - Analytical Letters
JF - Analytical Letters
SN - 0003-2719
IS - 4
ER -