Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Ocurrence of the antibiotic producing bacterium Burkholderia sp in colonies of the leaf-cutting ant Atta sexdens rubropilosa
AU - Santos, A V
AU - Dillon, R J
AU - Dillon, V M
AU - Reynolds, S E
AU - Samuels, R I
PY - 2004/10/15
Y1 - 2004/10/15
N2 - Fungus garden material from recently established Atta sexdens rubropilosa colonies (6-12 months old) was sampled to detect antibiotic producing microorganisms that inhibited the growth of pathogens of insects and of the fungus gardens but did not affect their mutualistic fungus. A bacterium with activity against the entomopathogenic fungus Beauveria bassiana was isolated from 56% of the gardens tested (n = 57) and identified from its biochemical profile and from 16S and 23S ribosomal DNA sequences as a member of the genus Burkholderia. The ant-associated Burkholderia isolates secreted a potent, anti-fungal agent that inhibited germination of conidia of the entomopathogenic fungi B. bassiana, Metarhizium anisopliae, of the saprophytic Verticillium lecanii, and also of a specialist fungus garden Escovopsis weberi. Growth of the ant's mutualist fungus was unaffected.
AB - Fungus garden material from recently established Atta sexdens rubropilosa colonies (6-12 months old) was sampled to detect antibiotic producing microorganisms that inhibited the growth of pathogens of insects and of the fungus gardens but did not affect their mutualistic fungus. A bacterium with activity against the entomopathogenic fungus Beauveria bassiana was isolated from 56% of the gardens tested (n = 57) and identified from its biochemical profile and from 16S and 23S ribosomal DNA sequences as a member of the genus Burkholderia. The ant-associated Burkholderia isolates secreted a potent, anti-fungal agent that inhibited germination of conidia of the entomopathogenic fungi B. bassiana, Metarhizium anisopliae, of the saprophytic Verticillium lecanii, and also of a specialist fungus garden Escovopsis weberi. Growth of the ant's mutualist fungus was unaffected.
KW - antibiotic
KW - pathogenic microorganism
KW - biological control
KW - Burkholderia
KW - entomopathogenic fungi
KW - leaf-cutting ant
KW - PSEUDOMONAS-CEPACIA
KW - BIOLOGICAL-CONTROL
KW - DNA FRAGMENTS
KW - RIBOSOMAL-RNA
KW - DIVERSITY
KW - RECOVERY
KW - IDENTIFICATION
KW - SYMBIOSIS
KW - COMPLEX
KW - MEDIA
U2 - 10.1016/j.femsle.2004.09.005
DO - 10.1016/j.femsle.2004.09.005
M3 - Journal article
VL - 239
SP - 319
EP - 323
JO - FEMS Microbiology Letters
JF - FEMS Microbiology Letters
SN - 0378-1097
IS - 2
ER -