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Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing.

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Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing. / Rojas Martinez, Federico; Silvester, Eleanor ; Young, Julie et al.
In: Cell, Vol. 176, No. 1-2, 10.01.2019, p. 306-317.e16.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Rojas Martinez, F, Silvester, E, Young, J, Milne, R, Tettey, MD, Houston, DR, Walkinshaw, MD, Perez-Pi, I, Auer, M, Denton, H, Smith, TK, Thompson, J & Matthews, KR 2019, 'Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing.', Cell, vol. 176, no. 1-2, pp. 306-317.e16. https://doi.org/10.1016/j.cell.2018.10.041

APA

Rojas Martinez, F., Silvester, E., Young, J., Milne, R., Tettey, M. D., Houston, D. R., Walkinshaw, M. D., Perez-Pi, I., Auer, M., Denton, H., Smith, T. K., Thompson, J., & Matthews, K. R. (2019). Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing. Cell, 176(1-2), 306-317.e16. https://doi.org/10.1016/j.cell.2018.10.041

Vancouver

Rojas Martinez F, Silvester E, Young J, Milne R, Tettey MD, Houston DR et al. Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing. Cell. 2019 Jan 10;176(1-2):306-317.e16. doi: 10.1016/j.cell.2018.10.041

Author

Rojas Martinez, Federico ; Silvester, Eleanor ; Young, Julie et al. / Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing. In: Cell. 2019 ; Vol. 176, No. 1-2. pp. 306-317.e16.

Bibtex

@article{7652ffcb07074348a272b4cab4ac3214,
title = "Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing.",
abstract = "Trypanosome parasites control their virulence and spread by using quorum sensing (QS) to generate transmissible {"}stumpy forms{"} in their host bloodstream. However, the QS signal {"}stumpy induction factor{"} (SIF) and its reception mechanism are unknown. Although trypanosomes lack G protein-coupled receptor signaling, we have identified a surface GPR89-family protein that regulates stumpy formation. TbGPR89 is expressed on bloodstream {"}slender form{"} trypanosomes, which receive the SIF signal, and when ectopically expressed, TbGPR89 drives stumpy formation in a SIF-pathway-dependent process. Structural modeling of TbGPR89 predicts unexpected similarity to oligopeptide transporters (POT), and when expressed in bacteria, TbGPR89 transports oligopeptides. Conversely, expression of an E. coli POT in trypanosomes drives parasite differentiation, and oligopeptides promote stumpy formation in vitro. Furthermore, the expression of secreted trypanosome oligopeptidases generates a paracrine signal that accelerates stumpy formation in vivo. Peptidase-generated oligopeptide QS signals being received through TbGPR89 provides a mechanism for both trypanosome SIF production and reception.",
keywords = "Trypanosome brucei, parasite, quorum sensing, stumpy induction factor, differentiation, GPR89, oligopeptide, sleeping sickness",
author = "{Rojas Martinez}, Federico and Eleanor Silvester and Julie Young and Rachel Milne and Tettey, {Mabel Deladem} and Houston, {Douglas R.} and Walkinshaw, {Malcolm D.} and Irene Perez-Pi and Manfred Auer and Helen Denton and Smith, {Terry K} and Joanne Thompson and Matthews, {Keith R.}",
year = "2019",
month = jan,
day = "10",
doi = "10.1016/j.cell.2018.10.041",
language = "English",
volume = "176",
pages = "306--317.e16",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "1-2",

}

RIS

TY - JOUR

T1 - Oligopeptide Signaling through TbGPR89 Drives Trypanosome Quorum Sensing.

AU - Rojas Martinez, Federico

AU - Silvester, Eleanor

AU - Young, Julie

AU - Milne, Rachel

AU - Tettey, Mabel Deladem

AU - Houston, Douglas R.

AU - Walkinshaw, Malcolm D.

AU - Perez-Pi, Irene

AU - Auer, Manfred

AU - Denton, Helen

AU - Smith, Terry K

AU - Thompson, Joanne

AU - Matthews, Keith R.

PY - 2019/1/10

Y1 - 2019/1/10

N2 - Trypanosome parasites control their virulence and spread by using quorum sensing (QS) to generate transmissible "stumpy forms" in their host bloodstream. However, the QS signal "stumpy induction factor" (SIF) and its reception mechanism are unknown. Although trypanosomes lack G protein-coupled receptor signaling, we have identified a surface GPR89-family protein that regulates stumpy formation. TbGPR89 is expressed on bloodstream "slender form" trypanosomes, which receive the SIF signal, and when ectopically expressed, TbGPR89 drives stumpy formation in a SIF-pathway-dependent process. Structural modeling of TbGPR89 predicts unexpected similarity to oligopeptide transporters (POT), and when expressed in bacteria, TbGPR89 transports oligopeptides. Conversely, expression of an E. coli POT in trypanosomes drives parasite differentiation, and oligopeptides promote stumpy formation in vitro. Furthermore, the expression of secreted trypanosome oligopeptidases generates a paracrine signal that accelerates stumpy formation in vivo. Peptidase-generated oligopeptide QS signals being received through TbGPR89 provides a mechanism for both trypanosome SIF production and reception.

AB - Trypanosome parasites control their virulence and spread by using quorum sensing (QS) to generate transmissible "stumpy forms" in their host bloodstream. However, the QS signal "stumpy induction factor" (SIF) and its reception mechanism are unknown. Although trypanosomes lack G protein-coupled receptor signaling, we have identified a surface GPR89-family protein that regulates stumpy formation. TbGPR89 is expressed on bloodstream "slender form" trypanosomes, which receive the SIF signal, and when ectopically expressed, TbGPR89 drives stumpy formation in a SIF-pathway-dependent process. Structural modeling of TbGPR89 predicts unexpected similarity to oligopeptide transporters (POT), and when expressed in bacteria, TbGPR89 transports oligopeptides. Conversely, expression of an E. coli POT in trypanosomes drives parasite differentiation, and oligopeptides promote stumpy formation in vitro. Furthermore, the expression of secreted trypanosome oligopeptidases generates a paracrine signal that accelerates stumpy formation in vivo. Peptidase-generated oligopeptide QS signals being received through TbGPR89 provides a mechanism for both trypanosome SIF production and reception.

KW - Trypanosome brucei

KW - parasite

KW - quorum sensing

KW - stumpy induction factor

KW - differentiation

KW - GPR89

KW - oligopeptide

KW - sleeping sickness

U2 - 10.1016/j.cell.2018.10.041

DO - 10.1016/j.cell.2018.10.041

M3 - Journal article

C2 - 30503212

VL - 176

SP - 306-317.e16

JO - Cell

JF - Cell

SN - 0092-8674

IS - 1-2

ER -