Final published version
Licence: CC BY: Creative Commons Attribution 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - On cold atmospheric-pressure plasma jet induced DNA damage in cells
AU - Gaur, N.
AU - Kurita, H.
AU - Oh, J.-S.
AU - Miyachika, S.
AU - Ito, M.
AU - Mizuno, A.
AU - Cowin, A.J.
AU - Allinson, S.
AU - Short, R.D.
AU - Szili, E.J.
PY - 2021/1/30
Y1 - 2021/1/30
N2 - To investigate the potential role of the hydroxyl radical (•OH) in cold atmospheric plasma (CAP) jet treatment, two fluorescence-based methodologies are utilised to measure DNA strand breaks. The first comprises a model system of a double-stranded DNA oligomer, where the respective strand ends are tagged with fluorophore and quencher molecules; and the second, a cell culture system reporting DNA strand breaks using the γ-H2AX assay. During the various CAP jet treatments, optical emission spectroscopy is used to detect the •OH in the gas phase and electron spin resonance is used to detect the •OH in solution. The CAP jet production of the •OH is shown to correlate to CAP jet induced DNA damage both with the DNA model and in biological cells. Results indicate that the CAP jet induces a higher degree of DNA damage when the CAP plume is in contact with the target solution. The potential of a 'plasma screen' based upon a hydrogel film, as a method to remove the DNA-damaging •OH species from reaching skin cells, is shown to significantly reduce DNA damage whilst facilitating the delivery of hydrogen peroxide. These findings could aid in the development of CAP jet-based applications where DNA damage is the objective (e.g. in cancer treatment) and others where it is to be avoided, e.g. in open-wound treatment and dermatology. © 2020 The Author(s). Published by IOP Publishing Ltd.
AB - To investigate the potential role of the hydroxyl radical (•OH) in cold atmospheric plasma (CAP) jet treatment, two fluorescence-based methodologies are utilised to measure DNA strand breaks. The first comprises a model system of a double-stranded DNA oligomer, where the respective strand ends are tagged with fluorophore and quencher molecules; and the second, a cell culture system reporting DNA strand breaks using the γ-H2AX assay. During the various CAP jet treatments, optical emission spectroscopy is used to detect the •OH in the gas phase and electron spin resonance is used to detect the •OH in solution. The CAP jet production of the •OH is shown to correlate to CAP jet induced DNA damage both with the DNA model and in biological cells. Results indicate that the CAP jet induces a higher degree of DNA damage when the CAP plume is in contact with the target solution. The potential of a 'plasma screen' based upon a hydrogel film, as a method to remove the DNA-damaging •OH species from reaching skin cells, is shown to significantly reduce DNA damage whilst facilitating the delivery of hydrogen peroxide. These findings could aid in the development of CAP jet-based applications where DNA damage is the objective (e.g. in cancer treatment) and others where it is to be avoided, e.g. in open-wound treatment and dermatology. © 2020 The Author(s). Published by IOP Publishing Ltd.
KW - DNA damage
KW - hydroxyl radicals (OH)
KW - plasma jet
KW - plasma medicine
KW - reactive oxygen species (ROS)
KW - γ H2AX, HaCaTs
KW - Atmospheric pressure
KW - Cell culture
KW - Cells
KW - Electron spin resonance spectroscopy
KW - Magnetic moments
KW - Optical emission spectroscopy
KW - Plasma jets
KW - Atmospheric pressure plasma jets
KW - Biological cells
KW - Cold atmospheric plasmas
KW - DNA strand break
KW - Double stranded DNA
KW - Hydroxyl radicals
KW - Jet productions
KW - Target solution
KW - DNA
U2 - 10.1088/1361-6463/abb8ab
DO - 10.1088/1361-6463/abb8ab
M3 - Journal article
VL - 54
JO - Journal of Physics D: Applied Physics
JF - Journal of Physics D: Applied Physics
SN - 0022-3727
IS - 3
M1 - 035203
ER -