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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - On Plasma Activated Acetyl Donors
T2 - Comparing the Antibacterial Efficacy of Tetraacetylethylenediamine and Pentaacetate Glucose
AU - Szili, Endre J.
AU - Patenall, Bethany L.
AU - Fellows, Adrian
AU - Mistry, Dharmit
AU - Jenkins, A. Toby A.
AU - Short, Robert D.
AU - Ghimire, Bhagirath
A2 - Starikovskiy, Andrey
PY - 2022/10/13
Y1 - 2022/10/13
N2 - The study compares how acetyl donor molecules tetraacetylethylenediamine (TAED) and pentaacetate glucose (PAG) improve the antibacterial efficacy of solutions activated with a low-temperature atmospheric-pressure argon plasma jet. Plasma activation of solubilised TAED and PAG produce solutions with different chemical compositions and oxidative potentials. Both acetyl donor molecules enhance the hydrogen peroxide (H2O2) concentration in solution with TAED being more effective compared to PAG. However, PAG is more effective at forming peracetic acid (PAA) from reaction of its acetyl donor groups with plasma generated H2O2. The enhanced oxidative potential of plasma activated TAED and PAG solutions were shown to significantly improve bactericidal activity against common wound pathogens Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus compared to plasma activated water produced without acetyl donors. Furthermore, the oxidative capacity of plasma activated PAG was least affected by the bacterial oxidative defence enzyme catalase, attributed to the high concentration of PAA produced in this formulation. Overall, the above data show that acetyl donors may help improve next generation of antimicrobial formulations produced by plasma, which might help combat increasing problems of antimicrobial resistance.
AB - The study compares how acetyl donor molecules tetraacetylethylenediamine (TAED) and pentaacetate glucose (PAG) improve the antibacterial efficacy of solutions activated with a low-temperature atmospheric-pressure argon plasma jet. Plasma activation of solubilised TAED and PAG produce solutions with different chemical compositions and oxidative potentials. Both acetyl donor molecules enhance the hydrogen peroxide (H2O2) concentration in solution with TAED being more effective compared to PAG. However, PAG is more effective at forming peracetic acid (PAA) from reaction of its acetyl donor groups with plasma generated H2O2. The enhanced oxidative potential of plasma activated TAED and PAG solutions were shown to significantly improve bactericidal activity against common wound pathogens Gram-negative Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus compared to plasma activated water produced without acetyl donors. Furthermore, the oxidative capacity of plasma activated PAG was least affected by the bacterial oxidative defence enzyme catalase, attributed to the high concentration of PAA produced in this formulation. Overall, the above data show that acetyl donors may help improve next generation of antimicrobial formulations produced by plasma, which might help combat increasing problems of antimicrobial resistance.
KW - Article
KW - low-temperature atmospheric-pressure argon plasma jet
KW - tetraacetylethylenediamine
KW - pentaacetate glucose
KW - hydrogen peroxide
KW - peracetic acid
KW - antibacterial
KW - antimicrobial resistance
U2 - 10.3390/plasma5040031
DO - 10.3390/plasma5040031
M3 - Journal article
VL - 5
SP - 423
EP - 435
JO - PLASMA
JF - PLASMA
SN - 2571-6182
IS - 4
ER -