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Ontogeny and distribution of Fcγ receptors in the human placenta: Transport or immune surveillance?

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Ontogeny and distribution of Fcγ receptors in the human placenta: Transport or immune surveillance? / Bright, N.A.; Ockleford, C.D.; Anwar, M.

In: Journal of Anatomy, Vol. 184, No. 2, 1994, p. 297-308.

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@article{e031fed9dcc0488cb34f7346033a2293,
title = "Ontogeny and distribution of Fcγ receptors in the human placenta: Transport or immune surveillance?",
abstract = "The human fetus acquires maternal IgG via the chorioallantoic placenta. Utilising antibodies against 3 characterised subtypes of IgG Fc receptor (FcγR) expressed by human leucocytes, we show by confocal immunofluorescence microscopy that these molecules are also expressed by cells of the placenta. FcγRI (CD64) is expressed by undifferentiated mesenchymal or fibroblast cells of 1st trimester and term chorionic villi. Punctate immunoreactivity for FcγRII (CDw32) is found on capillary endothelial cells of term and 1st trimester villi. FcγRIII (CD16) expression is observed in the trophoblast surrounding chorionic villi that forms the functional 'barrier' between mother and fetus. In 1st trimester villi this receptor is associated with a population of marginated vesicular inclusions of the syncytiotrophoblast. In term villi the receptor is concentrated in the apex of the syncytiotrophoblast, suggesting a possible role in the maternofetal transmission of passive immunity. All 3 subtypes of receptor are expressed by Hofbauer cells. We have been unable to demonstrate these receptors in cytotrophoblast cells. Results obtained using immunofluorescence and immunoelectron microscopic detection of endogenous IgG are consistent with the hypothesis that IgG is internalised into clathrin-coated pits and vesicles. Endogenous IgG was not demonstrable in cytotrophoblast cells. The significance of our inability to demonstrate IgG or specific receptor molecules for IgG in cytotrophoblast cells, and possible roles of Fcγ receptor-bearing cells of the placenta are discussed.",
keywords = "Hofbauer cells, Immunoglobulin G, Syncytiotrophoblast, Trophoblast, Fc receptor, immunoglobulin g, article, chorioallantois, chorion villus, fibroblast, first trimester pregnancy, human, human tissue, immunoelectron microscopy, immunofluorescence microscopy, leukocyte, mesenchyme, ontogeny, passive immunization, placenta, priority journal, protein synthesis, syncytiotrophoblast, trophoblast, vertical transmission, Chorionic Villi, Female, Fibroblasts, Fluorescent Antibody Technique, Human, Mesoderm, Microscopy, Immunoelectron, Placenta, Pregnancy, Receptors, IgG, Support, Non-U.S. Gov't, Trophoblasts",
author = "N.A. Bright and C.D. Ockleford and M. Anwar",
year = "1994",
language = "English",
volume = "184",
pages = "297--308",
journal = "Journal of Anatomy",
issn = "0021-8782",
publisher = "Wiley-Blackwell",
number = "2",

}

RIS

TY - JOUR

T1 - Ontogeny and distribution of Fcγ receptors in the human placenta: Transport or immune surveillance?

AU - Bright, N.A.

AU - Ockleford, C.D.

AU - Anwar, M.

PY - 1994

Y1 - 1994

N2 - The human fetus acquires maternal IgG via the chorioallantoic placenta. Utilising antibodies against 3 characterised subtypes of IgG Fc receptor (FcγR) expressed by human leucocytes, we show by confocal immunofluorescence microscopy that these molecules are also expressed by cells of the placenta. FcγRI (CD64) is expressed by undifferentiated mesenchymal or fibroblast cells of 1st trimester and term chorionic villi. Punctate immunoreactivity for FcγRII (CDw32) is found on capillary endothelial cells of term and 1st trimester villi. FcγRIII (CD16) expression is observed in the trophoblast surrounding chorionic villi that forms the functional 'barrier' between mother and fetus. In 1st trimester villi this receptor is associated with a population of marginated vesicular inclusions of the syncytiotrophoblast. In term villi the receptor is concentrated in the apex of the syncytiotrophoblast, suggesting a possible role in the maternofetal transmission of passive immunity. All 3 subtypes of receptor are expressed by Hofbauer cells. We have been unable to demonstrate these receptors in cytotrophoblast cells. Results obtained using immunofluorescence and immunoelectron microscopic detection of endogenous IgG are consistent with the hypothesis that IgG is internalised into clathrin-coated pits and vesicles. Endogenous IgG was not demonstrable in cytotrophoblast cells. The significance of our inability to demonstrate IgG or specific receptor molecules for IgG in cytotrophoblast cells, and possible roles of Fcγ receptor-bearing cells of the placenta are discussed.

AB - The human fetus acquires maternal IgG via the chorioallantoic placenta. Utilising antibodies against 3 characterised subtypes of IgG Fc receptor (FcγR) expressed by human leucocytes, we show by confocal immunofluorescence microscopy that these molecules are also expressed by cells of the placenta. FcγRI (CD64) is expressed by undifferentiated mesenchymal or fibroblast cells of 1st trimester and term chorionic villi. Punctate immunoreactivity for FcγRII (CDw32) is found on capillary endothelial cells of term and 1st trimester villi. FcγRIII (CD16) expression is observed in the trophoblast surrounding chorionic villi that forms the functional 'barrier' between mother and fetus. In 1st trimester villi this receptor is associated with a population of marginated vesicular inclusions of the syncytiotrophoblast. In term villi the receptor is concentrated in the apex of the syncytiotrophoblast, suggesting a possible role in the maternofetal transmission of passive immunity. All 3 subtypes of receptor are expressed by Hofbauer cells. We have been unable to demonstrate these receptors in cytotrophoblast cells. Results obtained using immunofluorescence and immunoelectron microscopic detection of endogenous IgG are consistent with the hypothesis that IgG is internalised into clathrin-coated pits and vesicles. Endogenous IgG was not demonstrable in cytotrophoblast cells. The significance of our inability to demonstrate IgG or specific receptor molecules for IgG in cytotrophoblast cells, and possible roles of Fcγ receptor-bearing cells of the placenta are discussed.

KW - Hofbauer cells

KW - Immunoglobulin G

KW - Syncytiotrophoblast

KW - Trophoblast

KW - Fc receptor

KW - immunoglobulin g

KW - article

KW - chorioallantois

KW - chorion villus

KW - fibroblast

KW - first trimester pregnancy

KW - human

KW - human tissue

KW - immunoelectron microscopy

KW - immunofluorescence microscopy

KW - leukocyte

KW - mesenchyme

KW - ontogeny

KW - passive immunization

KW - placenta

KW - priority journal

KW - protein synthesis

KW - syncytiotrophoblast

KW - trophoblast

KW - vertical transmission

KW - Chorionic Villi

KW - Female

KW - Fibroblasts

KW - Fluorescent Antibody Technique

KW - Human

KW - Mesoderm

KW - Microscopy, Immunoelectron

KW - Placenta

KW - Pregnancy

KW - Receptors, IgG

KW - Support, Non-U.S. Gov't

KW - Trophoblasts

M3 - Journal article

VL - 184

SP - 297

EP - 308

JO - Journal of Anatomy

JF - Journal of Anatomy

SN - 0021-8782

IS - 2

ER -