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Optimisation of protein microarray techniques for analysis of the plasma proteome: Minimisation of non-specific binding interactions

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Optimisation of protein microarray techniques for analysis of the plasma proteome: Minimisation of non-specific binding interactions. / Richens, Joanna L; Lunt, Elizabeth A M; O'Shea, Paul.
In: International Immunopharmacology, Vol. 24, No. 2, 02.2015, p. 166-168.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Richens, JL, Lunt, EAM & O'Shea, P 2015, 'Optimisation of protein microarray techniques for analysis of the plasma proteome: Minimisation of non-specific binding interactions', International Immunopharmacology, vol. 24, no. 2, pp. 166-168. https://doi.org/10.1016/j.intimp.2014.12.006

APA

Richens, J. L., Lunt, E. A. M., & O'Shea, P. (2015). Optimisation of protein microarray techniques for analysis of the plasma proteome: Minimisation of non-specific binding interactions. International Immunopharmacology, 24(2), 166-168. https://doi.org/10.1016/j.intimp.2014.12.006

Vancouver

Richens JL, Lunt EAM, O'Shea P. Optimisation of protein microarray techniques for analysis of the plasma proteome: Minimisation of non-specific binding interactions. International Immunopharmacology. 2015 Feb;24(2):166-168. Epub 2014 Dec 11. doi: 10.1016/j.intimp.2014.12.006

Author

Richens, Joanna L ; Lunt, Elizabeth A M ; O'Shea, Paul. / Optimisation of protein microarray techniques for analysis of the plasma proteome : Minimisation of non-specific binding interactions. In: International Immunopharmacology. 2015 ; Vol. 24, No. 2. pp. 166-168.

Bibtex

@article{706d11fd94834ea7a1b53075487283ee,
title = "Optimisation of protein microarray techniques for analysis of the plasma proteome: Minimisation of non-specific binding interactions",
abstract = "Components of the plasma proteome, particularly serum albumin, have been shown to compromise the accuracy of protein microarray technologies through non-specific binding interactions. Optimisation of array conditions is imperative to help address these problems. Here we demonstrate how modifications to array printing conditions and processing methodology can influence the reliability of data output. In particular, we demonstrate that whilst some glycerol is necessary to maintain specific binding signals, it also increases non-specific binding of albumin. Concentrations of 20% glycerol in the printing buffers are therefore recommended. The findings presented here provide opportunities for increased accuracy in plasma protein detection for possible future diagnostic applications.",
keywords = "Albumin, Glycerol, Microarray, Plasma proteome",
author = "Richens, {Joanna L} and Lunt, {Elizabeth A M} and Paul O'Shea",
year = "2015",
month = feb,
doi = "10.1016/j.intimp.2014.12.006",
language = "English",
volume = "24",
pages = "166--168",
journal = "International Immunopharmacology",
issn = "1567-5769",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Optimisation of protein microarray techniques for analysis of the plasma proteome

T2 - Minimisation of non-specific binding interactions

AU - Richens, Joanna L

AU - Lunt, Elizabeth A M

AU - O'Shea, Paul

PY - 2015/2

Y1 - 2015/2

N2 - Components of the plasma proteome, particularly serum albumin, have been shown to compromise the accuracy of protein microarray technologies through non-specific binding interactions. Optimisation of array conditions is imperative to help address these problems. Here we demonstrate how modifications to array printing conditions and processing methodology can influence the reliability of data output. In particular, we demonstrate that whilst some glycerol is necessary to maintain specific binding signals, it also increases non-specific binding of albumin. Concentrations of 20% glycerol in the printing buffers are therefore recommended. The findings presented here provide opportunities for increased accuracy in plasma protein detection for possible future diagnostic applications.

AB - Components of the plasma proteome, particularly serum albumin, have been shown to compromise the accuracy of protein microarray technologies through non-specific binding interactions. Optimisation of array conditions is imperative to help address these problems. Here we demonstrate how modifications to array printing conditions and processing methodology can influence the reliability of data output. In particular, we demonstrate that whilst some glycerol is necessary to maintain specific binding signals, it also increases non-specific binding of albumin. Concentrations of 20% glycerol in the printing buffers are therefore recommended. The findings presented here provide opportunities for increased accuracy in plasma protein detection for possible future diagnostic applications.

KW - Albumin

KW - Glycerol

KW - Microarray

KW - Plasma proteome

U2 - 10.1016/j.intimp.2014.12.006

DO - 10.1016/j.intimp.2014.12.006

M3 - Journal article

C2 - 25497232

VL - 24

SP - 166

EP - 168

JO - International Immunopharmacology

JF - International Immunopharmacology

SN - 1567-5769

IS - 2

ER -