Home > Research > Publications & Outputs > Optimized methods for detecting Salmonella Typh...

Links

Text available via DOI:

View graph of relations

Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Published

Standard

Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches. / Rigby, J.; Elmerhebi, E.; Diness, Y. et al.
In: Journal of Applied Microbiology, Vol. 132, No. 2, 28.02.2022, p. 1503-1517.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Rigby, J, Elmerhebi, E, Diness, Y, Mkwanda, C, Tonthola, K, Galloway, H, Miles, R, Henrion, MYR, Edwards, T, Gauld, J, Msefula, C, Johnston, R, Nair, S, Feasey, N & Elviss, NC 2022, 'Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches', Journal of Applied Microbiology, vol. 132, no. 2, pp. 1503-1517. https://doi.org/10.1111/jam.15237

APA

Rigby, J., Elmerhebi, E., Diness, Y., Mkwanda, C., Tonthola, K., Galloway, H., Miles, R., Henrion, M. Y. R., Edwards, T., Gauld, J., Msefula, C., Johnston, R., Nair, S., Feasey, N., & Elviss, N. C. (2022). Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches. Journal of Applied Microbiology, 132(2), 1503-1517. https://doi.org/10.1111/jam.15237

Vancouver

Rigby J, Elmerhebi E, Diness Y, Mkwanda C, Tonthola K, Galloway H et al. Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches. Journal of Applied Microbiology. 2022 Feb 28;132(2):1503-1517. Epub 2021 Aug 24. doi: 10.1111/jam.15237

Author

Rigby, J. ; Elmerhebi, E. ; Diness, Y. et al. / Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches. In: Journal of Applied Microbiology. 2022 ; Vol. 132, No. 2. pp. 1503-1517.

Bibtex

@article{cb24dc75bf56451a94f50180ac6e5930,
title = "Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches",
abstract = "Aims: This study evaluated detection methods for Salmonella Typhi (S. Typhi) in the environment, to establish a novel pathway from field sampling to isolation of viable organisms and molecular confirmation from complex environmental samples, thus enabling environmental surveillance of typhoid. Methods and Results: Multiple media were assessed using clinical isolates from the Public Health England's (PHE) Culture collection. The culture pathway selected consisted of a primary 2% bile broth and secondary Selenite F broth, followed by modified Chromogenic Agar for Salmonella Esterase (mCASE). A qPCR assay was adapted from a validated S. Typhi PCR panel for confirmation of isolates, with comparison to biochemical and serological tests showing good specificity. Sampling locations in Blantyre, Malawi were used to compare sampling methods. Viable S. Typhi were isolated from a mixture of trap and grab river water samples on six occasions. Conclusions: Culture of viable S. Typhi from environmental samples was possible using effective capture and culture techniques. Significance and impact of study: Whilst several studies have attempted to detect S. Typhi from the environment, this is the first successful attempt to isolate the organism from river water since the 1980s. Supplementing clinical data with environmental screening offers the potential for enhanced surveillance, which might inform interventions and assess vaccination programmes.  ",
keywords = "bile broth, biofilm, biofilms, Identification, Malawi, mCASE, Moore swabs, PCR (polymerase chain reaction), river water, salmonella, selenite broth, typhoid, water",
author = "J. Rigby and E. Elmerhebi and Y. Diness and C. Mkwanda and K. Tonthola and H. Galloway and R. Miles and M.Y.R. Henrion and T. Edwards and J. Gauld and C. Msefula and R. Johnston and S. Nair and N. Feasey and N.C. Elviss",
year = "2022",
month = feb,
day = "28",
doi = "10.1111/jam.15237",
language = "English",
volume = "132",
pages = "1503--1517",
journal = "Journal of Applied Microbiology",
issn = "1364-5072",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Optimized methods for detecting Salmonella Typhi in the environment using validated field sampling, culture and confirmatory molecular approaches

AU - Rigby, J.

AU - Elmerhebi, E.

AU - Diness, Y.

AU - Mkwanda, C.

AU - Tonthola, K.

AU - Galloway, H.

AU - Miles, R.

AU - Henrion, M.Y.R.

AU - Edwards, T.

AU - Gauld, J.

AU - Msefula, C.

AU - Johnston, R.

AU - Nair, S.

AU - Feasey, N.

AU - Elviss, N.C.

PY - 2022/2/28

Y1 - 2022/2/28

N2 - Aims: This study evaluated detection methods for Salmonella Typhi (S. Typhi) in the environment, to establish a novel pathway from field sampling to isolation of viable organisms and molecular confirmation from complex environmental samples, thus enabling environmental surveillance of typhoid. Methods and Results: Multiple media were assessed using clinical isolates from the Public Health England's (PHE) Culture collection. The culture pathway selected consisted of a primary 2% bile broth and secondary Selenite F broth, followed by modified Chromogenic Agar for Salmonella Esterase (mCASE). A qPCR assay was adapted from a validated S. Typhi PCR panel for confirmation of isolates, with comparison to biochemical and serological tests showing good specificity. Sampling locations in Blantyre, Malawi were used to compare sampling methods. Viable S. Typhi were isolated from a mixture of trap and grab river water samples on six occasions. Conclusions: Culture of viable S. Typhi from environmental samples was possible using effective capture and culture techniques. Significance and impact of study: Whilst several studies have attempted to detect S. Typhi from the environment, this is the first successful attempt to isolate the organism from river water since the 1980s. Supplementing clinical data with environmental screening offers the potential for enhanced surveillance, which might inform interventions and assess vaccination programmes.  

AB - Aims: This study evaluated detection methods for Salmonella Typhi (S. Typhi) in the environment, to establish a novel pathway from field sampling to isolation of viable organisms and molecular confirmation from complex environmental samples, thus enabling environmental surveillance of typhoid. Methods and Results: Multiple media were assessed using clinical isolates from the Public Health England's (PHE) Culture collection. The culture pathway selected consisted of a primary 2% bile broth and secondary Selenite F broth, followed by modified Chromogenic Agar for Salmonella Esterase (mCASE). A qPCR assay was adapted from a validated S. Typhi PCR panel for confirmation of isolates, with comparison to biochemical and serological tests showing good specificity. Sampling locations in Blantyre, Malawi were used to compare sampling methods. Viable S. Typhi were isolated from a mixture of trap and grab river water samples on six occasions. Conclusions: Culture of viable S. Typhi from environmental samples was possible using effective capture and culture techniques. Significance and impact of study: Whilst several studies have attempted to detect S. Typhi from the environment, this is the first successful attempt to isolate the organism from river water since the 1980s. Supplementing clinical data with environmental screening offers the potential for enhanced surveillance, which might inform interventions and assess vaccination programmes.  

KW - bile broth

KW - biofilm

KW - biofilms

KW - Identification

KW - Malawi

KW - mCASE

KW - Moore swabs

KW - PCR (polymerase chain reaction)

KW - river water

KW - salmonella

KW - selenite broth

KW - typhoid

KW - water

U2 - 10.1111/jam.15237

DO - 10.1111/jam.15237

M3 - Journal article

VL - 132

SP - 1503

EP - 1517

JO - Journal of Applied Microbiology

JF - Journal of Applied Microbiology

SN - 1364-5072

IS - 2

ER -