Rights statement: This is the author’s version of a work that was accepted for publication in Carbohydrate Polymers. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate Polymers, 205, 2019 DOI: 10.1016/j.carbpol.2018.10.061
Accepted author manuscript, 1.69 MB, PDF document
Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Pectin-bioactive glass self-gelling, injectable composites with high antibacterial activity
AU - Douglas, Timothy Edward Lim
AU - Dziadek, Michal
AU - Schietse, Josefien
AU - Boone, Matthieu
AU - Declercq, Heidi
AU - Coenye, Tom
AU - Vanhoorne, Valerie
AU - Vervaet, Chris
AU - Balcaen, Lieve
AU - Buchweitz, Maria
AU - Vanhaecke, Frank
AU - Van Assche, Frederic
AU - Cholewa-Kowalska, Katarzyna
AU - Skirtach, Andre G.
N1 - This is the author’s version of a work that was accepted for publication in Carbohydrate Polymers. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbohydrate Polymers, 205, 2019 DOI: 10.1016/j.carbpol.2018.10.061
PY - 2019/2/1
Y1 - 2019/2/1
N2 - The present work focuses on the development of novel injectable, self-gelling composite hydrogels based on two types of low esterified amidated pectins from citrus peels and apple pomace. Sol-gelderived, calcium-rich bioactive glass (BG) fillers in a particle form are applied as delivery vehicles for the release of Ca2+ ions to induce internal gelation of pectins. Composites were prepared by a relatively simple mixing technique, using 20% w/v BG particles of two different sizes (2.5 and <45 µm). Smaller particles accelerated pectin gelation slightly faster than bigger ones, which appears to result from the higher rate of Ca2+ ion release. µCT showed inhomogeneous distribution of the BG particles within the hydrogels. All composite hydrogels exhibited strong antibacterial activity against methicilin-resistant Staphylococcus aureus. The mineralization process of pectin-BG composite hydrogels occurred upon incubation in simulated body fluid for 28 days. In vitro studies demonstrated cytocompatibility of composite hydrogels with MC3T3-E1 osteoblastic cells.
AB - The present work focuses on the development of novel injectable, self-gelling composite hydrogels based on two types of low esterified amidated pectins from citrus peels and apple pomace. Sol-gelderived, calcium-rich bioactive glass (BG) fillers in a particle form are applied as delivery vehicles for the release of Ca2+ ions to induce internal gelation of pectins. Composites were prepared by a relatively simple mixing technique, using 20% w/v BG particles of two different sizes (2.5 and <45 µm). Smaller particles accelerated pectin gelation slightly faster than bigger ones, which appears to result from the higher rate of Ca2+ ion release. µCT showed inhomogeneous distribution of the BG particles within the hydrogels. All composite hydrogels exhibited strong antibacterial activity against methicilin-resistant Staphylococcus aureus. The mineralization process of pectin-BG composite hydrogels occurred upon incubation in simulated body fluid for 28 days. In vitro studies demonstrated cytocompatibility of composite hydrogels with MC3T3-E1 osteoblastic cells.
KW - Antibacterial
KW - Bioactive glass
KW - Bone tissue engineering
KW - Hydrogel
KW - Pectin
KW - Bacteria
KW - Gelation
KW - Hydrogels
KW - Sol-gels
KW - Tissue engineering
KW - Anti-bacterial activity
KW - Inhomogeneous distribution
KW - Methicilin resistant staphylococcus aureus
KW - Mineralization process
KW - Simulated body fluids
U2 - 10.1016/j.carbpol.2018.10.061
DO - 10.1016/j.carbpol.2018.10.061
M3 - Journal article
VL - 205
SP - 427
EP - 436
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
SN - 0144-8617
ER -