TY - GEN

T1 - Plasmon-based determination of macromolecular interactions with membrane-encapsulated nanoparticles

AU - O'Shea, Paul

AU - Richens, Joanna L.

AU - Bramble, Jonathan P.

AU - Bain, jennifer

PY - 2018/5/15

Y1 - 2018/5/15

N2 - Nanoparticles exhibit various optical properties arising from scattering and absorption due to polariton excitation. The resulting frequency and amplitude is dependent on several factors such as particle size, shape, and dielectric environment. By modifying the environment of the nanoparticle surface, in particular by encapsulating an individual nanoparticle within a membrane bilayer comprising defined phospholipids, these properties may be utilised to interrogate molecular interactions adjacent to the particle surface to useful levels of sensitivity. We describe the underlying rationale of these properties and characterise the preparation and behaviour of the nanoparticles. We indicate the potential this approach may have for sensing and screening in analytical biomolecular technology by demonstrating that it can be utilised to reveal the kinetics of the molecular interactions of membrane associated events. We also indicate that the technique may yield higherorder structural information of the macromolecule-membrane interactions in a highly sensitive manner and discuss the physical origins of these potentially more exotic phenomena.

AB - Nanoparticles exhibit various optical properties arising from scattering and absorption due to polariton excitation. The resulting frequency and amplitude is dependent on several factors such as particle size, shape, and dielectric environment. By modifying the environment of the nanoparticle surface, in particular by encapsulating an individual nanoparticle within a membrane bilayer comprising defined phospholipids, these properties may be utilised to interrogate molecular interactions adjacent to the particle surface to useful levels of sensitivity. We describe the underlying rationale of these properties and characterise the preparation and behaviour of the nanoparticles. We indicate the potential this approach may have for sensing and screening in analytical biomolecular technology by demonstrating that it can be utilised to reveal the kinetics of the molecular interactions of membrane associated events. We also indicate that the technique may yield higherorder structural information of the macromolecule-membrane interactions in a highly sensitive manner and discuss the physical origins of these potentially more exotic phenomena.

U2 - https://doi.org/10.1117/12.2306974

DO - https://doi.org/10.1117/12.2306974

M3 - Conference contribution/Paper

BT - Proceedings of SPIE Volume 10672

PB - SPIE

ER -