Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major
AU - Jamjoom, Manal
AU - Ashford, R W
AU - Bates, Paul
AU - Kemp, Stephen J
AU - Noyes, Harry A
PY - 2002/6
Y1 - 2002/6
N2 - Thirteen sets of polymerase chain reaction (PCR) primers were designed to amplify microsatellite loci identified in the genome sequence of Leishmania major. Polymorphisms were detected in L. major at all loci. In Leishmania donovani only two of these loci were informative for classification purposes with this data set. The PCR products of all loci from one L. donovani strain were sequenced and it was found that the number of repeats in the microsatellite loci were either substantially reduced with respect to L. major or absent altogether. Consequently it is unlikely to be possible to use the genome sequence of L. major to identify polymorphic microsatellite loci in other Leishmania species.
AB - Thirteen sets of polymerase chain reaction (PCR) primers were designed to amplify microsatellite loci identified in the genome sequence of Leishmania major. Polymorphisms were detected in L. major at all loci. In Leishmania donovani only two of these loci were informative for classification purposes with this data set. The PCR products of all loci from one L. donovani strain were sequenced and it was found that the number of repeats in the microsatellite loci were either substantially reduced with respect to L. major or absent altogether. Consequently it is unlikely to be possible to use the genome sequence of L. major to identify polymorphic microsatellite loci in other Leishmania species.
KW - Leishmania tropica
KW - Leishmania mexicana
KW - mapping
KW - Leishmania infantum
U2 - 10.1046/j.1471-8286.2002.00161.x
DO - 10.1046/j.1471-8286.2002.00161.x
M3 - Journal article
VL - 2
SP - 104
EP - 106
JO - Molecular Ecology Notes
JF - Molecular Ecology Notes
SN - 1471-8286
IS - 2
ER -