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Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration. / Qasim, S.B.; Najeeb, S.; Delaine-Smith, R.M. et al.
In: Dental Materials, Vol. 33, No. 1, 01.01.2017, p. 71-83.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Qasim, SB, Najeeb, S, Delaine-Smith, RM, Rawlinson, A & Ur Rehman, I 2017, 'Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration', Dental Materials, vol. 33, no. 1, pp. 71-83. https://doi.org/10.1016/j.dental.2016.10.003

APA

Qasim, S. B., Najeeb, S., Delaine-Smith, R. M., Rawlinson, A., & Ur Rehman, I. (2017). Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration. Dental Materials, 33(1), 71-83. https://doi.org/10.1016/j.dental.2016.10.003

Vancouver

Qasim SB, Najeeb S, Delaine-Smith RM, Rawlinson A, Ur Rehman I. Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration. Dental Materials. 2017 Jan 1;33(1):71-83. Epub 2016 Nov 11. doi: 10.1016/j.dental.2016.10.003

Author

Qasim, S.B. ; Najeeb, S. ; Delaine-Smith, R.M. et al. / Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration. In: Dental Materials. 2017 ; Vol. 33, No. 1. pp. 71-83.

Bibtex

@article{e60916d19e7f4ecd814e2c29b2e8beb6,
title = "Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration",
abstract = "Objective The regeneration of periodontal tissues lost as a consequence of destructive periodontal disease remains a challenge for clinicians. Guided tissue regeneration (GTR) has emerged as the most widely practiced regenerative procedure. Aim of this study was to electrospin chitosan (CH) membranes with a low or high degree of fiber orientation and examines their suitability for use as a surface layer in GTR membranes, which can ease integration with the periodontal tissue by controlling the direction of cell growth. Methods A solution of CH-doped with polyethylene oxide (PEO) (ratio 95:5) was prepared for electrospinning. Characterization was performed for biophysiochemical and mechanical properties by means of scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, swelling ratio, tensile testing and monitoring degradation using pH analysis, weight profile, ultraviolet–visible (UV–vis) spectroscopy and FTIR analysis. Obtained fibers were also assessed for viability and matrix deposition using human osteosarcoma (MG63) and human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells. Results Random and aligned CH fibers were obtained. FTIR analysis showed neat CH spectral profile before and after electrospinning. Electropsun mats were conducive to cellular attachment and viability increased with time. The fibers supported matrix deposition by hES-MPs. Histological sections showed cellular infiltration as well. Significance The surface layer would act as seal to prevent junctional epithelium from falling into the defect site and hence maintain space for bone regeneration. {\textcopyright} 2016 The Academy of Dental Materials",
keywords = "CH, Characterization, Dental biomaterials, Electrospinning, Functionally graded, GTR, hES-MPs, MG63, PEO, Periodontal, Biomechanics, Cell growth, Chitin, Chitosan, Deposition, Fibers, Fourier transform infrared spectroscopy, Polyethylene oxides, Scanning electron microscopy, Spinning (fibers), Stem cells, Surface defects, Tensile testing, Tissue, Guided tissue regeneration, Human embryonic stem cells, Mesenchymal progenitors, Polyethylene oxide (PEO), Tissue regeneration, chitosan, artificial membrane, bone regeneration, human, male, periodontal guided tissue regeneration, periodontium, tissue regeneration, Bone Regeneration, Guided Tissue Regeneration, Guided Tissue Regeneration, Periodontal, Humans, Male, Membranes, Artificial, Periodontium",
author = "S.B. Qasim and S. Najeeb and R.M. Delaine-Smith and A. Rawlinson and {Ur Rehman}, I.",
year = "2017",
month = jan,
day = "1",
doi = "10.1016/j.dental.2016.10.003",
language = "English",
volume = "33",
pages = "71--83",
journal = "Dental Materials",
issn = "0109-5641",
publisher = "Elsevier Inc.",
number = "1",

}

RIS

TY - JOUR

T1 - Potential of electrospun chitosan fibers as a surface layer in functionally graded GTR membrane for periodontal regeneration

AU - Qasim, S.B.

AU - Najeeb, S.

AU - Delaine-Smith, R.M.

AU - Rawlinson, A.

AU - Ur Rehman, I.

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Objective The regeneration of periodontal tissues lost as a consequence of destructive periodontal disease remains a challenge for clinicians. Guided tissue regeneration (GTR) has emerged as the most widely practiced regenerative procedure. Aim of this study was to electrospin chitosan (CH) membranes with a low or high degree of fiber orientation and examines their suitability for use as a surface layer in GTR membranes, which can ease integration with the periodontal tissue by controlling the direction of cell growth. Methods A solution of CH-doped with polyethylene oxide (PEO) (ratio 95:5) was prepared for electrospinning. Characterization was performed for biophysiochemical and mechanical properties by means of scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, swelling ratio, tensile testing and monitoring degradation using pH analysis, weight profile, ultraviolet–visible (UV–vis) spectroscopy and FTIR analysis. Obtained fibers were also assessed for viability and matrix deposition using human osteosarcoma (MG63) and human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells. Results Random and aligned CH fibers were obtained. FTIR analysis showed neat CH spectral profile before and after electrospinning. Electropsun mats were conducive to cellular attachment and viability increased with time. The fibers supported matrix deposition by hES-MPs. Histological sections showed cellular infiltration as well. Significance The surface layer would act as seal to prevent junctional epithelium from falling into the defect site and hence maintain space for bone regeneration. © 2016 The Academy of Dental Materials

AB - Objective The regeneration of periodontal tissues lost as a consequence of destructive periodontal disease remains a challenge for clinicians. Guided tissue regeneration (GTR) has emerged as the most widely practiced regenerative procedure. Aim of this study was to electrospin chitosan (CH) membranes with a low or high degree of fiber orientation and examines their suitability for use as a surface layer in GTR membranes, which can ease integration with the periodontal tissue by controlling the direction of cell growth. Methods A solution of CH-doped with polyethylene oxide (PEO) (ratio 95:5) was prepared for electrospinning. Characterization was performed for biophysiochemical and mechanical properties by means of scanning electron microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, swelling ratio, tensile testing and monitoring degradation using pH analysis, weight profile, ultraviolet–visible (UV–vis) spectroscopy and FTIR analysis. Obtained fibers were also assessed for viability and matrix deposition using human osteosarcoma (MG63) and human embryonic stem cell-derived mesenchymal progenitor (hES-MP) cells. Results Random and aligned CH fibers were obtained. FTIR analysis showed neat CH spectral profile before and after electrospinning. Electropsun mats were conducive to cellular attachment and viability increased with time. The fibers supported matrix deposition by hES-MPs. Histological sections showed cellular infiltration as well. Significance The surface layer would act as seal to prevent junctional epithelium from falling into the defect site and hence maintain space for bone regeneration. © 2016 The Academy of Dental Materials

KW - CH

KW - Characterization

KW - Dental biomaterials

KW - Electrospinning

KW - Functionally graded

KW - GTR

KW - hES-MPs

KW - MG63

KW - PEO

KW - Periodontal

KW - Biomechanics

KW - Cell growth

KW - Chitin

KW - Chitosan

KW - Deposition

KW - Fibers

KW - Fourier transform infrared spectroscopy

KW - Polyethylene oxides

KW - Scanning electron microscopy

KW - Spinning (fibers)

KW - Stem cells

KW - Surface defects

KW - Tensile testing

KW - Tissue

KW - Guided tissue regeneration

KW - Human embryonic stem cells

KW - Mesenchymal progenitors

KW - Polyethylene oxide (PEO)

KW - Tissue regeneration

KW - chitosan

KW - artificial membrane

KW - bone regeneration

KW - human

KW - male

KW - periodontal guided tissue regeneration

KW - periodontium

KW - tissue regeneration

KW - Bone Regeneration

KW - Guided Tissue Regeneration

KW - Guided Tissue Regeneration, Periodontal

KW - Humans

KW - Male

KW - Membranes, Artificial

KW - Periodontium

U2 - 10.1016/j.dental.2016.10.003

DO - 10.1016/j.dental.2016.10.003

M3 - Journal article

VL - 33

SP - 71

EP - 83

JO - Dental Materials

JF - Dental Materials

SN - 0109-5641

IS - 1

ER -