Premature dissolution of the microsporocyte callose wall causes male-sterility in transgenic tobacco

Lancaster Environment Centre

Keywords

CYTOPLASMIC MALE-STERILE, MALE-FERTILE, COMPARATIVE LIGHT, BRASSICA-NAPUS, PETUNIA, ANTHERS, MICROSPOROGENESIS, PLANTS, ENZYME, EXINE

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Published

Standard

Premature dissolution of the microsporocyte callose wall causes male-sterility in transgenic tobacco. / WORRALL, D ; HIRD, D L ; HODGE, R et al.
In: Plant Cell, Vol. 4, No. 7, 07.1992, p. 759-771.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Bibtex

@article{ebf0dd5862054279a19104c64cf9f3d8,

title = "Premature dissolution of the microsporocyte callose wall causes male-sterility in transgenic tobacco",

abstract = "Male sterility in a petunia cytoplasmic male sterile line has been attributed to the early appearance of active callase, a beta-1,3-glucanase, in the anther locule. This leads to premature dissolution of the callose walls surrounding the microsporogenous cells. We have mimicked this aspect of the petunia line in transgenic tobacco by engineering the secretion of a modified pathogenesis-related vacuolar beta-1,3-glucanase from the tapetum prior to the appearance of callase activity in the locule. Plants expressing the modified glucanase from tapetum-specific promoters exhibited reduced male fertility, ranging from complete to partial male sterility. Callose appearance and distribution are normal in the male sterile transgenic plants up to prophase 1, whereupon callose is prematurely degraded. Meiosis and cell division occur normally. The resultant microspores have an abnormally thin cell wall that lacks sculpturing. The tapetum shows hypertrophy. Male sterility is probably caused by bursting of the aberrant microspores at a time corresponding to microspore release. These results demonstrate that premature callose degradation is sufficient to cause male sterility and suggest that callose is essential for the formation of a normal microspore cell wall.",

keywords = "CYTOPLASMIC MALE-STERILE, MALE-FERTILE, COMPARATIVE LIGHT, BRASSICA-NAPUS, PETUNIA, ANTHERS, MICROSPOROGENESIS, PLANTS, ENZYME, EXINE",

author = "D WORRALL and HIRD, {D L} and R HODGE and W PAUL and J DRAPER and R SCOTT",

year = "1992",

month = jul,

language = "English",

volume = "4",

pages = "759--771",

journal = "Plant Cell",

issn = "1040-4651",

publisher = "American Society of Plant Biologists",

number = "7",

}

RIS

TY - JOUR

T1 - Premature dissolution of the microsporocyte callose wall causes male-sterility in transgenic tobacco

AU - WORRALL, D

AU - HIRD, D L

AU - HODGE, R

AU - PAUL, W

AU - DRAPER, J

AU - SCOTT, R

PY - 1992/7

Y1 - 1992/7

N2 - Male sterility in a petunia cytoplasmic male sterile line has been attributed to the early appearance of active callase, a beta-1,3-glucanase, in the anther locule. This leads to premature dissolution of the callose walls surrounding the microsporogenous cells. We have mimicked this aspect of the petunia line in transgenic tobacco by engineering the secretion of a modified pathogenesis-related vacuolar beta-1,3-glucanase from the tapetum prior to the appearance of callase activity in the locule. Plants expressing the modified glucanase from tapetum-specific promoters exhibited reduced male fertility, ranging from complete to partial male sterility. Callose appearance and distribution are normal in the male sterile transgenic plants up to prophase 1, whereupon callose is prematurely degraded. Meiosis and cell division occur normally. The resultant microspores have an abnormally thin cell wall that lacks sculpturing. The tapetum shows hypertrophy. Male sterility is probably caused by bursting of the aberrant microspores at a time corresponding to microspore release. These results demonstrate that premature callose degradation is sufficient to cause male sterility and suggest that callose is essential for the formation of a normal microspore cell wall.

AB - Male sterility in a petunia cytoplasmic male sterile line has been attributed to the early appearance of active callase, a beta-1,3-glucanase, in the anther locule. This leads to premature dissolution of the callose walls surrounding the microsporogenous cells. We have mimicked this aspect of the petunia line in transgenic tobacco by engineering the secretion of a modified pathogenesis-related vacuolar beta-1,3-glucanase from the tapetum prior to the appearance of callase activity in the locule. Plants expressing the modified glucanase from tapetum-specific promoters exhibited reduced male fertility, ranging from complete to partial male sterility. Callose appearance and distribution are normal in the male sterile transgenic plants up to prophase 1, whereupon callose is prematurely degraded. Meiosis and cell division occur normally. The resultant microspores have an abnormally thin cell wall that lacks sculpturing. The tapetum shows hypertrophy. Male sterility is probably caused by bursting of the aberrant microspores at a time corresponding to microspore release. These results demonstrate that premature callose degradation is sufficient to cause male sterility and suggest that callose is essential for the formation of a normal microspore cell wall.

KW - CYTOPLASMIC MALE-STERILE

KW - MALE-FERTILE

KW - COMPARATIVE LIGHT

KW - BRASSICA-NAPUS

KW - PETUNIA

KW - ANTHERS

KW - MICROSPOROGENESIS

KW - PLANTS

KW - ENZYME

KW - EXINE

M3 - Journal article

VL - 4

SP - 759

EP - 771

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 7

ER -

Research

Links

Keywords