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Prenylation of the floral transcription factor APETALA1 modulates its function

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Prenylation of the floral transcription factor APETALA1 modulates its function. / Yalovsky, Shaul; Rodríguez-Concepción, Manuel; Bracha, Keren et al.
In: Plant Cell, Vol. 12, No. 8, 08.2000, p. 1257-1266.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Yalovsky, S, Rodríguez-Concepción, M, Bracha, K, Toledo-Ortiz, G & Gruissem, W 2000, 'Prenylation of the floral transcription factor APETALA1 modulates its function', Plant Cell, vol. 12, no. 8, pp. 1257-1266. https://doi.org/10.1105/tpc.12.8.1257

APA

Yalovsky, S., Rodríguez-Concepción, M., Bracha, K., Toledo-Ortiz, G., & Gruissem, W. (2000). Prenylation of the floral transcription factor APETALA1 modulates its function. Plant Cell, 12(8), 1257-1266. https://doi.org/10.1105/tpc.12.8.1257

Vancouver

Yalovsky S, Rodríguez-Concepción M, Bracha K, Toledo-Ortiz G, Gruissem W. Prenylation of the floral transcription factor APETALA1 modulates its function. Plant Cell. 2000 Aug;12(8):1257-1266. Epub 2000 Aug 1. doi: 10.1105/tpc.12.8.1257

Author

Yalovsky, Shaul ; Rodríguez-Concepción, Manuel ; Bracha, Keren et al. / Prenylation of the floral transcription factor APETALA1 modulates its function. In: Plant Cell. 2000 ; Vol. 12, No. 8. pp. 1257-1266.

Bibtex

@article{619bc4f3981443599e2f049bcb3cd2bc,
title = "Prenylation of the floral transcription factor APETALA1 modulates its function",
abstract = "The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo. AP1 regulates the transition from inflorescence shoot to floral meristems and the development of sepals and petals. AP1 fused to green fluorescent protein (GFP) retained transcription factor activity and directed the expected terminal flower phenotype when ectopically expressed in transgenic Arabidopsis. However, ap1mS, a farnesyl cysteine-acceptor mutant of AP1, as well as the GFP-ap1mS fusion protein failed to direct the development of compound terminal flowers but instead induced novel phenotypes when ectopically expressed in Arabidopsis. Similarly, compound terminal flowers did not develop in era1-2 transformants that ectopically expressed AP1. Together, the results demonstrate that AP1 is a target of farnesyltransferase and suggest that farnesylation alters the function and perhaps specificity of the transcription factor.",
keywords = "Alkyl and Aryl Transferases, Amino Acid Motifs, Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Farnesyltranstransferase, Genes, Plant, Homeodomain Proteins, MADS Domain Proteins, Molecular Sequence Data, Mutation, Phenotype, Plant Growth Regulators, Plant Proteins, Plant Structures, Plants, Genetically Modified, Protein Prenylation, Recombinant Fusion Proteins, Sequence Alignment, Transcription Factors",
author = "Shaul Yalovsky and Manuel Rodr{\'i}guez-Concepci{\'o}n and Keren Bracha and Gabriela Toledo-Ortiz and Wilhelm Gruissem",
year = "2000",
month = aug,
doi = "10.1105/tpc.12.8.1257",
language = "English",
volume = "12",
pages = "1257--1266",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "8",

}

RIS

TY - JOUR

T1 - Prenylation of the floral transcription factor APETALA1 modulates its function

AU - Yalovsky, Shaul

AU - Rodríguez-Concepción, Manuel

AU - Bracha, Keren

AU - Toledo-Ortiz, Gabriela

AU - Gruissem, Wilhelm

PY - 2000/8

Y1 - 2000/8

N2 - The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo. AP1 regulates the transition from inflorescence shoot to floral meristems and the development of sepals and petals. AP1 fused to green fluorescent protein (GFP) retained transcription factor activity and directed the expected terminal flower phenotype when ectopically expressed in transgenic Arabidopsis. However, ap1mS, a farnesyl cysteine-acceptor mutant of AP1, as well as the GFP-ap1mS fusion protein failed to direct the development of compound terminal flowers but instead induced novel phenotypes when ectopically expressed in Arabidopsis. Similarly, compound terminal flowers did not develop in era1-2 transformants that ectopically expressed AP1. Together, the results demonstrate that AP1 is a target of farnesyltransferase and suggest that farnesylation alters the function and perhaps specificity of the transcription factor.

AB - The Arabidopsis MADS box transcription factor APETALA1 (AP1) was identified as a substrate for farnesyltransferase and shown to be farnesylated efficiently both in vitro and in vivo. AP1 regulates the transition from inflorescence shoot to floral meristems and the development of sepals and petals. AP1 fused to green fluorescent protein (GFP) retained transcription factor activity and directed the expected terminal flower phenotype when ectopically expressed in transgenic Arabidopsis. However, ap1mS, a farnesyl cysteine-acceptor mutant of AP1, as well as the GFP-ap1mS fusion protein failed to direct the development of compound terminal flowers but instead induced novel phenotypes when ectopically expressed in Arabidopsis. Similarly, compound terminal flowers did not develop in era1-2 transformants that ectopically expressed AP1. Together, the results demonstrate that AP1 is a target of farnesyltransferase and suggest that farnesylation alters the function and perhaps specificity of the transcription factor.

KW - Alkyl and Aryl Transferases

KW - Amino Acid Motifs

KW - Amino Acid Sequence

KW - Arabidopsis

KW - Arabidopsis Proteins

KW - Farnesyltranstransferase

KW - Genes, Plant

KW - Homeodomain Proteins

KW - MADS Domain Proteins

KW - Molecular Sequence Data

KW - Mutation

KW - Phenotype

KW - Plant Growth Regulators

KW - Plant Proteins

KW - Plant Structures

KW - Plants, Genetically Modified

KW - Protein Prenylation

KW - Recombinant Fusion Proteins

KW - Sequence Alignment

KW - Transcription Factors

U2 - 10.1105/tpc.12.8.1257

DO - 10.1105/tpc.12.8.1257

M3 - Journal article

C2 - 10948247

VL - 12

SP - 1257

EP - 1266

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 8

ER -